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Efficient porous superparamagnetic fluoride ion dual fluorescence detection probe and preparation method thereof

A technology for detecting probes and superparamagnetism, applied in fluorescence/phosphorescence, chemical instruments and methods, measuring devices, etc. Toxicity and other issues, to achieve excellent superparamagnetic properties, good cell permeability, and low cytotoxicity

Active Publication Date: 2021-12-24
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the reported probes have many shortcomings such as potential biological toxicity, low specific surface area, unsatisfactory dispersibility, and difficulty in practical application with the naked eye, and most of them are single fluorophores, and the probe fluorescence is weak. The selectivity and sensitivity of metal ions are not high, and its application in the ecological environment is questionable, which affects the further application in real life

Method used

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  • Efficient porous superparamagnetic fluoride ion dual fluorescence detection probe and preparation method thereof
  • Efficient porous superparamagnetic fluoride ion dual fluorescence detection probe and preparation method thereof
  • Efficient porous superparamagnetic fluoride ion dual fluorescence detection probe and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0104] Embodiment 1 hydrophilic Fe 3 o 4 Synthesis of cores, organosilane-functionalized carbon dots (SiCDs) in dilute solutions and autofluorescent markers (Fe 3 o 4 @mSiO 2 -SiCDs) preparation

[0105] 1. Hydrophilic Fe 3 o 4 The synthesis of the core includes the following steps:

[0106] (1) 0.325g anhydrous FeCl 3 and 0.4g of trisodium citrate were dissolved in 40mL of ethylene glycol, and magnetically stirred until an orange-yellow solution was formed;

[0107] (2) Under magnetic stirring, add 3 g of sodium acetate until a uniform yellow-brown solution is obtained, transfer the solution to a 50 mL Teflon-lined autoclave, and keep it at 200 ° C for 10 h; cool to room temperature Finally, the black product was separated by magnetic force, washed 5 times with ethanol, washed 1 time in deionized water, and dried in vacuum at 40 °C to obtain hydrophilic Fe 3 o 4 core.

[0108] 2. The synthesis of organosilane functionalized carbon dots (SiCDs) dilute solution, comp...

Embodiment 2

[0117] Example 2 DTPA anhydride and magnetic nanoparticles coated with fluorescent silica (Fe 3 o 4 @mSiO 2 -SiCDs@DTPA-Ni 2+ , FSMN) preparation

[0118] 1, the preparation process of diethylenetriaminepentaacetic acid (DTPA) anhydride is as follows figure 1 As shown, it specifically includes the following steps:

[0119] (1) 33.7g of diethylenetriaminepentaacetic acid (DTPA) was added to a 250mL round bottom flask containing 40mL of pyridine, and then 33mL of acetic anhydride was added;

[0120] (2) Pass N 2 Dissolved oxygen was removed, and the solution was vigorously stirred (1000 rpm) at 70°C for 24 hours to obtain DTPA anhydride;

[0121] (3) Wash DTPA anhydride twice in excess acetic anhydride, then wash three times in excess ether, and dry in vacuum to obtain diethylenetriaminepentaacetic dianhydride.

[0122] 2. Fluorescent silica-coated magnetic nanoparticles (Fe 3 o 4 @mSiO 2 -SiCDs@DTPA-Ni 2+ , FSMN) preparation method, comprises the following steps:

...

Embodiment 3

[0126] Example 3 Synthesis of pure carbon dots (NCDs) and fluoride ion dual fluorescent detection probes (Fe 3 o 4 @mSiO 2 -SiCDs@DTPA-Ni 2+ -NCDs, FSMN-NCDs) preparation

[0127] 1. The preparation process of pure carbon dots (NCDs) is as follows: figure 2 As shown, it specifically includes the following steps:

[0128] (1) Mix 20 mL of deionized water with 0.84 g of trisodium citrate, transfer it to a 50 mL polytetrafluoroethylene-lined hydrothermal reactor, and react at a constant temperature of 200 ° C for 5 h;

[0129] (2) After cooling, add ammonia solution and react at 200°C for another 10 hours, dialyze the obtained yellow-brown solution in a dialysis bag, soak it in 1000mL deionized water, and stir it with gentle magnetic force (200~600rpm) at the same time overnight;

[0130] (3) After adjusting the pH value to 6, the NCDs solution was obtained.

[0131] 2. Fluoride ion dual fluorescent detection probe (Fe 3 o 4 @mSiO 2 -SiCDs@DTPA-Ni 2+ -NCDs, the prepar...

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Abstract

The invention discloses a high-efficiency porous superparamagnetic fluoride ion double fluorescence detection probe and a preparation method thereof. The probes include self-fluorescent markers and quenched fluorescent probes; the self-fluorescent markers are mesoporous silica containing organosilane functionalized carbon points, and the quenched fluorescent probes are pure carbon points and nickel Chelates of ions. The probe has dual fluorophores and is reusable. It can monitor the real-time concentration of fluoride ions in the water environment very well. The minimum detection limit of F ions is as low as 65 nM, and the linear response range is 1-25 μM, which has good sensitivity. and selectivity; at the same time, it can efficiently adsorb and remove fluoride ions, and the removal efficiency of fluoride ions in tap water is as high as 96%, and can be recycled repeatedly; and has excellent biocompatibility and good cell permeability And low cytotoxicity, it shows great potential in the direction of detecting fluoride ions in the internal and external environment of cells, it is green and environmentally friendly, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of functional nanometer materials. More specifically, it relates to a high-efficiency porous superparamagnetic fluoride ion dual fluorescence detection probe and a preparation method thereof. Background technique [0002] Fluoride ion (F - ) are widely distributed in environmental systems and biological processes. Adequate intake of fluoride ions is beneficial to the health of teeth and bones, but too much or too little intake of fluoride ions will have adverse effects on the human body and may cause systemic diseases, such as Fluorosis and osteoporosis etc. Therefore, it is extremely important for human beings to accurately detect and control the concentration of fluoride ions in water resources. [0003] Various efforts have been attempted by the scientific community to develop new methods and strategies for the detection of fluoride ions, including photophysical mechanisms and fluoride-induced chemical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C02F1/28
CPCG01N21/6408G01N21/6428C02F1/28C02F2101/14G01N2021/6432
Inventor 庄琳李晓磊许跃刘伍明王玮郑昂
Owner SUN YAT SEN UNIV
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