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Method for rapidly propagating Stewartia sinensis by using stem tips

A purple stem and shoot tip technology, applied in the field of tissue culture, can solve the problems of low survival rate of seedlings, high deformity rate of purple stem tissue culture seedlings, hazards to operators and the environment, etc., achieve enhanced disinfection effect, reduce purple stem tip Damage and deformity reduction effect

Inactive Publication Date: 2019-03-22
吕诗林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to protect this endangered species, many researchers have used tissue culture to obtain tissue culture seedlings of Adenophora adenophora, and have achieved certain results. However, the existing Adenophora tissue culture generally uses the The traditional alcohol mercuric liter disinfection method, because mercuric liter is highly toxic, is harmful to the operator and the environment, and the deformity rate of the obtained purple stem tissue culture seedlings is high, and the survival rate of the seedlings is low, so further improvement is needed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method of rapidly propagating purple stems using shoot tips, comprising:

[0020] The explant preparation of the purple stem is specifically: cutting the young and tender stem tip of the purple stem, soaking the stem tip with a 2% aqueous solution of detergent for 30 minutes, taking it out, and washing it with running water for 10-15 minutes, Then put it into an alcohol solution with a mass fraction of 70%, treat it at a current of 0.5mA for 30s, take it out, and rinse it with sterile water for 2-3 times to obtain the final product.

[0021] Adventitious bud induction culture, specifically, the explants were cut into 2-3mm segments and transferred to adventitious bud induction medium, cultured at 22-26°C for 2 days in the dark, and then at 25-28 ℃, light intensity 2500-2800lux, and light time 12h / d, cultured for 20 days to obtain adventitious buds, wherein, the medium for inducing adventitious buds is: MS+1.5mg / L 6-BA+1.0mg / L GA3+ 1.0mg / L IBA+0.5mg / L 2,4-D.

[0022]...

Embodiment 2

[0026] A method of rapidly propagating purple stems using shoot tips, comprising:

[0027] The explant preparation of the purple stem is specifically: cutting the young and tender stem tip of the purple stem, soaking the stem tip with a 2% aqueous solution of detergent for 30 minutes, taking it out, and washing it with running water for 10-15 minutes, Then put it into an alcohol solution with a mass fraction of 70%, treat it at a current of 1mA for 25s, take it out, and rinse it with sterile water for 2-3 times to obtain the final product.

[0028] Adventitious bud induction culture, specifically, the explants were cut into 2-3mm segments and transferred to adventitious bud induction medium, cultured at 22-26°C for 2 days in the dark, and then at 25-28 ℃, light intensity 2500-2800lux, and light time 12h / d, cultured for 20 days to obtain adventitious buds, wherein, the medium for inducing adventitious buds is: MS+1.8mg / L 6-BA+1.2mg / L GA3+ 1.2mg / L IBA+0.8mg / L 2,4-D.

[0029] C...

Embodiment 3

[0033] A method of rapidly propagating purple stems using shoot tips, comprising:

[0034] The explant preparation of the purple stem is specifically: cutting the young and tender stem tip of the purple stem, soaking the stem tip with a 2% aqueous solution of detergent for 30 minutes, taking it out, and washing it with running water for 10-15 minutes, Then put it into an alcohol solution with a mass fraction of 70%, treat it at a current of 2mA for 20s, take it out, and rinse it with sterile water for 2-3 times to obtain the final product.

[0035] Adventitious bud induction culture, specifically, the explants were cut into 2-3mm segments and transferred to adventitious bud induction medium, cultured at 22-26°C for 2 days in the dark, and then at 25-28 ℃, light intensity 2500-2800lux, and light time 12h / d, cultured for 20 days to obtain adventitious buds, wherein, the medium for inducing adventitious buds is: MS+2.0mg / L 6-BA+1.5mg / L GA3+ 1.5mg / L IBA+1.0mg / L 2,4-D.

[0036] C...

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Abstract

The invention discloses a method for rapidly propagating Stewartia sinensis by using stem tips. The method comprises the step of preparing Stewartia sinensis explants, wherein the method comprises thespecific steps of scissoring tender stem tips of the Stewartia sinensis, soaking the stem tips with a 2% (mass percent) aqueous solution of a detergent for 30min, taking out the stem tips, flushing the stem tips for 10 to 15 minutes with flowing water, then, placing the stem tips into a 70% (mass percent) alcoholic solution, carrying out treatment for 20 to 30 seconds under the current of 0.5mA to 2mA, taking out the stem tips, and flushing the stem tips with sterile water for 2 to 3 times, thereby obtaining the Stewartia sinensis explants. According to the method, the stem tips of the Stewartia sinensis are disinfected in an alcohol and microcurrent matched manner, and thus, the method is friendly to operating personnel and environments; the deformed seedling rate of obtained Stewartia sinensis tissue culture seedlings is as low as 0.9%, and the survival rate of young seedlings reaches up to 97.5%.

Description

technical field [0001] The present invention relates to the field of tissue culture. More specifically, the present invention relates to a method for rapid propagation of purple stems using shoot tips. Background technique [0002] Stewartia sinensis (Latin name: Stewartia sinensis Rehd.et Wils.), a perennial woody plant of the genus Stewartia sinensis in the family Theaceae, is a unique relic plant in China. In order to protect this endangered species, many researchers have used tissue culture to obtain tissue culture seedlings of Adenophora adenophora, and have achieved certain results. However, the existing Adenophora tissue culture generally uses the The traditional alcohol mercuric liter disinfection method, because mercuric liter is highly toxic, is harmful to the operator and the environment, and the obtained Adenophora adenophora tissue culture seedlings have a high deformity rate and a low survival rate of the seedlings, so further improvement is needed. Contents...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/00A01H4/008
Inventor 吕诗林
Owner 吕诗林
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