Humic acid culture medium for improving growth speed of dendrobium candidum young stems and preparation method thereof

A technology of growth rate and dendrobium candidum, which is applied in the field of humic acid medium and its preparation to improve the growth rate of dendrobium candidum tender stems, can solve the problems of slow growth rate of dendrobium candidum, increase density and growth rate, and improve absorption efficiency , Improve the effect of chlorophyll content

Inactive Publication Date: 2019-04-02
苏州普朗特尔皮肤细胞科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, first of all, these mediums are all aimed at the growth of Dendrobium candidum, which belong to soil culture medium, and there is no liquid medium specifically for the growth of tender stems of Dendrobium candidum; secondly, because the growth rate of Dendrobium candidum itself is slow, how to increase the growth rate is also a matter of research and development. A direction pursued by personnel

Method used

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preparation example Construction

[0094] The present invention also discloses a preparation method of a humic acid culture medium for improving the growth rate of dendrobium candidum tender stems, comprising the following steps:

[0095] S1, add sucrose, humic acid, macroelements, microelements, iron salts and organic matter to 5-7 times the amount of water at room temperature and mix to obtain product A;

[0096] S2, predissolve 2, 4-D and NAA in absolute ethanol to prepare a 0.5-1mg / ml solution to obtain product B;

[0097] S3, mix products A and B in tissue culture bottles, sterilize under high-temperature steam for 20-30 minutes, and cool to obtain.

[0098] Concrete formula embodiment is as follows:

Embodiment 1

[0100] Formula: sucrose 20g / L, potassium nitrate 50mg / L, ammonium nitrate 80.04mg / L, magnesium sulfate 246.47mg / L, calcium chloride 147.02mg / L, potassium iodide 166.01mg / L, boric acid 61.83mg / L, manganese sulfate 223.01 mg / L, Zinc Sulfate 287.54mg / L, Sodium Molybdate 241.95mg / L, Copper Sulfate 249.68mg / L, Cobalt Chloride 237.93mg / L, Disodium EDTA 372.25mg / L, Ferrous Sulfate 278.03 mg / L, inositol 100mg / L, glycine 2mg / L, thiamine hydrochloride 0.1mg / L, pyridoxine hydrochloride 0.5mg / L, niacin 0.5mg / L, potassium dihydrogen phosphate 75mg / L, humic acid Acid 0.5g / L, 2,4-D 2mg / L, NAA 0.5mg / L.

Embodiment 2

[0102] Formula: sucrose 20g / L, potassium nitrate 50mg / L, ammonium nitrate 80.04mg / L, magnesium sulfate 246.47mg / L, calcium chloride 147.02mg / L, potassium iodide 166.01mg / L, boric acid 61.83mg / L, manganese sulfate 223.01 mg / L, Zinc Sulfate 287.54mg / L, Sodium Molybdate 241.95mg / L, Copper Sulfate 249.68mg / L, Cobalt Chloride 237.93mg / L, Disodium EDTA 372.25mg / L, Ferrous Sulfate 278.03 mg / L, inositol 100mg / L, glycine 2mg / L, thiamine hydrochloride 0.1mg / L, pyridoxine hydrochloride 0.5mg / L, niacin 0.5mg / L, potassium dihydrogen phosphate 75mg / L, humic acid Acid 1g / L, 2,4-D 2mg / L, NAA 0.5mg / L.

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Abstract

The invention discloses a humic acid culture medium for improving growth speed of dendrobium candidum young stems and a preparation method thereof. The preparation method comprises the following steps: adding humic acid and other nutritional substances into water at a room temperature, and uniformly mixing; and pre-dissolving a growth regulator by using absolute ethyl alcohol, uniformly mixing ina tissue culture bottle, sterilizing by high-temperature steam, and cooling, thus obtaining the humic acid culture medium. By adding and controlling the concentration of the humic acid, the metabolicactivity of plants is regulated, the content of chlorophyll is increased, and the density and growth rate can be increased.

Description

technical field [0001] The invention relates to the field of agricultural planting, in particular to a humic acid medium for improving the growth rate of the tender stems of Dendrobium officinale and a preparation method thereof. Background technique [0002] Dendrobium officinale is a perennial herbaceous Chinese herbal plant belonging to the genus Angiosperm, Liliaceae, Liliaceae, Orchidaceae, and Dendrobium. It is recorded in the Chinese Pharmacopoeia (2015 edition) that Dendrobium candidum has the effects of clearing heat and purging fire, detoxifying, diuresis and swelling, treating fever and polydipsia, sore throat, edema, oliguria, hot stranguria and pain, and carbuncle. Similarly, modern medical research shows that Dendrobium candidum has the function of enhancing immune T cells, which can improve human immunity, remove aging and mutated cells, stabilize blood sugar, increase cell metabolism rate, and maintain the physiological stability of the body. [0003] Wild D...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G24/10A01G24/20A01G24/30A01G31/00A01H4/00
CPCA01G24/10A01G24/20A01G24/30A01G31/00A01H4/001A01H4/008
Inventor 张杰王昕怡吴红升
Owner 苏州普朗特尔皮肤细胞科技有限公司
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