21 results about "Pelteobagrus eupogon" patented technology

The invention discloses a Pelteobagrus vachelli population dynamic monitoring system and method, particularly relates to the technical field of fishery resource monitoring, and aims to solve the problems of inaccurate juvenile fish survival rate evaluation and poor monitoring timeliness in a benthic hidden environment in the prior art. The method comprises the following steps: arranging a benthic penetration type sonar array to synchronously collect a water body reflection signal and a benthic environment sample, and extracting juvenile fish specific gene fragments by combining an environment DNA (Deoxyribose Nucleic Acid) enrichment technology to generate concentration distribution data; screening juvenile fish distribution data by using an acoustic reflection signal and body length correlation model, and positioning an active hot spot area through space-time overlapping analysis; deploying a directional sound wave excitation device to obtain escape trajectory data, and calculating a survival rate in combination with a density attenuation model; finally, multi-source data and topographic features are fused to generate a real-time population dynamic monitoring result, and high-precision and non-intrusive monitoring of the juvenile fish population in the hidden environment is realized.

The present application discloses a specific DNA fragment for sex identification of Pelteobagrus ussuriensis and a method for sex identification, specifically relating to the technical field of sex identification of Pelteobagrus ussuriensis. The above-mentioned identification method includes the following steps: Dissect 5 female and 5 male Pelteobagrus ussuriensis of a full-sib family to determine the physiological sex; respectively extract DNA from the muscles of female samples and male samples, construct a paired-end genomic DNA library with an insert fragment size of 300 bp, and perform high-throughput sequencing; based on the GWAS and Fst methods, identify that the sex chromosome of Pelteobagrus ussuriensis is chromosome 8, and then screen for Indels within the sex determination region on chromosome 8; screen a 601-bp male-specific DNA sequence and design and synthesize primers; use PCR amplification and agarose gel electrophoresis detection, and the result without specific bands is female, and the one with a 356-bp band is male. This scheme has the advantages of strong specificity, simple primer composition, and more intuitive identification of the sex of Pelteobagrus ussuriensis.

The invention discloses a method for improving the survival rate of yellow catfish transportation, comprising the steps of: (1) selecting healthy yellow catfish fry to be put into aquaculture water tank, water filling in the water tank, then adding composite polysaccharide nanoparticles and composite probiotics, and the yellow catfish fry is temporarily kept in the tank for 1 3 days without food; (2) the yellow catfish fry temporarily kept without food by water and step (1) are packed in aquatic product special oxygen bag, immediately oxygenating and sealing and ensuring that oxygen content is 6 7mg / L, and the bag is put into the carton of inner foam for transportation; the composite polysaccharide nanoparticles include chitosan, astragalus polysaccharide, oligoxylose, roxburghii polysaccharide and wolfberry polysaccharide and are made. The composite polysaccharide nanoparticles of the present invention are conducive to the absorption of polysaccharide and act on tissue, increase water solubility, dispersibility, and the absorption of fish body thereto, effectively avoid the side effect of polysaccharide accumulation and pollute water body, reduce the time and economic cost added to the feed, thus realize the relief of the fish stress under transportation stress.

The application discloses a method for promoting ovary development of Pelteobagrus vishnui, belongs to the technical field of fish culture, and feeds a functional additive during an ovary development preparation period and a strengthening period in the process of culturing Pelteobagrus vishnui, wherein the functional additive is a microcapsule prepared by mixing epigallocatechin gallate and a composite unsaturated fatty acid, embedding the inside layer by using beta-cyclodextrin, and coating the outside layer by using chitosan; and the composite unsaturated fatty acid is composed of monounsaturated fatty acid and polyunsaturated fatty acid at a volume ratio of 0.8-1:1-1.2. In the application, the EGCG and the composite unsaturated fatty acid are compounded, and then embedded to prepare the microcapsule, which is added into feed for feeding, so that the ovary development of Pelteobagrus vishnui is effectively promoted, the number of oocytes is increased, the proportion of III stage oocytes is increased, the fecundity of Pelteobagrus vishnui is effectively improved, and the spawning quantity, fertilization rate and hatching rate are increased.

