Application of inhibitor in preparation of drugs for treating cystic echinococcosis

A technology of cystic echinococcosis and inhibitors, which is applied in the field of medicine and can solve the problems that research has not yet been carried out

Active Publication Date: 2019-03-05
THE FIRST TEACHING HOSPITAL OF XINJIANG MEDICAL UNIVERCITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have shown that there is high or abnormal expression of EGFR in many solid tumors, so as to achie

Method used

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  • Application of inhibitor in preparation of drugs for treating cystic echinococcosis
  • Application of inhibitor in preparation of drugs for treating cystic echinococcosis
  • Application of inhibitor in preparation of drugs for treating cystic echinococcosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] The pharmacodynamic experiment of embodiment 1 drug screening

[0070] 1. Experimental content

[0071] 1.1 In vitro killing of Echinococcus granulosus protoscolum

[0072] Collection of protocercariae: livers of sheep with hepatic echinococcosis were purchased from the Urumqi slaughterhouse and brought back to the laboratory. First wash the blood attached to the surface of the sheep liver with water, and then use scissors to remove the fat tissue on the surface of the sheep liver as much as possible. Wipe the surface of the sheep liver with 75% alcohol, draw out the cyst fluid in the cyst with a 50ml syringe, put it in a 50ml centrifuge tube, and then use ophthalmic scissors to cut a visual window of about 2cm along the joint between the cyst and the liver tissue. Carefully peel off the inner capsule of the cyst with tweezers, and place it in a petri dish filled with physiological saline. Collect the protoscoleia from the natural sedimentation in the cyst fluid and ...

Embodiment 2

[0084] The in vitro killing of embodiment 2 encapsulation (Degrasyn)

[0085] Inject the protoscoleum directly into the peritoneal cavity, hepatic portal vein or liver parenchyma of the rodent intermediate animal, or inject the transformed cyst of the protoscoleum cultured in vitro into the rodent intermediate animal. The mice were dissected and the cysts were removed. Sterile and intact cysts were dispensed into 6-well plates and various concentrations of Degrasyn were added. Dynamically observe whether the cyst is ruptured, collapsed, and the germinal layer falls off within a week to determine the in vitro curative effect of the drug.

[0086]Cysts with a diameter ranging from 5mm to 9mm were taken, and the concentration was 20ug / ml in the experiment. The mortality rate of the cysts in the albendazole group was 0%, while that of Degrasyn was as high as 60%. The in vitro experiments of encapsulation show that Degrasyn has a better effect on killing encapsulation than albend...

Embodiment 3

[0087] The in vitro killing of embodiment 3 encapsulation (Tyrphostin9)

[0088] Inject the protoscoleum directly into the peritoneal cavity, hepatic portal vein or liver parenchyma of the rodent intermediate animal, or inject the transformed cyst of the protoscoleum cultured in vitro into the rodent intermediate animal. The mice were dissected and the cysts were removed. Sterile and complete capsules (diameter ranging from 5 mm to 10 mm) were distributed to 6-well plates, and Tyrphostin9 was added with different concentrations. Dynamically observe whether the cyst is ruptured, collapsed, and the germinal layer falls off within a week to determine the in vitro curative effect of the drug.

[0089] Experimental observations showed that when the drug concentration was 20ug / ml, the cyst mortality rate in the Tyrphostin9 group was 60%, while that in the albendazole group was only 0%. It shows that Tyrphostin9 is better than albendazole in killing cysts in vitro.

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Abstract

The invention relates to application of an inhibitor in preparation of drugs for treating cystic echinococcosis, and particularly discloses application of an inhibitor for preparing drugs for treatingcystic echinococcosis. The inhibitor is selected from the following inhibitors: a deubiquitinating enzyme inhibitor, an EGFR inhibitor or a combination thereof. The inhibitor can effectively kill echinococcus granulosus (particularly echinococcus granulosus larva), and then the cystic echinococcosis is treated.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the use of an inhibitor in the preparation of medicines for treating cystic echinococcosis. Background technique [0002] Cystic echinococcosis (CE) and alveolar echinococcosis ( Alveolare chinococcosis, AE). The high-incidence areas of echinococcosis in my country are mainly prevalent in alpine meadow areas, pastoral areas with cold climate, drought and little rain, and semi-agricultural and semi-pastoral areas. Xinjiang, Tibet, Gansu, Ningxia and other areas are more serious, and Xinjiang is one of the high-incidence areas of echinococcosis. one. [0003] Dogs and foxes often feed on rodents, and rodents can easily eat vegetation polluted by the feces and eggs of foxes and other animals, so that their life cycle can be completed. After foxes swallowed rodents or animal organs with alveolar coccoids, protoscoleia developed into adults after about 45 days, and there were gesta...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K31/44A61K31/517A61K31/275A61K31/47A61K31/506A61P33/10
CPCA61K31/275A61K31/44A61K31/47A61K31/506A61K31/517A61K45/00A61K2300/00
Inventor 张文宝李军王甜郑雪婷段李平温浩
Owner THE FIRST TEACHING HOSPITAL OF XINJIANG MEDICAL UNIVERCITY
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