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Electrochemical sensor for detecting lead ion and manufacturing method thereof

An electrochemical and lead ion technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of high cost, low specificity and sensitivity

Active Publication Date: 2019-04-16
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of low specificity and sensitivity and high cost of the method for detecting lead ions in the above prior art, the present invention provides a CHA amplification method based on DNase with high specificity and sensitivity, low cost and fast detection speed. Electrochemical sensor for detection of lead ions connected with Zenghe nano-gold

Method used

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  • Electrochemical sensor for detecting lead ion and manufacturing method thereof
  • Electrochemical sensor for detecting lead ion and manufacturing method thereof
  • Electrochemical sensor for detecting lead ion and manufacturing method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0059] A preparation method of the electrochemical biosensor of the present invention:

[0060] a. The gold electrode is first polished in 0.3 and 0.05 µm alumina slurry until it becomes a mirror surface, and then rinsed repeatedly with PBS and secondary water;

[0061] b. Add 10 μL of 1 μM CP2 dropwise to the surface of the electrode through the Au-S bond, incubate at room temperature for 2 h, and wash.

[0062] So far, the modification process of the electrode has come to an end. The following describes the reaction in the homogeneous solution and the main steps in the homogeneous reaction:

[0063] a. Mix 1X PBS buffer, (0 μM, 0.5 μM, 1 μM, 1.5 μM, 2 μM) HP1, 1 μM HP2, 1 μM HP3, 3 μM SP-AuNP (1 nM), 1 μM DP and the Add 2 μL of each target substance and 6 μL of sterilized water into the centrifuge tube, shake for 30 s, and incubate in a 37 °C incubator for 2 h;

[0064] b. Add the solution (10 μL) after the reaction in step a dropwise onto the pre-modified CP2 electrode. ...

Embodiment 2

[0071] A preparation method of the electrochemical biosensor of the present invention:

[0072] a. The gold electrode is first polished in 0.3 and 0.05 µm alumina slurry until it becomes a mirror surface, and then rinsed repeatedly with PBS and secondary water;

[0073] b. Add 10 μL (0 μM, 0.5 μM, 1 μM, 1.5 μM, 2 μM) of CP2 dropwise onto the electrode surface and incubate at room temperature for 2 hours. Fix the CP2 chain to the electrode surface through the Au-S bond and wash;

[0074] So far, the modification process of the electrode has come to an end. The following describes the reaction in the homogeneous solution and the main steps in the homogeneous reaction:

[0075] a. Add 1X PBS buffer, 1 μM HP1, 1 μM HP2, 1 μM HP3, 3 μM SP-AuNP (1 nM), 1 μM DP and 2 μL of the target to be tested, and 6 μL of sterile water into the centrifuge tube , shake for 30 s, and incubate in a 37°C incubator for 2 h;

[0076] b. Add the solution (10 μL) after the reaction in step a dropwise ...

Embodiment 3

[0083] A preparation method of the electrochemical biosensor of the present invention:

[0084] a. The gold electrode is first polished in 0.3 and 0.05 µm alumina slurry until it becomes a mirror surface, and then rinsed repeatedly with PBS and secondary water;

[0085] b. Add 10 μL of 1 μM CP2 dropwise to the electrode surface and incubate at room temperature for 2 h. Fix the CP2 chain to the electrode surface through the Au-S bond and wash;

[0086] So far, the modification process of the electrode has come to an end. The following describes the reaction in the homogeneous solution and the main steps in the homogeneous reaction:

[0087] a. Mix 1X PBS buffer, 1 μM HP1, 1 μM HP2, 1 μM HP3, (1.2 μM, 2.1 μM, 3 μM, 3.9 μM, 4.8 μM) SP-AuNP (0.4 nM, 0.7 nM, 1 nM, 1.3 nM, 1.6 nM), 1 μM DP and 2 μL of the target to be tested, and 6 μL of sterilized water were added to the centrifuge tube, shaken for 30 s, and incubated in a 37 °C incubator for 2 h; Each concentration corresponds ...

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Abstract

The invention relates to the electrochemical sensor technology field and especially relates to an electrochemical sensor for detecting a lead ion. A CP2 layer, a HP1-HP2-HP3-CP1-AuNP layer, and a [Ru(NH3)6]<3+> layer are sequentially modified on an electrode. The manufacturing method comprises the following steps of preprocessing the electrode; modifying the CP2 layer to an electrode surface; modifying the HP1-HP2-HP3-CP1-AuNP layer to the electrode surface; and modifying the [Ru(NH3)6]<3+> layer to the electrode surface. The specific identification of a DNA enzyme is used to realize the highspecificity detection of target object lead ions. The cutting effect of the DNA enzyme of lead ion specific identification and a CHA reaction are used to realize the increase of a signal through two-step cycle and one-step amplification modes so as to realize the high-sensitivity detection of lead ions and obtain a lower detection limit. Through the connection performance of nanogold, an effect that the signal is continuously enhanced is achieved.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensors, in particular to an electrochemical sensor for detecting lead ions and a preparation method thereof. Background technique [0002] As a well-known and non-degradable environmental pollutant, lead ions can be enriched in the human body through the food chain, and at the same time cause harm to the immune system, central nervous system, and reproductive system, especially for children. Therefore, it is urgent and important to construct an effective analytical method capable of detecting trace lead ions in the environment. [0003] At present, commonly used lead ion detection methods include atomic absorption spectrometry, atomic emission spectrometry, inductively coupled plasma mass spectrometry, X-ray fluorescence spectrometry, etc. These methods often have the disadvantages of expensive instruments, long analysis period, complicated sample pretreatment, Problems such as high dete...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 刘素宋晓蕾黄加栋王玉张雪王敬锋王海旺
Owner UNIV OF JINAN
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