The present invention discloses a system and method for monitoring the dynamics of yellow catfish populations in yellow catfish (Psoralea vachelli), which specifically relate to the technical field of fishery resource monitoring. The system and method are used to solve the problems of inaccurate juvenile survival rate assessment and poor monitoring timeliness in benthic hidden environments in the prior art. The system deploys a benthic penetrating sonar array to synchronously collect water body reflection signals and benthic environment samples, and uses environmental DNA enrichment technology to extract juvenile-specific gene fragments to generate concentration distribution data. The system uses an acoustic reflection signal and body length correlation model to screen juvenile distribution data, and locates activity hotspots through spatiotemporal overlap analysis. A directional acoustic wave excitation device is deployed to obtain escape trajectory data, and the survival rate is calculated using a density attenuation model. Finally, multi-source data and terrain features are integrated to generate real-time population dynamics monitoring results, thereby achieving high-precision, non-invasive monitoring of juvenile fish populations in hidden environments.

The invention relates to the technical field of prevention and control of aquatic diseases, in particular to a preparation method of a pelteobagrus fulvidraco intestinal inflammation composite regulation preparation containing omega-3 fatty acid-lipoxin-phospholipid. The composite regulation and control preparation is prepared from the following functional components: 20 to 30 parts of eicosapentaenoic acid, 0.1 to 0.5 part of lipoxin B4, 8 to 12 parts of phosphatidylserine, 1 to 2 parts of dehydroabietic acid, 10 to 18 parts of sophorobiose and 0.01 to 0.03 part of ethoxyquin. The product integrates the functions of resisting bacteria, promoting inflammation regression and promoting tissue repair through a multi-target synergistic mechanism to construct a complete inflammation regulation and control pathway; meanwhile, by virtue of a nano-liposome dosage form technology, the active ingredients are effectively protected; the product is completely compounded from natural components to realize an antibacterial effect. According to the technical scheme, the technical problems of low antibacterial efficiency, single action mechanism, insufficient mucous membrane repair capability and the like of the existing prevention and control technology for the intestinal inflammation of the pelteobagrus fulvidraco can be solved, and the pelteobagrus fulvidraco has an ideal application prospect.

The invention discloses an intestinal microbial marker combination and system for detecting fish liver injury and application, and belongs to the technical field of molecular detection of fish liver injury. The invention relates to an enteric microbial marker combination, which is characterized in that the enteric microbial marker combination comprises the following components: cetyl, pseudomonas and Escherichia-Shigella, and the enteric microbial marker combination comprises the following components: bacillus, pseudomonas and Escherichia-Shigella, and the enteric microbial marker combination comprises the following components: bacillus, pseudomonas and Escherichia-Shigella. By utilizing the intestinal microbial marker combination, non-invasive diagnosis of liver injury can be realized on the premise of not invading a fish body, the accuracy is high, missing detection is avoided, and particularly, the condition that liver injury is misjudged to cause excessive treatment is avoided. A judgment basis is provided for breeding environment regulation and control and precise disease prevention and treatment, the breeding survival rate of the pelteobagrus fulvidraco is increased, and the breeding benefit is improved.

The present application relates to the technical field of aquatic disease prevention and control, and particularly relates to a preparation method of a compound regulator for enteron inflammation of Pelteobagrus fulvidraco containing omega-3 fatty acid-lipoxin-phospholipid. The functional components of the compound regulator include eicosapentaenoic acid 20-30 parts, lipoxin B4 0.1-0.5 parts, phosphatidylserine 8-12 parts, dehydroabietic acid 1-2 parts, sophorobiose 10-18 parts and ethoxyquinoline 0.01-0.03 parts. The product integrates the functions of antibiosis, inflammation subsiding and tissue repair through a multi-target synergistic mechanism, and builds a complete inflammation regulation pathway. Meanwhile, the active ingredients are effectively protected by means of nano-liposome dosage form technology. The product realizes the antibiosis function by using natural ingredients. The technical scheme can solve the technical problems of low antibiosis efficiency, single mechanism and insufficient mucosal repair capacity of the existing prevention and control technology for enteron inflammation of Pelteobagrus fulvidraco, and has an ideal application prospect.

The invention provides a pelteobagrus fulvidraco embryonic cell line as well as a subculture method and application thereof, and belongs to the field of fish cell biology. The pelteobagrus fulvidraco cell line is a pelteobagrus fulvidraco embryo-derived cell line and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: C202516. The pelteobagrus fulvidraco embryonic cell line YCE is a fibrous cell, is continuously cultured for more than 100 generations, has a good growth state, can be stably passaged, can provide a large number of pelteobagrus fulvidraco embryonic cells, can be cryopreserved, and can still be stably passaged after being unfrozen. The fish embryo source cell line disclosed by the invention not only can be used for related research on an anti-infection immune mechanism of pelteobagrus fulvidraco, but also provides a new thought for an establishment method of a freshwater fish embryo cell line, and enriches basic biological data of fishes.

