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Breeding method of jatropha curcas transgenic seedlings

A technology of transgenic seedlings and tung seeds, which is applied in grafting and other directions, can solve the problems of poor root development, no rooting, and low survival rate of transgenic seedlings, and achieve the effect of speeding up the breeding process

Active Publication Date: 2019-04-02
ZHOUKOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Jatropha jatropha mediated transformation of exogenous genes to obtain transgenic regenerated strains. The exogenous gene has been transferred into Jatropha jatropha through molecular detection, and the transformed transgenic seedlings are plants formed through tissue culture; due to the expression of exogenous genes, it is possible that Resulting in poor root development or no rooting of transgenic seedlings, resulting in low survival rate when transplanted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Jatropha seeds are sown in soil irrigated with water; when two cotyledons grow out of Jatropha seedlings, it is used as a preliminary rootstock; the Jatropha transgenic seedlings obtained by preparing the tissue culture method are used as scions;

[0032] Soak the scion in a 0.1% carbendazim solution for at least 1 hour;

[0033] Remove the top of the prepared rootstock with a knife, split the prepared rootstock with a 1cm incision from the center of the hypocotyl down to make the rootstock; cut the lower part of the soaked scion into a wedge that matches the cut of the rootstock, and then insert the wedge-shaped part into the rootstock the incision;

[0034] Drop agarose aqueous solution at a temperature of 45°C into the incision, so that the agarose gel covers the incision; the mass concentration of the agarose aqueous solution is 0.4%, and the agarose aqueous solution forms a gel when the temperature drops to 35-45°C; Cover the scion and the rootstock with plastic f...

Embodiment 2

[0038] Method is with embodiment 1, and difference is:

[0039] (1) Soak the scion in a 0.2% carbendazim solution for at least 1 hour;

[0040] (2) Splitting the 1.2cm otch of the prepared rootstock;

[0041] (3) Aqueous agarose solution with a temperature of 44° C. is dripped into the incision so that the agarose gel covers the incision; the mass concentration of the aqueous agarose solution is 0.3%; scion and stock are placed at a temperature of 30° C. and a humidity of 85 to 88% inside the greenhouse;

[0042] (4) The survival rate of Jatropha transgenic seedlings is 92%.

Embodiment 3

[0044] Method is with embodiment 1, and difference is:

[0045] (1) Soak the scion in a carbendazim solution with a mass concentration of 0.3% for at least 1 hour;

[0046] (2) Splitting the 1.5cm otch of the prepared rootstock;

[0047] (3) Aqueous agarose solution with a temperature of 43° C. is dripped into the incision so that the agarose gel covers the incision; the mass concentration of the aqueous agarose solution is 0.3%; the scion and rootstock are placed at a temperature of 35° C. 90% inside the greenhouse;

[0048] (4) The survival rate of Jatropha transgenic seedlings is 93%.

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Abstract

The invention relates to a breeding method of jatropha curcas transgenic seedlings. The breeding method of the jatropha curcas transgenic seedlings comprises the following steps: (1) when jatropha curcas seed seedlings grow two cotyledons, using the jatropha curcas seed seedlings as reserve rootstocks; using jatropha curcas transgenic seedlings as scions; (2) soaking the scions in a carbendazim solution; (3) removing the top end of the reserve rootstock, and splitting the an incision downwards from the center of the hypocotyl to prepare a rootstock; cutting the low part of the scion into a wedge shape to be inserted into the incision; (4) dropping an agar sugar solution into the incision; wrapping with a sealing film, covering with a plastic film, and placing in greenhouse for breeding; and (5) removing the plastic film after two weeks, continuing the breeding under a shading condition for two days, removing the shading, and continuing the breeding. According to the method disclosed bythe invention, the jatropha curcas transgenic seedlings are grafted onto the robust jatropha curcas seed seedlings, so that the robust jatropha curcas transgenic seedlings are bred, and the jatrophacurcas transgenic breeding process can be greatly accelerated.

Description

technical field [0001] The invention relates to the technical field of grafting and planting, in particular to a method for breeding transgenic seedlings of jatropha. Background technique [0002] Jatropha Jatropha mediated transformation of exogenous genes to obtain transgenic regenerated strains. The exogenous gene has been transferred into Jatropha Jatropha through molecular detection, and the transformed transgenic seedlings are plants formed through tissue culture. Due to the expression of exogenous genes, it is possible that This results in poor root development or no rooting of transgenic seedlings, resulting in low survival rate when transplanted. Contents of the invention [0003] The purpose of the present invention is to provide a kind of jatropha transgenic seedling breeding method, for the jatropha transgenic seedlings that are not easy to take root, the jatropha transgenic seedlings are grafted onto the robust jatropha seedlings to improve the survival rate. ...

Claims

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Application Information

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IPC IPC(8): A01G2/30
CPCA01G2/30
Inventor 李超琼徐增富田辉辉王雪芹胡利宗刘红占王俊生
Owner ZHOUKOU NORMAL UNIV
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