Kit for preparing umbilical cord mesenchymal stem cells

A quality stem cell and kit technology, applied in the field of stem cells, can solve the problems of donor pain, bleeding, chronic pain, infection, etc., and achieve the effects of convenient use, no serum, and low cost.

Inactive Publication Date: 2019-03-05
QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the number and proliferation ability of stem cells decrease significantly with age, the number and activity of mesenchymal stem cells derived from bone marrow and fat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example 1: Preparation and application of a kit for preparing umbilical cord mesenchymal stem cells

[0020] 1. Preparation of kit for preparing umbilical cord mesenchymal stem cells

[0021] 1. Solution configuration

[0022] The kit for preparing umbilical cord mesenchymal stem cells of the present invention includes the following reagents:

[0023] (1) Umbilical cord preservation solution preparation method: penicillin (brand Amresco, article number 0242) 200mg, streptomycin (brand Amresco, article number 0382) 200mg, amphotericin (brand Amresco, article number E437) 5mg, progesterone (Sigma, V900699- 5g) 2μg, insulin (Sigma, I0908) 10mg, vitamin C (Sigma, A5960-10mg) 2mmol, dissolved in 1000ml DMEM medium 0.22um filtration sterilization can be.

[0024] (2) Umbilical cord washing solution preparation method: Penicillin 200mg, streptomycin 200mg, amphotericin 5mg, progesterone 2μg, insulin 10mg, vitamin C 2mmol, dissolve in 1000ml normal saline 0.22um filter sterilization.

[...

Example Embodiment

[0047] Example 2: Preparation and application of a kit for preparing umbilical cord mesenchymal stem cells

[0048] 1. Preparation of kit for preparing umbilical cord mesenchymal stem cells

[0049] 1. Solution configuration

[0050] The kit for preparing umbilical cord mesenchymal stem cells of the present invention includes the following reagents:

[0051] (1) Configuration method of umbilical cord preservation solution: Penicillin 100mg, Streptomycin 100mg, Amphotericin 2.5mg, Progesterone 3μg, Insulin 30mg, Vitamin C 4mmol, dissolve in 1000ml DMEM / F12 medium 0.22um filter sterilization and then pack it can.

[0052] (2) Umbilical cord washing solution preparation method: Penicillin 100mg, streptomycin 100mg, amphotericin 2.5mg, progesterone 3μg, insulin 30mg, vitamin C 4mmol, dissolve it in 1000ml of normal saline 0.22um, filter sterilization and sub-pack.

[0053] (3) Preparation method of umbilical cord decomposition solution: collagenase II 2 g, deoxyribonuclease I 0.2 g, progest...

Example Embodiment

[0063] Example 3 Preparation and application of a kit for preparing umbilical cord mesenchymal stem cells

[0064] 1. Preparation of kit for preparing umbilical cord mesenchymal stem cells

[0065] 1. Solution configuration

[0066] The kit for preparing umbilical cord mesenchymal stem cells of the present invention includes the following reagents:

[0067] (1) Configuration method of umbilical cord preservation solution: Penicillin 150mg, Streptomycin 150mg, Amphotericin 2.5mg, Progesterone 2.5μg, Insulin 25mg, Vitamin C 3mmol, dissolve in 1000ml DMEM medium 0.22um filter sterilization and sub-pack. .

[0068] (2) Umbilical cord washing solution preparation method: penicillin 150mg, streptomycin 150mg, amphotericin 2.5mg, progesterone 2.5μg, insulin 25mg, vitamin C 3mmol, dissolve it in 1000ml normal saline 0.22um and filter sterilization.

[0069] (3) Configuration method of umbilical cord decomposition solution: collagenase II 2 g, deoxyribonuclease I 0.2 g, progesterone 2.5 μg, insu...

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PUM

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Abstract

The invention discloses a kit for preparing umbilical cord mesenchymal stem cells, and the kit is characterized in that the kit comprises an umbilical cord storage solution, an umbilical cord washingsolution, an umbilical cord decomposition solution, a cell washing solution, a cell culture solution, a cell digestion solution and a cell cryopreservation solution which are respectively bottled andstored, a large amount of umbilical cord mesenchymal stem cells can be obtained in a short time by utilizing the reagents in the kit, and the kit is convenient to use, safe and reliable.

Description

technical field [0001] The invention belongs to the technical field of stem cells, and relates to a kit for preparing umbilical cord mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells originate from the mesoderm and ectoderm in the early stages of development, and were initially found in the bone marrow. They have attracted increasing attention because of their multi-lineage differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication. , under specific induction conditions in vivo or in vitro, it can differentiate into various tissue cells such as fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, myocardium, endothelium, etc. It has a very broad clinical application prospect. [0003] Mesenchymal stem cells are mainly derived from bone marrow, umbilical cord, fat, etc. Because the number and proliferation ability of stem cells decrease significantly with age, the numbe...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A01N1/02
CPCA01N1/0221C12N5/0665C12N2500/32C12N2500/38C12N2500/90C12N2501/33C12N2501/392
Inventor 张炳强
Owner QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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