The utility model relates to the technical field of pelteobagrus fulvidraco, and provides novel pelteobagrus fulvidraco hatching equipment which comprises a hatching box, a water body exchange assembly is arranged at the left end of the hatching box and used for replacing a water body for hatching pelteobagrus fulvidraco, the right end of the hatching box is fixedly connected with a rotating seat, and the inner wall of the left end of the rotating seat is rotationally connected with a box door through a rotating shaft. A monitoring assembly is arranged at the center of the outer wall of the bottom end of the box door and used for monitoring the incubation condition of pelteobagrus fulvidraco, a plurality of irradiation lamps are fixedly connected to the outer wall of the bottom end of the box door and annularly arrayed at the bottom end of the box door, and a fish conveying assembly is arranged at the bottom end of the interior of the incubation box and used for conveying pelteobagrus fulvidraco cubs. When unforeseen circumstances occur, corresponding solutions can be taken in time, meanwhile, a clean water source is provided for incubation of cubs through the water purification box, it is avoided that mold is bred in the water, growth of juvenile fishes is affected, and then the incubation efficiency of the device is improved.

The invention belongs to the technical field of feed additives, and discloses a compound functional feed additive for pelteobagrus fulvidraco as well as a preparation method and application of the compound functional feed additive. The invention creatively provides a multi-target synergistic compound functional feed additive for pelteobagrus fulvidraco by scientifically compounding the antibacterial peptide, the eucommia ulmoides extract, the eucalyptus essential oil and the sodium butyrate and supplementing a carrier. The composite functional feed additive is not simply superposed in functions of all the components, but systematically improves the health level and disease resistance of the pelteobagrus fulvidraco from multiple aspects of pathogen inhibition, immunoregulation, oxidative stress resistance, intestinal barrier repair and the like through a synergistic effect, and can replace or reduce the use of antibiotics.

A method for promoting ovarian development of pelteobagrus vachelli belongs to the technical field of fish culture, and is characterized in that in the pelteobagrus vachelli culture process, a functional additive is fed at the ovarian development preparation stage and the ovarian development strengthening stage, and the functional additive is prepared by mixing epigallocatechin gallate and compound unsaturated fatty acid, adding the compound unsaturated fatty acid into the mixture, and stirring to obtain the functional additive for promoting the ovarian development of the pelteobagrus vachelli. Beta-cyclodextrin is firstly adopted for inner layer embedding, then chitosan is adopted for outer layer coating, the microcapsule is prepared, and the composite unsaturated fatty acid is composed of monounsaturated fatty acid and polyunsaturated fatty acid according to the volume ratio of (0.8-1): (1-1.2). EGCG and compound unsaturated fatty acid are compounded and embedded to prepare microcapsules, the microcapsules are added into feed for feeding, ovarian development of pelteobagrus vachelli is effectively promoted, the number of oocytes of pelteobagrus vachelli is increased, meanwhile, the cell proportion of III-stage oocytes is increased, the reproductive capacity of pelteobagrus vachelli is effectively improved, the survival rate of pelteobagrus vachelli is increased, and the survival rate of pelteobagrus vachelli is increased. The egg laying amount, the fertilization rate and the hatching rate are improved.

The invention discloses a pelteobagrus fulvidraco calicivirus and streptococcus iniae dual fluorescent quantitative PCR method, and belongs to the technical field of biological diagnosis. The invention provides a pelteobagrus fulvidraco calicivirus and streptococcus iniae dual detection primer and a fluorescent probe. The pelteobagrus fulvidraco calicivirus and streptococcus iniae dual detection primer comprises primers as shown in SEQ ID NO.1-2 and SEQ ID NO.4-5, and the fluorescent probe comprises fluorescent probes as shown in SEQ ID NO.3 and SEQ ID NO.6. According to the present invention, the rapid and accurate diagnosis of the two pathogens is achieved by amplifying and detecting the two nucleic acids such as the pelteobagrus fulvidraco calicivirus and the streptococcus iniae by using the principle of the fluorescent quantitative PCR technology, and the lowest detection limits of the YcCV and the S. iniae are respectively 1 * 100 copies / [mu] L and 1 * 100 copies / [mu] L by using the detection method provided by the present invention. Important technical support is provided for prevention and control of diseases of the pelteobagrus fulvidraco.

