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Functions and uses of e2f6 inhibitors

A technology of E2F6, 1.E2F6, applied in the field of life sciences, can solve problems such as the termination of cancer cell induced drug resistance

Active Publication Date: 2020-10-30
北京金岱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although oral temozolomide increases overall survival in glioblastoma patients, cancer cell-induced resistance terminates further therapy
Therefore, overcoming the drug resistance of glioma cells and completely treating glioblastoma is still a major challenge in current medicine.

Method used

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  • Functions and uses of e2f6 inhibitors
  • Functions and uses of e2f6 inhibitors
  • Functions and uses of e2f6 inhibitors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] 1.1 Cell culture

[0109] Human glioma cell U87 was purchased from ATCC (ATCC Company, USA), and N5, N9 and N33 primary cells were cultured from glioma tissue excised from glioma patients. The surgically excised gliomas were quickly stored in serum-free medium and kept in an ice bath. Cut the tissue into 0.5mm in the sterile operating table 3 The small pieces are grown in serum-containing medium, and cultured as tumor cells after the tumor cells fall off and adhere to the wall. The cells were cultured in DMEM medium containing 10% heat-inactivated calf serum (FBS, Hyclone, USA). Each EGFRvIII cell line was obtained from lentiviral transfection constructed with GV341 plasmid expressing EGFRvIII cDNA, followed by puromycin selection for 7 days. All these cells were grown at a constant temperature of 37 °C, 5% CO 2 in a humid incubator. E2F6 overexpression and E2F6 knockdown lentiviruses were purchased from GeneKy.

[0110] The GeCKO plasmid library is a genome-wide ...

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Abstract

Disclosed is a use of E2F6 as a target capable of increasing the sensitivity of temozolomide. Temozolomide is used for treating glioblastoma. E2F6 inhibitor and temozolomide treatment can increase the curability of glioblastoma. The invention provides scientific evidence for the pathogenesis and clinical treatment of glioblastoma from the clinical patient sample level, cell function level and molecular level.

Description

technical field [0001] The invention belongs to the technical field of life sciences, and specifically relates to the function and application of E2F6 inhibitors. Background technique [0002] Glioblastoma is the most common primary intracranial malignant tumor, with strong proliferation and invasion ability, and easy recurrence after operation. Glioblastoma grows rapidly, and 70% to 80% of patients have a disease course of 3 to 6 months, and only 10% of patients have a disease course of more than 1 year. Those with a longer course of disease may evolve from low-grade astrocytomas. Due to the rapid growth of the tumor, extensive cerebral edema, and obvious symptoms of increased intracranial pressure, all patients had symptoms of headache and vomiting. Optic disc edema has headache, mental changes, limb weakness, vomiting, disturbance of consciousness and speech disturbance. The tumor infiltrates and destroys brain tissue, causing a series of focal symptoms. Patients have ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7088A61K31/4188A61P35/00
CPCA61K31/4188A61K31/7088A61P35/00A61K2300/00
Inventor 康春生江涛
Owner 北京金岱生物科技有限公司
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