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Cumulus cell recognition and sorting device

A sorting device and cumulus cell technology, which is applied in the field of assisted reproduction, can solve the problems of stringent requirements, cumulus cell sensitivity, and high missed detection rate of manual cumulus cells, so as to reduce operating costs and improve accuracy. Effect

Active Publication Date: 2019-03-01
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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Problems solved by technology

[0004] Manual collection of cumulus cells has many disadvantages: First, due to the complex composition of follicular fluid and the very sensitive and fragile cumulus cells, there is a high probability of missed detection, which requires experienced operators
[0005] From the above disadvantages, we can see that the manual extraction of cumulus cells not only has strict requirements on the operator, but also causes great damage to the cumulus cells.
The missed detection rate of manual extraction of cumulus cells is also high
At present, there is still a lack of excellent methods to solve the above problems

Method used

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Embodiment Construction

[0034] The following are preferred embodiments of the present invention. It should be pointed out that for those skilled in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications are also considered as the present invention. protection scope of the invention.

[0035] Please refer to figure 1, the embodiment of the present invention provides a cumulus cell identification and sorting device, including a fluid system 1, an imaging system 3 and a control system 2; the fluid system 1 includes an ovum retrieval needle 11, a main input tube 12, a cell Sorting device 13 , cell buffer device 16 , waste liquid buffer device 15 , main output pipe 20 , peristaltic pump 22 , waste liquid tank 23 , and the egg retrieval needle 11 is used to obtain follicular fluid. The cell sorting device is used 13 to sort out the cumulus cells. The cell buffer device 16 is used to temporarily...

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Abstract

The invention provides a cumulus cell recognition and sorting device. The cumulus cell recognition and sorting device comprises a liquid road system, an imaging system and a control system; and the liquid road system comprises an ovum taking needle, a main input tube, a cell sorting device, a cell buffering device, a waste liquid buffering device, a main output tube, a peristaltic pump and a wasteliquid box. An inlet of the cell sorting device is connected with the ovum taking needle through the main input tube, a first outlet of the cell sorting device is connected with the cell buffering device through a cumulus cell inlet tube, and a second outlet of the cell sorting device is connected with the waste liquid buffering device through a waste inlet tube. The main output tube is connectedwith the cell buffering device through a first waste liquid outlet tube and is connected with the waste liquid buffering device through a second waste liquid outlet tube, a three-way valve is arranged between first waste liquid outlet tube, the second waste liquid outlet tube and a end opening adjacent to the main output tube, and the main output tube is sequentially connected with the peristaltic pump and the waste liquid box. By using the cumulus cell recognition and sorting device, cumulus cells can be automatically recognized and sorted, and the accuracy of recognizing and sorting the cumulus cells is improved.

Description

technical field [0001] The invention belongs to the technical field of assisted reproduction, and in particular relates to a device for identifying and sorting cumulus cells. Background technique [0002] The collection of cumulus cells plays an important role in the field of assisted reproductive technology and plays an important role in the process of in vitro fertilization. Therefore, how to improve the recovery rate of cumulus cells has become the development direction of assisted reproductive technology. [0003] At present, the collection of cumulus cells mainly adopts the puncture and oocyte retrieval technology guided by transvaginal ultrasound imaging. The detailed process is as follows: (1) Egg retrieval process: the egg retrieval needle is used to puncture the follicle through the vagina under the guidance of the ultrasound probe, and the follicle fluid containing cumulus cells is sucked into the test tube by negative pressure suction. If the test tube is full, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/26
CPCC12M21/06C12M33/00C12M33/04C12M41/24C12M41/40
Inventor 吴天准蒋伯石
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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