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Kit for preparing umbilical cord mesenchymal stem cells

A quality stem cell and kit technology, applied in the field of stem cells, can solve the problems of donor pain, bleeding, chronic pain, infection, etc., and achieve the effects of convenient use, no serum, and low cost.

Inactive Publication Date: 2019-03-05
QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the number and proliferation ability of stem cells decrease significantly with age, the number and activity of mesenchymal stem cells derived from bone marrow and fat are affected to a certain extent, and the acquisition of bone marrow and fat is invasive, causing pain to the donor, and may also lead to infection, bleeding, and chronic pain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Preparation and application of a kit for preparing umbilical cord mesenchymal stem cells

[0020] 1. Preparation of kits for preparing umbilical cord mesenchymal stem cells

[0021] 1. Solution configuration

[0022] The kit for preparing umbilical cord mesenchymal stem cells of the present invention includes the following reagents:

[0023] (1) Configuration method of umbilical cord preservation solution: penicillin (brand Amresco, product number 0242) 200mg, streptomycin (brand Amresco, product number 0382) 200mg, amphotericin (brand Amresco, product number E437) 5mg, progesterone (Sigma, V900699- 5g) 2μg, insulin (Sigma, I0908) 10mg, vitamin C (Sigma, A5960-10mg) 2mmol, dissolved in 1000ml DMEM medium and sterilized by 0.22um filter.

[0024] (2) Configuration method of umbilical cord washing solution: 200mg of penicillin, 200mg of streptomycin, 5mg of amphotericin, 2μg of progesterone, 10mg of insulin, 2mmol of vitamin C, dissolved in 1000ml of normal sa...

Embodiment 2

[0047] Example 2 Preparation and application of the kit for preparing umbilical cord mesenchymal stem cells

[0048] 1. Preparation of kits for preparing umbilical cord mesenchymal stem cells

[0049] 1. Solution configuration

[0050] The kit for preparing umbilical cord mesenchymal stem cells of the present invention includes the following reagents:

[0051] (1) Configuration method of umbilical cord preservation solution: penicillin 100mg, streptomycin 100mg, amphotericin 2.5mg, progesterone 3μg, insulin 30mg, vitamin C 4mmol, dissolved in 1000ml DMEM / F12 medium 0.22um filter sterilization Can.

[0052] (2) Configuration method of umbilical cord washing solution: 100mg of penicillin, 100mg of streptomycin, 2.5mg of amphotericin, 3μg of progesterone, 30mg of insulin, 4mmol of vitamin C, dissolved in 1000ml of normal saline 0.22um, filtered and sterilized.

[0053] (3) Configuration method of umbilical cord decomposition solution: dissolve 2 g of collagenase II, 0.2 g of d...

Embodiment 3

[0063] Example 3 Preparation and application of a kit for preparing umbilical cord mesenchymal stem cells

[0064] 1. Preparation of kits for preparing umbilical cord mesenchymal stem cells

[0065] 1. Solution configuration

[0066] The kit for preparing umbilical cord mesenchymal stem cells of the present invention includes the following reagents:

[0067] (1) Configuration method of umbilical cord preservation solution: 150mg of penicillin, 150mg of streptomycin, 2.5mg of amphotericin, 2.5μg of progesterone, 25mg of insulin, 3mmol of vitamin C, dissolved in 1000ml of DMEM medium, 0.22um filter and sterilized. .

[0068] (2) Configuration method of umbilical cord washing solution: 150mg of penicillin, 150mg of streptomycin, 2.5mg of amphotericin, 2.5μg of progesterone, 25mg of insulin, 3mmol of vitamin C, dissolved in 1000ml of normal saline, 0.22um and filtered to sterilize.

[0069] (3) Configuration method of umbilical cord decomposition solution: dissolve 2 g of colla...

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PUM

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Abstract

The invention discloses a kit for preparing umbilical cord mesenchymal stem cells, and the kit is characterized in that the kit comprises an umbilical cord storage solution, an umbilical cord washingsolution, an umbilical cord decomposition solution, a cell washing solution, a cell culture solution, a cell digestion solution and a cell cryopreservation solution which are respectively bottled andstored, a large amount of umbilical cord mesenchymal stem cells can be obtained in a short time by utilizing the reagents in the kit, and the kit is convenient to use, safe and reliable.

Description

technical field [0001] The invention belongs to the technical field of stem cells, and relates to a kit for preparing umbilical cord mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells originate from the mesoderm and ectoderm in the early stages of development, and were initially found in the bone marrow. They have attracted increasing attention because of their multi-lineage differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication. , under specific induction conditions in vivo or in vitro, it can differentiate into various tissue cells such as fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, myocardium, endothelium, etc. It has a very broad clinical application prospect. [0003] Mesenchymal stem cells are mainly derived from bone marrow, umbilical cord, fat, etc. Because the number and proliferation ability of stem cells decrease significantly with age, the numbe...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A01N1/02
CPCA01N1/0221C12N5/0665C12N2500/32C12N2500/38C12N2500/90C12N2501/33C12N2501/392
Inventor 张炳强
Owner QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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