Method for preparing lutein visual function intensifying egg by nondestructively penetrating avian eggshell membrane
A technology of lutein and eggshell membrane, which is applied in the field of poultry egg processing, can solve the problems of lack of cultural connotation, high production cost, and long production cycle, and achieve the effects of improving penetration efficiency, reducing economic costs, and improving vision
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Embodiment 1
[0035] 1) Pretreatment of eggs: Clean the surface of the eggshell and dry it, then soak it in 1% acetic acid solution for 30 minutes, rinse it again and dry it for later use;
[0036] 2) Enzymatic hydrolysis treatment of eggshell membrane: immerse pretreated eggs in a combined enzymatic hydrolysis reagent solution formed by alkaline protease with a concentration of 0.8g / L and L-cysteine with a concentration of 10g / L ,
[0037] 3) Lutein penetrant treatment and vacuum packaging: mix the eggs treated in step 2) with lutein penetrant at a weight ratio of 1:2, and then vacuum pack;
[0038] 4) Ultra-high pressure treatment: place the packaged eggs in an ultra-high pressure device for ultra-high pressure treatment to obtain fortified eggs, where the pressure is 100MPa and the time is 120min.
[0039] Wherein, the lutein penetrant in this embodiment is obtained by dissolving 1% water-soluble lutein in distilled water, adding 1% vitamin C, and stirring in a water bath at 50°C unti...
Embodiment 2
[0043] 1) Pretreatment of eggs: Clean the surface of the eggshell and dry it, then soak it in 2% acetic acid solution for 20 minutes, rinse it again and dry it for later use;
[0044] 2) Enzymatic hydrolysis treatment of eggshell membrane: immerse the pretreated egg in the combined enzymatic hydrolysis reagent solution;
[0045] 3) Lutein penetrant treatment and vacuum packaging: mix the eggs treated in step 2) with lutein penetrant at a weight ratio of 1:2, and then vacuum pack;
[0046] 4) Ultra-high pressure treatment: place the packaged eggs in an ultra-high pressure device for ultra-high pressure treatment to obtain fortified eggs, where the pressure is 200MPa and the time is 100min.
[0047] In this embodiment, the combined enzymolysis reagent solution is prepared from alkaline protease with a concentration of 1 g / L and vitamin C with a concentration of 15 g / L; The reaction was carried out under the conditions of ℃ and pH 8.5 for 16 hours.
[0048] In this embodiment, ...
Embodiment 3
[0050] 1) Pretreatment of eggs:
[0051] Clean the surface of the eggshell and dry it, then soak it in 4% citric acid solution for 20 minutes, rinse it again and dry it for later use;
[0052] 2) Enzymatic hydrolysis treatment of eggshell membrane: immerse the pretreated egg in the combined enzymatic hydrolysis reagent solution;
[0053] 3), lutein penetrant treatment, vacuum packaging: mix the eggs treated in step 2) with lutein penetrant at a weight ratio of 1:3, and then vacuum pack;
[0054] 4) Ultra-high pressure treatment: place the packaged eggs in an ultra-high pressure device for ultra-high pressure treatment to obtain fortified eggs. The pressure is 500 MPa and the time is 10 minutes.
[0055] In an embodiment, the combined enzymolysis reagent solution is prepared by papain with a concentration of 0.5g / L and sodium sulfite with a concentration of 20g / L. The reaction was carried out for 12 hours under the condition of pH 6.5.
[0056] In this embodiment, the lutein...
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