Method for accurately identifying disease resistance of newly-planted sugarcane white leaf

Abstract

The invention provides a method for accurately identifying disease resistance of newly-planted sugarcane white leaf. The method comprises: cutting sugarcane stalks infected with sugarcane white leaf phytoplasma, performing juicing, adding 10 times amount of sterile water to prepare an inoculating liquid; cutting the sugarcane stalks which are a sugarcane material to be tested into stalk sections with buds, soaking the sections in running tap water for 48h, performing hot water treatment at 50 DEG C for 2h, and performing soaking with an insecticidal and sterilizing agent for 10min; performingcoating inoculation with a plastic film, and performing moisture preservation at 25 DEG C for 24h; planting an inoculating material in a plastic barrel, and placing the barrel in a 20-30 DEG C insecticidal greenhouse for culture; and investigating infected plant rate, and performing disease resistance evaluation according to 1-5 grade standards. The method of the invention firstly establishes a set of accurate and efficient method for identifying disease resistance of newly-planted sugarcane white leaf, and provides technical support for sugarcane white leaf resistance breeding. The pathogen inoculating liquid is sprayed onto the sugarcane seed and the coating inoculation with a plastic film is performed, so that a disease habitat condition is provided, and the result is objective, true and reliable. The inoculation is quick and simple, the operability is strong, and the work efficiency is high. After the inoculation, the sugarcane white leaf has obvious incidence, high sensitivity andgood reproducibility.

Description

technical field [0001] The invention belongs to the technical field of crop disease resistance identification, and in particular relates to an accurate identification method for newly planted sugarcane white leaf disease resistance. Background technique [0002] Sugarcane white leaf (SCWL) is the first important new disease of quarantine risk caused by Sugarcane white leaf phytoplasma on sugarcane in my country. It spreads very rapidly and seriously threatens sugarcane in China. production and development. Sugarcane white leaf disease was first discovered in Lampang, northern Thailand in 1954, and has now widely occurred in Southeast Asian countries such as India, Pakistan, Sri Lanka, Japan, Laos, Myanmar, Vietnam, and the Philippines. Sugarcane white leaf phytoplasma (Sugarcane white leaf phytoplasma) is a prokaryotic microorganism without a cell wall, which cannot be cultured in vitro in an artificial medium. The incubation period is long, the shortest is about 30 days (a ...

AI Technical Summary

Problems solved by technology

Sugarcane white leaf disease is a new disease that has rapidly expanded in recent years. So far, no method for identification of sugarcane white leaf disease resistance has been established.

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