The application belongs to the field of monoclonal antibodies, and particularly relates to a mouse anti-Pelteobagrus nigro-maculatus IgM monoclonal antibody, a hybridoma cell strain secreting the monoclonal antibody and application, the CDR region of the heavy chain variable region of the mouse anti-Pelteobagrus nigro-maculatus IgM monoclonal antibody comprises three sequences as shown in SEQ ID NO. 1~3, the CDR region of the light chain variable region comprises three sequences as shown in SEQ ID NO. 4~6; the mouse anti-Pelteobagrus nigro-maculatus IgM monoclonal antibody can be obtained by the hybridoma cell strain PFM-1B7C12A1B2 with the preservation number of CCTCC NO: C2024234. The mouse anti-Pelteobagrus nigro-maculatus IgM monoclonal antibody provided by the application can be used for Western blot and ELISA specific detection of secreted IgM of Pelteobagrus nigro-maculatus, and can be used for flow cytometry identification of Pelteobagrus nigro-maculatus IgM + B cells. The monoclonal antibody has good specificity, high sensitivity and strong affinity, and can be used for structural analysis of Pelteobagrus nigro-maculatus IgM, detection of immune response level and the like, thereby laying a foundation for in-depth research on the immune system of Pelteobagrus nigro-maculatus and establishment of a vaccine immune effect evaluation method taking an antibody level as an index.

The present application relates to the field of biotechnology, and more particularly to a primer, kit and method for identifying Pelteobagrus vachelli, Pelteobagrus nitidus, Leiocassis longirostris and their hybrids, wherein the forward and reverse primer sequences in the primer are shown in SEQ ID NO: 1 and SEQ ID NO: 2, respectively. The present application uses fin strip micro samples for DNA extraction, and then completes species identification through PCR amplification combined with agarose gel electrophoresis technology. The detection result has high accuracy and strong genetic marker specificity, and is not subject to the growth of the fish body. The fish body can be sampled and identified at each breeding stage, thereby providing important technical support for aquatic breeding management.

The invention discloses a protein subunit vaccine adjuvant aiming at pathogenic bacteria of pelteobagrus fulvidraco, a preparation method and application, the protein subunit vaccine adjuvant is coded by a nucleotide sequence, the nucleotide sequence is shown as SEQ ID NO.1, the protein coded by the nucleotide sequence is the protein subunit vaccine adjuvant, and the protein subunit vaccine adjuvant is a protein subunit vaccine adjuvant. The protein subunit vaccine adjuvant comprises an amino acid as shown in SEQ ID NO. 2. The protein subunit vaccine adjuvant is pelteobagrus fulvidraco IL6 protein and can be used as a vaccine adjuvant, the immune effect of a pelteobagrus fulvidraco vaccine is remarkably improved, specifically, the activity of lysozyme in serum and the content of complement C3 are improved, the expression of immune-related genes (such as IL-1beta and TNF-alpha) is enhanced, and the protection rate of the vaccine on edwardsiella ictaluri after toxin attacking is remarkably increased. The invention further discloses a combined immune composition composed of the IL6 protein and the edwardsiella ictaluri OmpC protein, and application of the combined immune composition in preparation of edwardsiella ictaluri subunit vaccines.

The invention belongs to the technical field of molecular marker assisted breeding, and particularly relates to application and a method of an SNP molecular marker in pelteobagrus fulvidraco coarse feed utilization character detection or breeding. The SNP molecular marker is located at the 228bp position of a nucleotide sequence shown as SEQ ID NO.4 and has TT, TG and GG genotypes, and the SNP molecular marker is located at the 228bp position of the nucleotide sequence shown as SEQ ID NO.3. The roughage utilization character of the Pelteobagrus fulvidraco individual of the GG genotype is obviously superior to that of the Pelteobagrus fulvidraco individual of the TT genotype and the By detecting the genotype of the molecular marker, rapid, accurate and high-throughput identification or detection of the utilization character of the pelteobagrus fulvidraco roughage on the molecular level can be realized, and the seed selection period of a pelteobagrus fulvidraco strain with high roughage utilization rate and excellent roughage tolerance is greatly shortened; the cultivation of the pelteobagrus fulvidraco group which can efficiently utilize the coarse feed and has the rapid growth character under the feeding of the coarse feed can be obviously accelerated.

