An affinity peptide for detecting human cancer cells and its application

A cancer cell and polypeptide technology, applied in the field of affinity peptide detection of human cancer cells, can solve the problems of complicated technology, high rate of misdiagnosis and missed diagnosis, and long time consuming.

Active Publication Date: 2020-07-24
INST OF CHEM CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Early diagnosis and treatment of malignant tumors can significantly improve patient survival and cure rates, but the current general diagnostic methods have limitations such as complicated techniques, high rates of misdiagnosis and missed diagnosis, and long time-consuming, so early diagnosis is still a key problem in this field.

Method used

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  • An affinity peptide for detecting human cancer cells and its application
  • An affinity peptide for detecting human cancer cells and its application
  • An affinity peptide for detecting human cancer cells and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1. Detection of affinity peptides for human cancer cells

[0104] 1. Design of affinity peptide

[0105] After a lot of pre-experiments and functional verification, a group of functional polypeptides that specifically bind to the first extracellular region of the LAPTM4B protein in human liver cancer and other cancer cells (EL1 for short) were designed, named AP1, AP2, AP3, and AP4 respectively. , AP5, AP6, AP7 and AP8, EL1 and the sequences of eight functional polypeptides are shown in Table 1.

[0106] The letters represent amino acids as follows: alanine A, aspartic acid D, proline P, glutamine Q, tyrosine Y, asparagine N, phenylalanine F, serine S, glutamic acid E, Leucine L, Glycine G, Isoleucine I, Arginine R, Valine V, Lysine K, Threonine T.

[0107] Table 1. EL1 sequence and polypeptide sequence

[0108]

[0109]

[0110] 2. Preparation of fluorescent probes for peptide screening

[0111] Use the FMOC solid-phase peptide synthesis method to syn...

Embodiment 2

[0151] Example 2, Application of Affinity Peptides in Detecting Human Cancer Cells

[0152] 1. Preparation of AP2-Cy7 and AP5-Cy7 conjugates

[0153] 1. Dissolve 100mg of IR-780 iodide and 18mg of 3-azidopropylamine in 15mL of DMF, react at 80°C for 16 hours, distill off the solvent under reduced pressure, and purify the residue by silica gel column chromatography (dichloromethane / methanol, 20:1 , v / v), to obtain 47mg dark blue solid, which is the azide Cy7. The structural formula and mass spectrometric identification results of azide Cy7 are shown in Figure 4 .

[0154] 2. Polypeptides AP2 and AP5 with alkyne groups at the end were directly synthesized on Wang resin by FMOC solid-phase peptide synthesis method. The specific method is: start to synthesize the polypeptide sequence from the C-terminus, and finally connect propargylglycine to the N-terminus. After the Kaiser reagent detects that the reaction is complete, the terminal FMOC protecting group is removed, and then...

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Abstract

The invention discloses an affinity peptide for detecting human cancer cells, and applications thereof, and provides a polypeptide, which can meet the following general formula (a1) or (a2): (a1) T-S-Q-F-X-X-X-K-V-I-L-I-R-I-S, and (a2) T-S-Q-F-R-T-X-K-V-I-L-I-R-I-S, wherein X is any amino acid residue. The invention further provides a conjugate obtained by conjugating the polypeptide to other materials. According to the present invention, the polypeptide has the short sequence, is easily subjected to large-scale production, has no toxicity to cells, can overcome the disadvantages of cumbersomepreparation, poor stability, high cost, easy immune reaction generation, weak penetration and the like of antibodies and other biological preparations, and can be used as the diagnosis reagent for human liver cancer and other cancers.

Description

technical field [0001] The invention relates to an affinity peptide for detecting human cancer cells and its application. Background technique [0002] Malignant tumors seriously threaten human health. Because its pathogenesis has not been fully elucidated, there have been problems such as difficulty in early diagnosis, few innovative drugs at the source, and lack of effective treatment methods. Early diagnosis and treatment of malignant tumors can significantly improve patient survival and cure rates. However, the current general diagnostic methods have limitations such as complicated techniques, high misdiagnosis and missed diagnosis rates, and long time-consuming. Therefore, early diagnosis is still a key problem in this field that needs to be tackled urgently. If we can develop a fast and sensitive new detection method for the unique biomarkers of cancer cells, and develop a simple and effective early diagnosis tool for cancer with low side effects, it will be of great ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08G01N33/68G01N33/574
CPCC07K7/08G01N33/574G01N33/57438G01N33/68G01N2410/00
Inventor 赵睿何佳媛黄嫣嫣桂诗浪金钰龙
Owner INST OF CHEM CHINESE ACAD OF SCI
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