Method for efficiently synthesizing sanguinarine and chelerythrine through mechanical damage

Abstract

The invention provides a method for efficiently synthesizing sanguinarine and chelerythrine through mechanical damage. The method specifically comprises the following steps: selecting sterile tissue culture seedlings after 16 hours of light culture and 8 hours of dark culture, a lighting intensity of 4500-9000 Lx and 60 days of culture; pricking 8-12 holes in seedling leaves of the sterile tissueculture seedlings by using a sterile needle, and culturing for 24-120 hours in an MS (Murashige&Skoog) culture medium at 25 DEG C in a light cycle of 16 hours; extracting metabolite from the sterile tissue culture seedlings after hole pricking treatment culture in the step (2), and carrying out quantitative analysis, thereby obtaining sanguinarine and chelerythrine. According to the method, through mechanical damage of macleaya cordata sterile tissue culture seedlings, expression of genes P6H and DBOX which join in synthesis of sanguinarine and chelerythrine in macleaya cordata is promoted, furthermore efficient synthesis of the sanguinarine and chelerythrine is promoted, the metabolite is extracted from the cultured sterile tissue culture seedlings, and thus the sanguinarine and chelerythrine are efficiently prepared.

Description

technical field [0001] The invention relates to a method for efficiently synthesizing sanguinarine and chelerythrine by utilizing mechanical damage. technical background [0002] bo fall back [Macleaya cordata(Willd) R .B] is a perennial plant of the genus Papaveraceae, which is an important traditional medicinal plant, and its active ingredients are mainly isoquinoline alkaloids. Benphenanthridine alkaloids (abbreviated as BIAs) are a class of diverse alkaloids, these compounds have a wide range of biological activities, including sanguinarine, chelerythrine, protopine, allocryptine, etc. Sanguinarine has a wide range of biological activities, including strong anti-tumor, anti-bacterial and anti-inflammatory. In addition, sanguinarine and chelerythrine, as natural growth promoters, have been widely used to replace antibiotic growth promoters in animal husbandry. In 2006, following the European Union's complete ban on the addition of antibiotic growth promoters in feeding...

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Problems solved by technology

However, there is no report about the impact of mechanical damage on BIAs in the tissue culture of Boluohui at present. The present invention intends to provide a method for efficiently synthesizing sanguinarine and chelerythrine by using mechanical damage.

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