A kind of antimicrobial peptide for preventing and treating small melon worm

A technology of antimicrobial peptides and melon worms, applied in the direction of peptides, peptide sources, anti-infective drugs, etc., can solve the problems of affecting fish respiration, strong toxicity, carcinogenicity, etc., achieve good killing effect, good stability, and peptide sequence short effect

Active Publication Date: 2020-04-28
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Diseased fish will lose their appetite after being infected with small melon worms. The parasitic gills will cause swelling and necrosis of gill epithelial cells, which will affect the fish's respiration. A large number of deaths caused serious economic losses to farmers
For the treatment of small melon worm disease, the effective therapeutic drugs that have been screened, such as malachite green, mercurous nitrate, and mercurous acetate, are all banned due to strong toxicity, carcinogenicity, and serious environmental pollution.
In recent years, pepper, ginger, gallnut, etc. and potassium permanganate, sodium chloride, formalin, copper sulfate, etc. screened on Chinese herbal medicines have certain curative effects, but most of the drugs only have certain effects on the predatory body stage, and have a certain effect on the nourishment stage. Body stage effect is not significant
At present, there is no ideal drug for the treatment of small melon worms

Method used

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  • A kind of antimicrobial peptide for preventing and treating small melon worm
  • A kind of antimicrobial peptide for preventing and treating small melon worm
  • A kind of antimicrobial peptide for preventing and treating small melon worm

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Development and preparation of embodiment 1 antimicrobial peptide YcHβ

[0016] The skin of fish has been soaked in the water environment containing various bacteria and parasites for a long time. For the scaleless yellow catfish, this layer of mucus covering the body surface is particularly important for resisting infection by exogenous pathogens. We infect the yellow catfish with small melon worms and collect the surviving yellow catfish mucus before and after immunization, then use a freeze dryer to dry the mucus into powder in a vacuum freezing environment, and find the yellow catfish after immunization after dissolving in water The mucus has a killing effect on the trophozoites of the small melon worm. The proteome of the yellow catfish before and after immunization was measured by LC-MS / MS technology, and the proteins that were significantly up-regulated were found. The complete CDs sequence of the corresponding protein was found through the transcriptome and gen...

Embodiment 2

[0018] The control effect of embodiment 2 antibacterial peptide YcHβ to small melon worm

[0019] 1. Materials and methods

[0020] 1.1 Test fish

[0021] The yellow catfish severely infected with the small melon worm was mixed with the normal yellow catfish (28.5±6.7g) in a 60L water tank and inoculated with the small melon worm. 20 fish were randomly selected, and the white cysts on the body surface were clearly visible. The infection rate of the test fish was 100%.

[0022] 1.2 Preparation of trophozoites, cysts and predators

[0023] When the yellow catfish is severely infected by small melon worms (many white spots can be seen with the naked eye on the surface of the fish body), gently use a cell scraper to scrape off the white bag of the fish body bag and collect it in a small beaker, rinse it repeatedly with distilled water for several times until Trophozoites are formed after most of the mucus has been removed. Transfer the small melon worms to another petri dish f...

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PUM

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Abstract

The invention belongs to the technical field of aquaculture and discloses antibacterial peptide YcHbeta. An amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1. The antibacterialpeptide can be used for killing lchthyophthirius parasitizing in freshwater fishes in vitro. Experiments prove that the antibacterial peptide ycHbeta is a natural immune substance from pelteobagrus skin mucus, is nontoxic and harmless to aquatic animals, has high membrane penetrability, is effective on different life history stages of lchthyophthirius and can easily kill trophozoite and predators;thick cysts at a cystic stage can be damaged by using high-concentration antibacterial peptide YcBbeta for treatment, so that wrapped filial-generation polypide can be dissolved.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to an antibacterial peptide for preventing and treating small melon worms in vitro and an application thereof. Background technique [0002] Small melon worm disease, also known as white spot disease, is a common parasitic disease in freshwater fish. There are many small white spots visible to the naked eye on the body surface, gills and fin rays of infected fish. Diseased fish will lose their appetite after being infected with small melon worms. The parasitic gills will cause swelling and necrosis of gill epithelial cells, which will affect the fish's respiration. A large number of deaths have brought serious economic losses to farmers. For the treatment of small melon worm disease, the effective therapeutic drugs that have been screened, such as malachite green, mercurous nitrate, and mercurous acetate, are all banned due to strong toxicity, carcinogenicity, and s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/46A61K38/17A61P33/00
CPCA61K38/00A61P33/00C07K14/461
Inventor 梅洁熊阳皇培培
Owner HUAZHONG AGRI UNIV
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