The invention discloses an ecological restoration method suitable for turbidity reduction and purification of high-turbidity urban and rural rivers in plains. The ecological restoration method comprises the following steps: ecological water level regulation and control; bottom layer fishes and reptiles are removed; substrate improvement; submerged plants are planted at the substrate I; filter-feeding freshwater mussels are stocked at the substrate II; stocking filter-feeding fish silver carps and bighead carps; and stocking rhodeinae fishes and pelteobagrus fishes. According to the ecological restoration method suitable for turbidity reduction and purification of the high-turbidity urban and rural rivers in the plain, a new biocenosis can be established in the high-turbidity urban and rural rivers in the plain, the turbidity reduction and purification effects on the high-turbidity rivers can be achieved, and meanwhile the navigation, flood discharge and other effects of the rivers can be guaranteed; the method is very conveniently applicable to urban and rural high-turbidity rivers undertaking navigation and flood discharge effects, can realize organic unification of the ecological environment benefits of turbidity descending and purification and the economic and social benefits of the rivers, and has very good market popularization and use values.

The invention belongs to the technical field of body color stabilization of freshwater fishes in cold-chain transportation and storage. The invention relates to a body color protection method in cold-chain transportation and storage of pelteobagrus fulvidraco based on a color protection preservative. The body color protection method is characterized by comprising the following steps: 1) preparing a color protection preservative solution; 2) preparing a body surface protective film solution; (3) temporarily rearing fresh pelteobagrus fulvidraco for 6 hours to relieve transportation stress, stirring in an ice-water bath, slaughtering, removing viscera and gills, cleaning, and simply draining; soaking the drained pelteobagrus fulvidraco in the color-protecting preservative solution obtained in the step (1) for 3 hours; then uniformly spraying the body surface protective film solution prepared in the step 2) on the surface of the fish body; (4) putting the pelteobagrus fulvidraco subjected to vacuum packaging in the step (3) into a quick-freezing warehouse for quick-freezing; (5) in the transportation and storage stages, proper environmental conditions are kept; and 6) taking out the quick-frozen pelteobagrus fulvidraco from the vacuum bag, and directly thawing the pelteobagrus fulvidraco together with the protective film on the surface in a running water thawing manner. The method is remarkable and stable in color protection effect.

The invention relates to the technical field of biology, in particular to primers, a kit and a method for identifying pelteobagrus fulvidraco, pelteobagrus vachelli, leiocassis longirostris and hybrids of the pelteobagrus fulvidraco, the pelteobagrus vachelli, the leiocassis longirostris and the hybrids of the pelteobagrus fulvidraco and the leiocassis longirostris. According to the method, after DNA extraction is carried out on a fin ray trace sample, species identification can be completed through combination of PCR amplification and an agarose gel electrophoresis technology, the accuracy of a detection result is high, the genetic marker specificity is high, the method is not limited by the growth condition of fish bodies, sampling identification can be carried out in all breeding stages, and an important technical support is provided for aquatic product breeding management.

The invention discloses a specific DNA fragment for sex determination of pelteobagrus fulvidraco and a sex determination method, and particularly relates to the technical field of sex determination of pelteobagrus fulvidraco. The identification method comprises the following steps: dissecting five female pelteobagrus fulvidraco and five male pelteobagrus fulvidraco of a full-sib family to determine the physiological gender; the method comprises the following steps: respectively taking muscles of a female sample and a male sample, carrying out DNA extraction, constructing a double-terminal genome DNA library with the insertion fragment size of 300bp, and carrying out high-throughput sequencing; the method comprises the following steps: identifying the sex chromosome of the pelteobagrus fulvidraco as a chromosome 8 based on GWAS and Fst methods, and then carrying out Indel screening in a sex determination region on the chromosome 8; a male specific DNA sequence of 601bp is screened out, and primer design and synthesis are carried out; pCR (polymerase chain reaction) amplification and agarose gel electrophoresis are utilized for detection, and the result shows that the female is the female and the male is the male with a 356bp band without a specific band. The scheme has the advantages that the specificity is high, the primer composition is simple, and the sex of the pelteobagrus fulvidraco can be more intuitively identified.