Measuring device and measuring method for primary productivity of phytoplankton based on chlorophyll fluorescence
A technology of chlorophyll fluorescence and primary productivity, which is applied in the field of resources and the environment, can solve the lack of devices for measuring the primary vitality of phytoplankton in actual water bodies, measure the fluorescence kinetic curve of phytoplankton, and the fluorescence kinetic measurement method of primary productivity stays in the laboratory Sample analysis stage and other issues, to achieve the effect of compact structure
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[0026] Such as figure 1 As shown, a method for measuring the primary productivity of phytoplankton based on chlorophyll fluorescence, the specific steps are as follows: (1) Measure the spectral intensity of the characteristic absorption band of phytoplankton in the actual natural light, and use the controllable light source of the corresponding band to simulate the natural environment illumination. Under the condition, first measure the fluorescence kinetic curve of single turnover ST, and analyze and obtain the fluorescence kinetic parameter under the dark-adapted condition, that is, the background fluorescence F 0 and the functional absorption cross section σ 0 ;
[0027] (2) Then irradiate the phytoplankton samples with the simulated light source for more than 60s, and use the dependence of phytoplankton on the light history to measure the multi-turnover MT and relaxation RE fluorescence kinetic curves in the 50ms off-off interval of the simulated light to obtain the fluor...
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