Characteristic spectrum construction method and quality detection method for bighead atractylodes rhizomes

A quality detection method and feature map technology, which are applied in the field of traditional Chinese medicine detection, can solve the problems of lack of targeted detection of effective components, poor pertinence and comprehensiveness of quality detection of Atractylodes Rhizoma, etc., to improve practical application value, easy to master, peak good shape

Inactive Publication Date: 2019-04-05
BEIJING ZHONGYAN TONGRENTANG CHINESE MEDICINE R & D
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] Therefore, the technical problem to be solved by the present invention is to overcome the defect that the fingerprint of Atractylodes Rhizome in the prior art does not carry out targeted detection and effectiveness detection on the active ingredients, thereby providing a method for constructing a feature map for the active ingredients of Atractylodes Rhizome,
[0007] Another technica...

Method used

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  • Characteristic spectrum construction method and quality detection method for bighead atractylodes rhizomes
  • Characteristic spectrum construction method and quality detection method for bighead atractylodes rhizomes
  • Characteristic spectrum construction method and quality detection method for bighead atractylodes rhizomes

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Embodiment 1

[0100] This embodiment provides a method for quality detection of Atractylodes Rhizoma Atractylodes Rhizome, including the method of constructing a characteristic map of Atractylodes Rhizoma Atractylodes Rhizome, and performing quality inspection on Atractylodes Rhizome products, including the following steps:

[0101] Preparation of test solution A: 6 samples of Atractylodes Rhizoma Atractylodes Rhizome, 6 samples of Atractylodes Rhizoma Atractylodes Rhizome and 6 samples of Bran-fried Atractylodes Rhizoma Rhizoma Rhizoma Rhizome produced in Zhejiang, Anhui, Hubei, Hunan, Hebei, Henan, 18 samples in total. into powder, passed through a No. 6 sieve, accurately weighed 0.5g, added 20ml of methanol (analytically pure), ultrasonicated for 40min, with a power of 250W and a frequency of 40KHZ, left to stand, to make up for the lost solvent, and to take the supernatant with a micropore size Filter through a filter membrane of 0.45um, and get the subsequent filtrate to obtain the test...

Embodiment 2

[0109] This embodiment provides a method for quality detection of Atractylodes Rhizoma Atractylodes Rhizome, including the method of constructing a characteristic map of Atractylodes Rhizoma Atractylodes Rhizome, and performing quality inspection on Atractylodes Rhizome products, including the following steps:

[0110] Preparation of the test solution: take the Atractylodes Rhizoma Atractylodes Rhizome to be tested and make it into powder, pass through a No. 6 sieve, weigh 0.3g accurately, add 20ml of n-hexane (analytical grade), ultrasonic 20min, the power is 300W, the frequency is 50KHZ , stand still, make up the lost solvent, take the supernatant and filter it with a filter membrane with a micropore size of 0.22um, and take the subsequent filtrate to obtain final product;

[0111] Preparation of reference substance solution: take Atractylodes macrocephala reference medicinal material to make powder, pass through a No. 6 sieve, accurately weigh 0.3g, add 20ml of n-hexane (ana...

Embodiment 3

[0115] This embodiment provides a method for quality detection of Atractylodes Rhizoma Atractylodes Rhizome, including the method of constructing a characteristic map of Atractylodes Rhizoma Atractylodes Rhizome, and performing quality inspection on Atractylodes Rhizome products, including the following steps:

[0116] Preparation of the test solution: take the fried Atractylodes Rhizoma Atractylodes Rhizoma Rhizome to be tested from Zhejiang and make powder, pass through a No. 6 sieve, accurately weigh 0.8g, add methanol (60% by volume) 25ml, cold soak for 60min, and let it stand , make up the lost solvent, take the supernatant and filter it with a filter membrane with a micropore size of 0.45um, and get the continued filtrate to obtain final product;

[0117] Preparation of reference solution: take Atractylodes macrocephala reference medicinal material to make powder, pass through a No. 6 sieve, accurately weigh 0.8g, add 25ml of methanol (60% by volume), cold soak for 60min,...

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Abstract

The invention relates to the field of traditional Chinese medicine detection technology, in particular to a characteristic spectrum construction method and a quality detection method for bighead atractylodes rhizomes. According to the characteristic spectrum construction method for bighead atractylodes rhizomes, a characteristic spectrum of effective ingredients, namely atractylenolide I, atractylenolide II, atractylenolide III and atractylon of bighead atractylodes rhizome medicinal materials, bighead atractylodes rhizome herbal pieces and bighead atractylodes rhizome herbal pieces stir-bakedwith bran are analyzed through efficient liquid chromatographic analysis, chromatographic peaks of the characteristic spectrum are completely displayed, uniformity is good, the characteristic chromatographic peaks are completely separated, the quantity of impurities is small, a contrast characteristic spectrum is established according to the characteristic spectrum, and meanwhile the content of atractylenolide I and the content of atractylenolide III are determined. In combination with thin-layer chromatography identification and heavy metal and harmful element determination, the comprehensive quality detection method for bighead atractylodes rhizomes with effectiveness and safety is provided, so that quality detection on bighead atractylodes rhizomes, processed products of bighead atractylodes rhizomes and relevant products is realized. Moreover, the quality detection method has the advantages of being simple, fast, stable, reliable, high in precision, good in reproducibility, easy to grasp and the like.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and in particular relates to a method for constructing a characteristic map of Atractylodes macrocephala and a method for quality detection. Background technique [0002] Atractylodes macrocephala is the dried rhizome of Atractylodes macrocephala Koidz, a plant in the family Compositae. It is a commonly used tonic traditional Chinese medicine in my country. [0003] With the enhancement of people's awareness of health care, people have higher requirements for the quality and safety of traditional Chinese medicine products. At present, the quality standards of Atractylodes macrocephala include simple properties, identification, routine inspection, and determination of extracts, as well as the determination of HPLC fingerprints. Traditional Chinese medicine fingerprint is a comprehensive and quantifiable means of identification, and it is one of the current quality c...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 姚璐王淑英王海张润李晩婧颜士华
Owner BEIJING ZHONGYAN TONGRENTANG CHINESE MEDICINE R & D
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