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Tissue culture technique of solanum lyratum

A tissue culture and technology technology, applied in the field of plant tissue culture, can solve problems such as protection and separation of unfavorable high-quality germplasm resources, restricting the promotion and cultivation of high-quality Baiying, and achieve the effect of accelerating popularization and resource development and utilization.

Inactive Publication Date: 2019-03-22
韦宇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Bai Ying is mainly propagated by seeds, but the separation of offspring traits due to seed propagation is not conducive to the protection of high-quality germplasm resources, and restricts the popularization and planting of high-quality Bai Ying.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Induction of clustered buds: Select the budding stems of young trees that grow vigorously and have no diseases and insect pests in the current year as explants, soak them in an aqueous solution of detergent for 11 minutes, then rinse them with tap water for 16 minutes, dry the surface moisture and set aside. Soak in 75% ethanol for 11s in ultra-clean workbench, sterilize with 0.1% mercuric chloride solution for 14min, wash with sterile water for 9 times, dry and cut into 6.2cm long stems and inoculate them on the induction medium. Cultivate in total darkness at 26°C for 21 days, then place in light for 16 hours a day, light intensity 1600x, and culture temperature 26°C for 44 days to induce the formation of clustered buds, with an induction rate of 69%. The induction medium is MS+8mg / L6-BA+6.5mg / L NAA+6.5mmol / L La(NO 3 ) 2 +6.0% sucrose+0.80% agar+0.11% activated carbon, the pH value is 5.6.

[0013] (2) Proliferation culture: Transfer the clustered buds obtained ...

Embodiment 2

[0017] (1) Induction of clustered buds: select the budding stems of young trees that grow vigorously and have no diseases and insect pests in the current year as explants, soak them in aqueous solution of detergent for 13 minutes, then rinse them with tap water for 20 minutes, dry the surface moisture and set aside. Soak in 75% ethanol for 16s in an ultra-clean workbench, sterilize with 0.1% mercuric chloride solution for 17min, wash with sterile water for 7 times, dry and cut into 1.8cm-long stems and inoculate them on the induction medium. Cultivate in total darkness at 27°C for 23 days, then place in the light for 18 hours a day, the light intensity is 1600x, and the culture temperature is 27°C for 32 days to induce the formation of clustered buds, and the induction rate is 79.9%. The induction medium is MS+9mg / L6-BA+1.2mg / L NAA+0.6mmol / L La(NO 3 ) 2 +4.0% sucrose+0.4% agar+0.5% activated carbon, the pH value is 5.6.

[0018] (2) Proliferation culture: Transfer the cluste...

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PUM

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Abstract

The invention discloses a tissue culture technique of solanum lyratum, and relates to a breeding method for obtaining high-quality seedlings by carrying out in vitro rapid propagation on the solanum lyratum. The technique uses stems with buds as explants, and comprises explant disinfection, cluster bud induction, rooting culture, acclimatization and transplanting, and the like, so that a solanum lyratum tissue culture rapid propagation system is established; the tissue culture technique of the solanum lyratum has an important practical significance for popularization of an improved variety ofthe solanum lyratum as well as resource exploitation and utilization.

Description

technical field [0001] The invention relates to a method for plant tissue culture in plant biotechnology, in particular to a technique for tissue culture of Bai Ying. Background technique [0002] Baiying aliases: white hair rattan, Shu Yangquan, hair gourd. Distributed in most parts of the country. Perennial herb, collected whole in summer and autumn, washed, cut into sections, and sun-dried. The smell is sweet, pungent, bitter, slightly cold in nature. It has the functions of clearing away heat and detoxification, expelling wind and dampness. For a long time, the supply has been in short supply, and the wild resources of Baiying have been depleted due to man-made plundering and deforestation. Therefore, it is necessary to protect its resources. In recent years, the development of Baiying resources has reached a certain scale, and GAP planting bases have been established in the north and south provinces of my country, which has played a certain role in promoting the pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 韦宇
Owner 韦宇