Biological hybrid gradient hydrogel scaffold as well as preparation method and application thereof

Abstract

The invention discloses a biological hybrid gradient hydrogel scaffold as well as a preparation method and application thereof. The hydrogel is prepared from acryloyl glycine and N-acroloyl (trihydroxymethyl) aminomethane as raw materials. The preparation method of the scaffold comprises the following steps: melting the gel, mixing tricalcium phosphate, performing 3D printing to obtain a scaffoldof which the bottom is doped with tricalcium phosphate and an upper hydrogel scaffold, and printing a growth factor on the hydrogel scaffold by utilizing a piezoelectric technology. According to the doped tricalcium phosphate, the bone guide ability of the scaffold is increased, and the mechanical strength of the scaffold is improved. The upper layer of the hydrogel is capable of improving the lubricating property of a cartilage substitute material, and due to the existence of the factor, cartilage repair is promoted. The gradient scaffold disclosed by the invention is integrally molded and issimple in formula, safe in component and mild in preparation conditions, and the problem of interface bonding between the cartilage and subchondral bone is solved. Meanwhile, the binding capacity ofgel-like cartilage repair materials and host bones is improved, and due to the supported tricalcium phosphate and the growth factor, the integrated repair ability of the cartilage and the subchondralbone is improved.

Description

technical field [0001] The invention belongs to the field of hydrogel technology, and relates to a method for preparing a biohybrid gradient hydrogel scaffold for integrated repair of cartilage and subchondral bone, more specifically, to a method using acryloyl glycinamide and N- A method for preparing acryloyl (trihydroxymethyl) aminomethane copolymerized supramolecular hydrogel as a monomer, and a method for printing this hydrogel into a gradient scaffold by using an integrated printing technology. The forming method is simple, and the scaffold simultaneously loads β -TCP and TGF-β1, improve the ability of cartilage and subchondral bone integration repair. Background technique [0002] The prevalence of articular cartilage damage is relatively high. According to reports, about 60% of patients undergoing arthroscopic examination have articular cartilage damage in varying degrees, and the prevalence rate of people over 65 years old is as high as 60%-90%. However, since arti...

AI Technical Summary

Problems solved by technology

However, the growth factor itself has problems such as short half-life, easy inactivation, high price and risk of side effects, and the subsequent sterilization and storage of growth factor-loaded scaffolds are also difficult

Growth factors usually can only be loaded on the surface of the scaffold in a specific way after the scaffold is formed, and the process is complex and cumbersome

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