7 results about "Pelteobagrus eupogon" patented technology

The application discloses a method for promoting ovary development of Pelteobagrus vishnui, belongs to the technical field of fish culture, and feeds a functional additive during an ovary development preparation period and a strengthening period in the process of culturing Pelteobagrus vishnui, wherein the functional additive is a microcapsule prepared by mixing epigallocatechin gallate and a composite unsaturated fatty acid, embedding the inside layer by using beta-cyclodextrin, and coating the outside layer by using chitosan; and the composite unsaturated fatty acid is composed of monounsaturated fatty acid and polyunsaturated fatty acid at a volume ratio of 0.8-1:1-1.2. In the application, the EGCG and the composite unsaturated fatty acid are compounded, and then embedded to prepare the microcapsule, which is added into feed for feeding, so that the ovary development of Pelteobagrus vishnui is effectively promoted, the number of oocytes is increased, the proportion of III stage oocytes is increased, the fecundity of Pelteobagrus vishnui is effectively improved, and the spawning quantity, fertilization rate and hatching rate are increased.

The present application relates to the technical field of aquatic disease prevention and control, and particularly relates to a preparation method of a compound regulator for enteron inflammation of Pelteobagrus fulvidraco containing omega-3 fatty acid-lipoxin-phospholipid. The functional components of the compound regulator include eicosapentaenoic acid 20-30 parts, lipoxin B4 0.1-0.5 parts, phosphatidylserine 8-12 parts, dehydroabietic acid 1-2 parts, sophorobiose 10-18 parts and ethoxyquinoline 0.01-0.03 parts. The product integrates the functions of antibiosis, inflammation subsiding and tissue repair through a multi-target synergistic mechanism, and builds a complete inflammation regulation pathway. Meanwhile, the active ingredients are effectively protected by means of nano-liposome dosage form technology. The product realizes the antibiosis function by using natural ingredients. The technical scheme can solve the technical problems of low antibiosis efficiency, single mechanism and insufficient mucosal repair capacity of the existing prevention and control technology for enteron inflammation of Pelteobagrus fulvidraco, and has an ideal application prospect.

The invention belongs to the technical field of feed additives, and discloses a compound functional feed additive for pelteobagrus fulvidraco as well as a preparation method and application of the compound functional feed additive. The invention creatively provides a multi-target synergistic compound functional feed additive for pelteobagrus fulvidraco by scientifically compounding the antibacterial peptide, the eucommia ulmoides extract, the eucalyptus essential oil and the sodium butyrate and supplementing a carrier. The composite functional feed additive is not simply superposed in functions of all the components, but systematically improves the health level and disease resistance of the pelteobagrus fulvidraco from multiple aspects of pathogen inhibition, immunoregulation, oxidative stress resistance, intestinal barrier repair and the like through a synergistic effect, and can replace or reduce the use of antibiotics.

A method for promoting ovarian development of pelteobagrus vachelli belongs to the technical field of fish culture, and is characterized in that in the pelteobagrus vachelli culture process, a functional additive is fed at the ovarian development preparation stage and the ovarian development strengthening stage, and the functional additive is prepared by mixing epigallocatechin gallate and compound unsaturated fatty acid, adding the compound unsaturated fatty acid into the mixture, and stirring to obtain the functional additive for promoting the ovarian development of the pelteobagrus vachelli. Beta-cyclodextrin is firstly adopted for inner layer embedding, then chitosan is adopted for outer layer coating, the microcapsule is prepared, and the composite unsaturated fatty acid is composed of monounsaturated fatty acid and polyunsaturated fatty acid according to the volume ratio of (0.8-1): (1-1.2). EGCG and compound unsaturated fatty acid are compounded and embedded to prepare microcapsules, the microcapsules are added into feed for feeding, ovarian development of pelteobagrus vachelli is effectively promoted, the number of oocytes of pelteobagrus vachelli is increased, meanwhile, the cell proportion of III-stage oocytes is increased, the reproductive capacity of pelteobagrus vachelli is effectively improved, the survival rate of pelteobagrus vachelli is increased, and the survival rate of pelteobagrus vachelli is increased. The egg laying amount, the fertilization rate and the hatching rate are improved.

The present application relates to the field of biotechnology, and more particularly to a primer, kit and method for identifying Pelteobagrus vachelli, Pelteobagrus nitidus, Leiocassis longirostris and their hybrids, wherein the forward and reverse primer sequences in the primer are shown in SEQ ID NO: 1 and SEQ ID NO: 2, respectively. The present application uses fin strip micro samples for DNA extraction, and then completes species identification through PCR amplification combined with agarose gel electrophoresis technology. The detection result has high accuracy and strong genetic marker specificity, and is not subject to the growth of the fish body. The fish body can be sampled and identified at each breeding stage, thereby providing important technical support for aquatic breeding management.

The invention discloses a protein subunit vaccine adjuvant aiming at pathogenic bacteria of pelteobagrus fulvidraco, a preparation method and application, the protein subunit vaccine adjuvant is coded by a nucleotide sequence, the nucleotide sequence is shown as SEQ ID NO.1, the protein coded by the nucleotide sequence is the protein subunit vaccine adjuvant, and the protein subunit vaccine adjuvant is a protein subunit vaccine adjuvant. The protein subunit vaccine adjuvant comprises an amino acid as shown in SEQ ID NO. 2. The protein subunit vaccine adjuvant is pelteobagrus fulvidraco IL6 protein and can be used as a vaccine adjuvant, the immune effect of a pelteobagrus fulvidraco vaccine is remarkably improved, specifically, the activity of lysozyme in serum and the content of complement C3 are improved, the expression of immune-related genes (such as IL-1beta and TNF-alpha) is enhanced, and the protection rate of the vaccine on edwardsiella ictaluri after toxin attacking is remarkably increased. The invention further discloses a combined immune composition composed of the IL6 protein and the edwardsiella ictaluri OmpC protein, and application of the combined immune composition in preparation of edwardsiella ictaluri subunit vaccines.

The invention discloses an ecological restoration method suitable for turbidity reduction and purification of high-turbidity urban and rural rivers in plains. The ecological restoration method comprises the following steps: ecological water level regulation and control; bottom layer fishes and reptiles are removed; substrate improvement; submerged plants are planted at the substrate I; filter-feeding freshwater mussels are stocked at the substrate II; stocking filter-feeding fish silver carps and bighead carps; and stocking rhodeinae fishes and pelteobagrus fishes. According to the ecological restoration method suitable for turbidity reduction and purification of the high-turbidity urban and rural rivers in the plain, a new biocenosis can be established in the high-turbidity urban and rural rivers in the plain, the turbidity reduction and purification effects on the high-turbidity rivers can be achieved, and meanwhile the navigation, flood discharge and other effects of the rivers can be guaranteed; the method is very conveniently applicable to urban and rural high-turbidity rivers undertaking navigation and flood discharge effects, can realize organic unification of the ecological environment benefits of turbidity descending and purification and the economic and social benefits of the rivers, and has very good market popularization and use values.