62 results about "Pelteobagrus eupogon" patented technology

The invention relates to a cultivation method for the summer fingerling of the pelteobagrus. The method comprises the following steps of: (1) selecting a cultivation pond; (2) selecting a water source; (3) preparing the cultivation pond; (4) reasonably stocking; (5) managing the feeding; (6) managing the quality of the water; and (7) preventing and curing the disease and the like. When the method is used for cultivating the summer fingerling of the pelteobagrus, the total artificial forage domestication technologies with different types and different modes are adopted at different times by means of combining with the mode such as eel material slurry sprinkling, dried power sprinkling, bean blocking by powder cluster and the like according to the biologic learnability in different periods to provide a most effective practical measure and method for cultivating the summer fingerling of the pelteobagrus so as to perfect the cultivation technology for the summer fingerling of the pelteobagrus, firstly and successfully break through the key point of the total artificial forage cultivation of the summer fingerling of the pelteobagrus in the domestics, have a theoretical significance and a practice guidance significance for cultivating the artificial forage of the pelteobagrus and improving the forage use ratio, and lay a technical foundation for cultivating the fingerling on a large scale.

The present invention discloses a SNP molecular marker associated with growth traits of Pelteobagrus vachelli, which is located at 41cM position of linkage group 14 of Pelteobagrus vachelli. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO: 1, and a SNP site exists at the 26th position of the gene sequence: the base is G or C. The SNP molecular marker is located at 41cM position of linkage group 14 of Pelteobagrus vachelli. The invention also discloses a specific primer, a kit and a detection method for detecting the molecular marker. It is found that, The genotype ofthe SNP site at 26 bp of the gene at 41 cM in linkage group 14 of Pelteobagrus vachelli is GG, GC or CC is closely related to growth traits of Pelteobagrus vachelli, such as body weight, body width and total length. Therefore, the SNP molecular marker of the invention can be used for detecting or assisting in detecting growth traits of Pelteobagrus vachelli, and can be used for assisting selectionand breeding of Pelteobagrus vachelli. The invention has important guiding significance for breeding new strain of excellent growth characteristics of Pelteobagrus vachelli.

The invention discloses a compound health-caring feed for pelteobagrus fulvidraco and a preparation method thereof. The compound health-caring feed for the pelteobagrus fulvidraco comprises the following ingredients in the following percentage: 10-30% of red snapper meal, 20-35% of soybean meal, 1-5% of fermented soybean meal, 3-10% of rapeseed meal, 3-10% of cottonseed meal, 20-25% of high-gluten flour, 1.0-3.0% of soybean lecithin, 1.0-3.0% of soybean oil; 1.0-3.0% of fish oil, 0.1-0.5% of choline chloride; 1-3% of fish soluble paste, 1.0-2.0% of calcium dihydrogen phosphate, 0.1-0.3% of a mildew proofing agent, 1-4% of beer yeast, 0.5-2% of yeast hydrolysate, 0.1-0.5% of betaine, 0.05-0.2% of chitin; 0.2-0.5% of a vitamin premix for aquaculture, and 1.0-2.0% of a trace element premix for aquaculture. The compound health-caring feed for the pelteobagrus fulvidraco disclosed by the invention is wide and stable in raw material sources, so that preparation cost of the feed prepared by the preparation method is relatively low; moreover, the feed is capable of meeting all nutritional requirements of pelteobagrus fulvidraco cultivation. In addition, the feed is high in feed conversion rate so that rapid growth of the pelteobagrus fulvidraco is ensured; and thus, breeding benefits are high. The feed prepared by the preparation method is also capable of obviously enhancing immune functions and anti-stress abilities of the pelteobagrus fulvidraco fed with the feed; and thus, the adult pelteobagrus fulvidraco is good in vitality, natural in body color and high in survival rate.

