Method for preparing fermented sesame protein by using probiotics and use thereof
A technology of probiotics and sesame, applied in the field of protein processing, can solve problems such as reducing the allergy of sesame, and achieve the effects of inhibiting pathogenic microorganisms, improving allergic constitution, and being easy to absorb
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0023] Take black sesame seeds, sieve and remove impurities, add ultrapure water 12 times the weight of black sesame seeds, extract 2 times by the tissue crushing method, each extraction is crushed for 30 seconds, with an interval of 2 seconds, and then crushed for 60 seconds, with an interval of 2 seconds. Second, pulverized for 30 seconds, the extract was filtered, the filtrate was combined twice, and the pH was adjusted to 6.8 with sodium bicarbonate with a mass concentration of 5% to obtain a sesame protein solution; take Lactobacillus reuteri, add sterile physiological saline, and adjust to Concentration is 1×10 8 The bacterial liquid of CFU / mL was inoculated into the sesame protein solution, the inoculum amount was 10 mL of the sesame protein solution, and 1 mL of bacterial liquid was used. Under the conditions of 37 ° C and 70 r / min, the fermented sesame protein was shaken for 8 h, and the obtained liquid fermented sesame protein was fermented for 8 h. The solution was ...
Embodiment 2
[0025] Take white sesame seeds, sieve and remove impurities, add ultrapure water 10 times the weight of white sesame seeds, and extract twice by tissue crushing method. Second, pulverized for 30 seconds, the extract was filtered, the filtrate was combined twice, and the pH was adjusted to 7 with a mass concentration of 5% sodium bicarbonate to obtain a sesame protein solution; take Lactobacillus reuteri, add sterile physiological saline, and adjust to the concentration 1×10 10 The bacterial liquid of CFU / mL was inoculated into the sesame protein solution, the inoculum amount was 15 mL of the sesame protein solution, and 1 mL of bacterial liquid was used. The solution was concentrated to 45° C. to measure the relative density of 1.05, and then dried under reduced pressure to powder to obtain powdered fermented sesame protein.
Embodiment 3
[0027] Take yellow sesame seeds, sieve and remove impurities, add ultrapure water 6 times the weight of yellow sesame seeds, and extract twice by tissue crushing method. Second, pulverized for 30 seconds, the extract was filtered, the filtrate was combined twice, and the pH was adjusted to 6.7 with a mass concentration of 5% sodium bicarbonate to obtain a sesame protein solution; Lactobacillus reuteri was taken, and sterile saline was added to adjust to the concentration 3×10 8 The bacterial liquid of CFU / mL was inoculated into the sesame protein solution, the inoculum amount was 13 mL of the sesame protein solution, and 1 mL of bacterial liquid was used. Under the conditions of 40 ° C and 50 r / min, the fermented sesame protein was shaken for 6 h, and the obtained liquid fermented sesame protein was The solution was concentrated to 45° C. to measure the relative density of 1.1, and spray-dried to obtain powdered fermented sesame protein.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More