Humic acid culture medium for improving growth speed of dendrobium candidum young stems and preparation method thereof
A technology of growth rate and dendrobium candidum, which is applied in the field of humic acid medium and its preparation to improve the growth rate of dendrobium candidum tender stems, can solve the problems of slow growth rate of dendrobium candidum, increase density and growth rate, and improve absorption efficiency , Improve the effect of chlorophyll content
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
preparation example Construction
[0094] The present invention also discloses a preparation method of a humic acid culture medium for improving the growth rate of dendrobium candidum tender stems, comprising the following steps:
[0095] S1, add sucrose, humic acid, macroelements, microelements, iron salts and organic matter to 5-7 times the amount of water at room temperature and mix to obtain product A;
[0096] S2, predissolve 2, 4-D and NAA in absolute ethanol to prepare a 0.5-1mg / ml solution to obtain product B;
[0097] S3, mix products A and B in tissue culture bottles, sterilize under high-temperature steam for 20-30 minutes, and cool to obtain.
[0098] Concrete formula embodiment is as follows:
Embodiment 1
[0100] Formula: sucrose 20g / L, potassium nitrate 50mg / L, ammonium nitrate 80.04mg / L, magnesium sulfate 246.47mg / L, calcium chloride 147.02mg / L, potassium iodide 166.01mg / L, boric acid 61.83mg / L, manganese sulfate 223.01 mg / L, Zinc Sulfate 287.54mg / L, Sodium Molybdate 241.95mg / L, Copper Sulfate 249.68mg / L, Cobalt Chloride 237.93mg / L, Disodium EDTA 372.25mg / L, Ferrous Sulfate 278.03 mg / L, inositol 100mg / L, glycine 2mg / L, thiamine hydrochloride 0.1mg / L, pyridoxine hydrochloride 0.5mg / L, niacin 0.5mg / L, potassium dihydrogen phosphate 75mg / L, humic acid Acid 0.5g / L, 2,4-D 2mg / L, NAA 0.5mg / L.
Embodiment 2
[0102] Formula: sucrose 20g / L, potassium nitrate 50mg / L, ammonium nitrate 80.04mg / L, magnesium sulfate 246.47mg / L, calcium chloride 147.02mg / L, potassium iodide 166.01mg / L, boric acid 61.83mg / L, manganese sulfate 223.01 mg / L, Zinc Sulfate 287.54mg / L, Sodium Molybdate 241.95mg / L, Copper Sulfate 249.68mg / L, Cobalt Chloride 237.93mg / L, Disodium EDTA 372.25mg / L, Ferrous Sulfate 278.03 mg / L, inositol 100mg / L, glycine 2mg / L, thiamine hydrochloride 0.1mg / L, pyridoxine hydrochloride 0.5mg / L, niacin 0.5mg / L, potassium dihydrogen phosphate 75mg / L, humic acid Acid 1g / L, 2,4-D 2mg / L, NAA 0.5mg / L.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com