The invention relates to the field of aquatic feed processing, and discloses pelteobagrus fulvidraco culturing feed and a preparation method thereof. The feed provided by the invention is prepared from Peru fish meal, bean pulp, flour, soya-bean oil, monocalcium phosphate, zeolite powder, saltwater fish compound premix, feed grade calcium propionate, vitamin C and an antioxidant. The pelteobagrus fulvidraco culturing feed provided by the invention adopts multiple proper components, fish oil commonly used in a traditional feed formula is removed, meanwhile, a cold spraying technology of cooling the feed first and then spraying grease is adopted for preparation, the prepared feed can reduce the feed coefficients and increase the specific growth rate and relative weight gain rate of pelteobagrus fulvidraco.

The invention provides a breeding method of excellent strains of hybrid pelteobagrus fulvidraco resistant to Edwardsiella ictaluri.The method comprises the following steps: collecting the pelteobagrusfulvidraco and pelteobagrus vachelli, cultivating till mature, and then reproducing to obtain the fry of purebred pelteobagrus fulvidraco and the pelteobagrus vachelli with different populations; soaking all the fingerlings of the pelteobagrus fulvidraco and the pelteobagrus vachelli with a soaking method according to determined medial lethal concentration of the Edwardsiella ictaluri; performingbreeding on the population resistant to the Edwardsiella ictaluri twice, screening the excellent disease-resistant strains by the survival rate of mixed-cultured fry, respectively cultivating the strains of the pelteobagrus fulvidraco resistant to the Edwardsiella ictaluri, and the pelteobagrus vachelli to sexual maturity, and then producing the hybrid pelteobagrus fulvidraco according to a universal artificial reproduction method of fish. According to the breeding method of the excellent strains of the hybrid pelteobagrus fulvidraco resistant to the Edwardsiella ictaluri, hole-in-head-disease-resisitant parents of the pelteobagrus fulvidraco and the pelteobagrus vachelli can be cultivated, the incidence and mortality of hole-in-head disease in the seedling and culture process are improved, and the survival rate of the culture is improved.

The invention discloses a pelteobagrus fulvidraco beta defensin gene, and beta defensin antibacterial peptide and application thereof. An in vitro recombinant expression technology is used for successfully expressing a pelteobagrus fulvidraco beta defensin recombinant protein PET-32a-Pf_BD, and antibacterial activity identification results show that the pelteobagrus fulvidraco beta defensin gene has a certain bacteriostasis for staphylococcus aureus in gram-positive bacterium, escherichia coli in gram-negative bacterium, aeromonas hydrophila, edwardsiella ictaluri and flavobacterium columnare. Therefore, a foundation is laid for application of the defensin recombinant protein, used as a broad-spectrum antibacterial drug, in aquaculture.

The invention relates to the technical field of biology and discloses a whole genome cloning method of pelteobagrus fulvidraco bacillus-shaped virus. The method comprises the following steps of: designing 14 specific primers for reverse transcription and 14 pairs of specific primers for PCR, wherein the primer sequences are shown as SEQ ID No.1-SEQ ID No.14; extracting pelteobagrus fulvidraco bacillus-shaped virus RNA; taking the extracted viral RNA as a template, respectively adding the extracted viral RNA into a reverse transcription reaction system and a PCR reaction system by using the primers, cloning the whole genome of the pelteobagrus fulvidraco bacillus-shaped virus by sections under reaction conditions, wherein 13 fragments of the amplified product are 2000bp in size, one fragment is 1000bp in size, a total of 14 fragments contain the entire genome sequence of the pelteobagrus fulvidraco bacillus-shaped virus. The invention provides a cloning method of the whole genome of thepelteobagrus fulvidraco bacillus-shaped virus. The method not only can be used for rapid cloning of the whole genome of the pelteobagrus fulvidraco bacillus-shaped virus, but also can be used for researching molecular biology pathogenic mechanism and molecular epidemiology investigation of the pelteobagrus fulvidraco bacillus-shaped virus, and is convenient to use and has good effect.

