Application of ilexin B in preparation of medicine for treating IBD (inflammatory bowel disease)
A technology for inflammatory bowel disease and glucoside, which is applied in the field of application of glucoside B in the preparation of drugs for treating inflammatory bowel disease, and can solve problems such as elevated lipase levels, high fever, and elevated transaminases
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Embodiment 1
[0022] The preparation method of glucoside B
[0023] (1) Extraction: Obtained by relying on the preparation method of the root extract of G. gongguo (see patent: ZL201110314778.6 for the specific method).
[0024] (2) Separation by silica gel column: take the root extract of G. quinquefolius, dissolve it in an equal amount of methanol, add 60-100 mesh silica gel in a ratio of 1.5--2.5 times the amount, mix the sample under reduced pressure until dry, load the sample by dry method, and use 10- 20 times the amount of silica gel column chromatography, with dichloromethane-methanol volume ratio of 95:5, 93:7, 90:10 gradient elution, half the column bed volume as a receiving volume, by thin layer chromatography and Combined with analytical HPLC detection, collect the components rich in glucoside B, combine them, and concentrate them to dryness under reduced pressure at a temperature ≤ 60°C and a vacuum degree of 0.06-0.08 MPa to obtain a glucoside B enrichment with a content of 1...
Embodiment 2
[0034] Effect of glucoside B on experimental colopathy induced by dextran sulfate sodium in mice.
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