Breeding method of jatropha curcas transgenic seedlings
A technology of transgenic seedlings and tung seeds, which is applied in grafting and other directions, can solve the problems of poor root development, no rooting, and low survival rate of transgenic seedlings, and achieve the effect of speeding up the breeding process
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[0030] Example 1
[0031] Sow the Jatropha seeds in the soil soaked with water; when the Jatropha Jatropha seedlings grow two cotyledons, use them as a preliminary stock; prepare the Jatropha Jatropha transgenic seedlings obtained by tissue culture as a scion;
[0032] Soak the scion in a 0.1% carbendazim solution for at least 1 hour;
[0033] Use a knife to remove the top of the prepared rootstock, split the prepared rootstock from the middle of the hypocotyl down to a 1cm incision to make a rootstock; cut the lower part of the soaked scion into a wedge that matches the incision of the rootstock, and then insert the wedge-shaped part into the rootstock The incision
[0034] Drop the agarose aqueous solution at a temperature of 45°C into the incision, so that the agarose gel covers the incision; the mass concentration of the agarose aqueous solution is 0.4%, and the agarose aqueous solution forms a gel when the temperature drops to 35-45°C; Cover the scion and rootstock with plastic ...
Example Embodiment
[0037] Example 2
[0038] The method is the same as in Example 1, the difference is:
[0039] (1) Soak the scion in a carbendazim solution with a mass concentration of 0.2% for at least 1 hour;
[0040] (2) Split the prepared rootstock into a 1.2cm incision;
[0041] (3) Drop the agarose aqueous solution at a temperature of 44°C into the incision to make the agarose gel cover the incision; the mass concentration of the agarose aqueous solution is 0.3%; place the scion and rootstock at a temperature of 30°C and a humidity of 85~ 88% of greenhouses;
[0042] (4) The survival rate of jatropha transgenic seedlings is 92%.
Example Embodiment
[0043] Example 3
[0044] The method is the same as in Example 1, the difference is:
[0045] (1) Soak the scion in a 0.3% carbendazim solution for at least 1 hour;
[0046] (2) Split the prepared rootstock with a 1.5cm incision;
[0047] (3) Drop the agarose aqueous solution at a temperature of 43°C into the incision to make the agarose gel cover the incision; the mass concentration of the agarose aqueous solution is 0.3%; place the scion and rootstock at a temperature of 35°C and a humidity of 87~ 90% of greenhouses;
[0048] (4) The survival rate of jatropha transgenic seedlings was 93%.
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