The invention relates to a low-fish-meal compound feed for preventing and treating enteritis of pelteobagrus fulvidraco and a preparation method of the low-fish-meal compound feed. The low-fish-meal compound feed comprises the following components in parts by weight: 21-24 parts of fish meal, 6-10 parts of corn protein powder, 12-14 parts of flour, 5-7 parts of chicken meal, 1-2 parts of blood cell protein powder, 4-6 parts of pork powder, 5-7 parts of cottonseed protein powder, 22-27 parts of soybean meal, 6-10 parts of rapeseed meal, 3.6-3.9 parts of soybean oil, 2-6 parts of a premix and 0.1-2 parts of an intestinal function compound. Wherein the intestinal function compound is prepared from the following components in parts by weight: 0.1 to 0.3 part of sodium butyrate, 0.05 to 0.2 part of citric acid, 0.05 to 0.2 part of glutamine and 0.05 to 0.2 part of protease. The low-fish-meal compound feed can ensure the growth function under the condition of low fish meal, the feed cost isreduced, and the culture benefit is improved.

The invention relates to a method for increasing pelteobagrus fulvidraco fry breeding survival rate and a preparation method thereof. The preparation method comprises the following steps: (1) designing a fry breeding pond, (2) designing a bait breeding pond, (3) seeding, absorbing excessive nutrients and fixing the bottom of the fry breeding pond and the bait breeding pond, (4) fertilizing and water culture, (5) throwing fries, (6) controlling diseases and insect pests, (7) supplementing biological baits, (8) feeding feeds and (9) regularly regulating water. The net frame is erected on the periphery of the fry breeding pond, the aeration stones are arranged on the net frame, attachments are provided for fries which just go down to the pond and cannot swim horizontally, and the fries are prevented from sinking into the water bottom and being killed by oxygen deficit.

The invention relates to a feeding device, in particular to an automatic feeding device for pelteobagrus fulvidraco culture. According to the automatic feeding device for pelteobagrus fulvidraco culture, when feed is fed, the amount of the feed falling each time is consistent, and the feed cannot be caked. The automatic feeding device for pelteobagrus fulvidraco culture comprises a pulling rope and a storage mechanism; a floating mechanism is connected to one end of the pulling rope, and the storage mechanism is arranged on the floating mechanism. Through the floating mechanism and the storagemechanism, the device can float around under the action of water flow, and meanwhile, the feed leaks, so that the condition that a large amount of pelteobagrus fulvidraco is piled together to snatchthe feed is avoided; and the feed can be prevented from being solidified in a storage box through a driving mechanism and a stirring mechanism.

The invention discloses a preparation method of N4-acetyl-sulfamethoxazole residue standard substance in pelteobagrus fulvidraco tissue. The preparation method includes: A, selecting female pelteobagrus fulvidraco which is determined without containing sulfamethoxazole and N4-acetyl-sulfamethoxazole; B, using dimethyl formamide to dissolve sulfamethoxazole, and using water for dilution to obtain a sulfamethoxazole solution; C, orally pouring the sulfamethoxazole solution according to dosage of 60 mg/kg body weight of pelteobagrus fulvidraco, and putting pelteobagrus fulvidraco after going through oral administration in water for observation; D, selecting pelteobagrus fulvidraco without vomiting to prepare N4-acetyl-sulfamethoxazole residue standard substance, collecting back muscle, liver, kidney, gill and ovary of pelteobagrus fulvidraco for homogenization respectively to prepare the N4-acetyl-sulfamethoxazole residue standard substance in the pelteobagrus fulvidraco tissue, freezing and storing the same. The preparation method is easy to implement, simple and convenient to operate and capable of valuing the standard substance prepared and testing uniformity and stability.

