Mouse liver primary cell culture medium, culture method and incubator

Abstract

The invention provides a mouse liver primary cell culture medium and a culture method. The formula of the culture medium comprises a William's E culture medium, bovine serum albumin, insulin, hexadecadrol, L-glutamine, penicillin and streptomycin. The culture method comprises the following step of culturing mouse liver primary cells through the mouse liver primary cell culture medium. The culturemedium disclosed by the invention can completely meet the growth requirements of primary liver cells, the growth state of the cells is good, and the culture medium is simple to compound and low in cost and has the advantages of being quick, economic and efficient. According to the culture method disclosed by the invention, fetal calf serum is not used in the all stages, so that influence of complex components of the serum on the mouse primary liver cells can be avoided. The invention further provides an improved cell culture incubator.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a culture medium for mouse liver primary cells, a culture method and an incubator used in the culture method. Background technique [0002] Primary cultured hepatocytes derived from the liver and prepared by a special separation method are called primary hepatocytes. The mouse liver was removed from the body, treated with trypsin and chelating agent (commonly used EDTA), dispersed into single cells, and the cells prepared from liver tissue were mainly hepatocytes, but there were also bile duct epithelial cells, astrocytes, Hepatic sinusoidal endothelial cells, blood cells, etc. need to be removed, screened through a mesh of a certain size, centrifuged at a suitable speed, and placed in a suitable medium for cultivation and screening, and finally high-purity hepatocytes can be obtained. [0003] High-purity primary liver cells are widely used in the biological experiments of the live...

AI Technical Summary

Problems solved by technology

[0005] Existing problems: HepatoZYME-SFM serum-free medium is expensive and used in large quantities, which causes a lot of inconvenience in the cultivation of primary liver cells

[0008] However, there is no culture medium and culture method that does not use fetal calf serum to cultivate mouse liver primary cells and can ensure good cell growth.

[0009] At the same time, when cell culture is carried out, the convenient use of the incubator is also conducive to the smooth progress of cell culture. Currently, the existing biological cell incubators can only place petri dishes or culture bottles with a fixed height, and the scope of application is limited. For example, the patent CN203462058U, 2014.03.05, a biological cell culture device was disclosed. The biological cell culture device can use the detachable clamps on the column. The user can adjust the distance between the clamps at will, and can place cultures of any size. However, in actual use, since the device is not convenient for experimental operators to place and take out the culture medium or culture dish, it is prone to collision, resulting in damage or contamination of the culture dish, etc. Improvement, therefore the present invention also proposes an improved cell culture box for solving the above problems

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