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Measuring device and measuring method for primary productivity of phytoplankton based on chlorophyll fluorescence

A technology of chlorophyll fluorescence and primary productivity, which is applied in the field of resources and the environment, can solve the lack of devices for measuring the primary vitality of phytoplankton in actual water bodies, measure the fluorescence kinetic curve of phytoplankton, and the fluorescence kinetic measurement method of primary productivity stays in the laboratory Sample analysis stage and other issues, to achieve the effect of compact structure

Pending Publication Date: 2019-03-19
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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Problems solved by technology

However, this technical method and technology cannot measure the fluorescence kinetic curve of phytoplankton under natural environment light conditions, and obtain the core fluorescence kinetic parameters used to calculate the primary productivity, resulting in the primary productivity fluorescence kinetic measurement method still staying at the stage of laboratory sample analysis , so far there is still a lack of devices that can be used to measure the primary vitality of phytoplankton in actual water bodies

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  • Measuring device and measuring method for primary productivity of phytoplankton based on chlorophyll fluorescence
  • Measuring device and measuring method for primary productivity of phytoplankton based on chlorophyll fluorescence
  • Measuring device and measuring method for primary productivity of phytoplankton based on chlorophyll fluorescence

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Embodiment Construction

[0026] Such as figure 1 As shown, a method for measuring the primary productivity of phytoplankton based on chlorophyll fluorescence, the specific steps are as follows: (1) Measure the spectral intensity of the characteristic absorption band of phytoplankton in the actual natural light, and use the controllable light source of the corresponding band to simulate the natural environment illumination. Under the condition, first measure the fluorescence kinetic curve of single turnover ST, and analyze and obtain the fluorescence kinetic parameter under the dark-adapted condition, that is, the background fluorescence F 0 and the functional absorption cross section σ 0 ;

[0027] (2) Then irradiate the phytoplankton samples with the simulated light source for more than 60s, and use the dependence of phytoplankton on the light history to measure the multi-turnover MT and relaxation RE fluorescence kinetic curves in the 50ms off-off interval of the simulated light to obtain the fluor...

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Abstract

The invention discloses a measuring device and a measuring method for primary productivity of phytoplankton based on chlorophyll fluorescence. The measuring method comprises the following steps: in ashielding condition, irradiating an alga sample continuously for over 60 s through a simulating light source; measuring single period and relaxation fluorescence dynamic curves in an illuminating condition when the gap is closed transiently after simulated irradiation for 50 ms to obtain fluorescent dynamic parameters; measuring an alga chlorophyll fluorescence dynamic curve by taking a laser diode array as an inductive laser light source and a photomultiplier as a fluorescent detector; and designing an underwater photosynthetic effective radiation measuring unit by using a crown-shaped optical collector and a multi-band optical detector array to measure the natural environmental spectra corresponding to the alga absorption characteristic. The method is compact in structure, small in sizeand low in power consumption, can measure the primary productivity of phytoplankton in situ quickly and provides a novel means for monitoring and prewarning red tide and water bloom disasters and investigating and evaluating primary productivity of sea.

Description

technical field [0001] The invention relates to the field of resources and environment, and the field of marine technology, in particular to a measuring device and method for primary productivity of phytoplankton based on chlorophyll fluorescence. Background technique [0002] Phytoplankton is a general term for tiny plants that live in a planktonic way in water bodies. Although their biomass is less than 1% of the total global plant biomass, their primary productivity accounts for more than 40% of the global total primary productivity. It plays a key role in maintaining the normal operation of the global ecosystem. The relationship between the primary production of phytoplankton and the growth of phytoplankton itself, the relationship with secondary production and the microfood cycle, and its position in the global carbon cycle are the core topics of major global research programs such as GLOBEC, LOICS, and JGOFS. . The rapid and accurate acquisition of ocean primary prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/01G01N21/6402G01N21/6486
Inventor 殷高方赵南京马明俊王翔陈敏甘婷婷孟德硕杨瑞芳方丽董鸣刘建国刘文清
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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