The invention discloses a preparation for improving fish intestinal pathology injury. The preparation disclosed by the invention is prepared from the following components in parts by weight: 1 part ofmangosteen extract and 3 parts of stevia rebaudian extract. When the preparation is added into a feed in proportion or mixed with the feed for use, the preparation has remarkable repairing and improving effects on damaged intestinal tracts of cultured animals and has no adverse effect on the production performance of aquatic animals. The preparation is added into feed according to the proportionof 0.1%, so that the intestinal injury of pelteobagrus fulvidraco caused by histamine can be remarkably repaired; the intestinal health condition of the ricefield eel fed by the iced fresh fish can beobviously improved by mixing and feeding the preparation according to the proportion of 0.3%. The preparation is wide in raw material source, convenient and easy to purchase, easy to obtain and low in cost; the preparation is simple to prepare and operate without special instruments and equipment; and the preparation is simple and easy to use, high in applicability, free of residues after being used and environmentally friendly.

The invention discloses a dual-gRNA site amh gene knockout method in pelteobagrus fulvidraco. The method comprises the following steps of (1) designing a target site 1 on the first exon of a pelteobagrus fulvidraco amh gene, and designing a target site 2 on the fourth exon; (2) according to a target site point sequence design primer in the step (1), detecting the accuracy of the target site in parent fish, amplifying the target site 1 and a near sequence by using amh E1 F and amh E1 R, and amplifying the target site 2 and the near sequence by using amh E4 F and amh E4 R; (3) using a pUC19-gRNA-scaffold plasmid as a template, performing PCR amplification on a gRNA1 fragment by using amh E1 gRNA F and gRNA R, and performing PCR amplification on a gRNA2 fragment by using amh E4 gRNA F and gRNA R, wherein the PCR products are used as the template, in vitro transcription is performed, and purification is performed, so that gRNA is obtained; (4) using a pXT7-hCas9 linearization plasmid as atemplate, and performing in vitro transcription to synthetize Cas9 mRNA; (5) performing microinjection on Cas9 mRNA and two gRNA into a cell stage embryo of pelteobagrus fulvidraco; and (6) detectingthe mutation type, and calculating the gene editing rate. The invention further discloses an application of the method.

The invention discloses a plteobagrus fulvidraco feed for different breeding stages and application of the pelteobagrus fulvidraco feed. According to breeding scales, breeding time, market requirements and the like of pelteobagrus fulvidraco, pelteobagrus fulvidraco breeding stages are divided into a recovery stage, a rapid growth stage (also called as rapid growth early stage), a high temperaturestage, a new fish rapid growth stage (also called as rapid growth later stage) and a wintering period; and according to stage division of the plteobagrus fulvidraco, and together with research achievements of the plteobagrus fulvidraco, nutrition standards of the plteobagrus fulvidraco at different stages are confirmed, and the problems that stage feed standards are nor reasonably designed in breeding of the plteobagrus fulvidraco and nutrition standards are not matched with different specifications in breeding of the plteobagrus fulvidraco, can be solved.

The invention provides a feed additive for preventing and treating bacterial disease of pelteobagrus fulvidraco. The feed additive comprises the following raw materials in parts by weight of 20-26 parts of poria cocos, 16-24 parts of honeysuckle flowers, 25-35 parts of a dendrobium huoshanense extract, 6-12 parts of cortex eucommiae essential oil and 10-16 parts of a carrier, wherein the carrier is modified Maifan stone powder. The feed additive can effectively restrain growth of harmful pathogenic bacteria, and is notable in effects of preventing and treating bacterial gill diseases.

The invention discloses a feed for preventing pelteobagrus fulvidraco from body grayish white patches The feed comprises the following components of fish meal, hydrolyzed fish protein powder, soybeanprotein concentrate, spray-dried blood cell protein powder, beer yeast, meat and bone meal, fermented soybean meal, corn protein powder, seaweed meal, flour, corn, soybean oil, soybean phospholipid oil, monocalcium phosphate, vitamin C phosphate, vitamin A, vitamin D and a premix additive. The invention further discloses a preparation method and application of the feed at the same time. The feed can effectively enhance the immunity of the pelteobagrus fulvidraco, prevent the occurrence of body grayish white patch diseases or body rotting diseases, and also has a good treatment effect on the pelteobagrus fulvidraco suffering from the body grayish white patch diseases or the body rotting diseases; and meanwhile, the culture water environment can be improved, and livers and intestines of thepelteobagrus fulvidraco are protected, so that the culture survival rate is improved, and the culture benefits is increased.

The invention discloses a large-scale breeding method for hybrid pelteobagrus fulvidraco. The large-scale breeding method comprises the following steps: pond selection and cleaning: cleaning a pond, selecting the area and the trend of the breeding pond, cleaning the pond by mixing quick lime and water, removing sludge by using a bulldozer or sucking sludge by using a sludge pump, exposing the bottom of the pond to the sun, and drying the bottom of the pond to crack; building of fish nests: building multiple rows of fish nests in the pond through reeds or branches, arranging the multiple rows of fish nests on one side of the pond in the east-west direction and arranging aerators on bank sides; fry releasing: scattering fries, after the internal and external water temperatures are adapted, opening fry oxygen bags, and slightly scooping pond water into the fry oxygen bags; bait putting and guarantee of water quality. Compared with the prior art, the method has the advantages that multiple rows of fish nests are built in the pond, oxygen is increased through air pumps, and fry death caused by oxygen deficit during fry gathering can be prevented; and water in the pond is used for washing the outer surface of a foam box during fry releasing, so that the temperature difference inside and outside the oxygen bags of the foam box is balanced, the inside and outside water bodies are mixed and buffered, and the survival rate of fries is increased.

The invention discloses a special premix used for feed for preventing and treating the color change of pelteobagrus fulvidraco. The special premix comprises the following components in parts by weight: 30-40 parts of radix astragali, 10-20 parts of radix saposhnikoviae, 25-30 parts of rhizoma atractylodis macrocephalae, 5-10 parts of peanut coat, 30-40 parts of rhizoma coptidis, 7-10 parts of folium isatidis, 20-30 parts of compound vitamin, 1-5 parts of apple juice and 1-5 parts of garlic. According to the special premix, by adding the radix astragali, the radix saposhnikoviae and the rhizomaatractylodis macrocephalae, so that Yupingfeng powder of the traditional Chinese medicines is composed; as a representative of spleen and qi tonifying medicines, the Yupingfeng powder can greatly tonify qi of the spleen and the lung internally, and can consolidate superficial resistance externally, and thus, the physique of the pelteobagrus fulvidraco is improved; by adding the peanut coat, the effect of stopping bleeding can be enhanced, and the pelteobagrus fulvidraco can be effectively stopped from bleeding; and by adding the rhizoma coptidis, acute colitis can be treated, and the rhizomacoptidis has an anti-inflammatory effect, and can relieve inflammations of the pelteobagrus fulvidraco.

The invention discloses a pelteobagrus fulvidraco richardson mstna mutation allele and an application thereof. Compared with a pelteobagrus fulvidraco richardson mstna wild type allele, the pelteobagrus fulvidraco richardson mstna mutation allele disclosed by the invention has the differences that one protein with a truncated N terminal is estimated to be coded, and only first 42 amino acid residues are reserved. Pelteobagrus fulvidraco richardson carrying the mstna mutation allele disclosed by the invention can normally grow and propagate, male and female homozygote mutants all show obvious double muscling phenotypes, and the pelteobagrus fulvidraco richardson has the characteristics that when the elteobagrus fulvidraco richardson grow to one month, two obvious muscle lumps extend out between the head and the dorsal fin, and are obvious along with increment of ages. 80 and 210 days after fertilization, the weight of the pelteobagrus fulvidraco richardson of the mstna mutant is 1.27-1.37 times of that of wild pelteobagrus fulvidraco richardson. Histology analysis indicates that for the pelteobagrus fulvidraco richardson with a mutation mstna gene, disclosed by the invention, the number of muscle fibers for skeletal muscle is increased, the muscle fibers are small in shape and size, and the pelteobagrus fulvidraco richardson carrying the mutation allele disclosed by the invention can grow quickly and the muscle mass is high.