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Application of polydopamine-modified silver nanoparticle in mass spectrometry detection

A silver nanoparticle and nanoparticle technology, applied in the field of mass spectrometry detection, to achieve the effect of enhancing desorption ionization ability, simple operation and broad application value

Active Publication Date: 2019-04-16
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The matrix can be applied to both positive and negative ion mode detection of MALDI to solve the problem of MALDI mass spectrometry analysis of low-abundance lipids

Method used

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  • Application of polydopamine-modified silver nanoparticle in mass spectrometry detection
  • Application of polydopamine-modified silver nanoparticle in mass spectrometry detection
  • Application of polydopamine-modified silver nanoparticle in mass spectrometry detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1. Preparation of AgNPs@PDA nanoparticles

[0044] Weigh 0.4g of dopamine and dissolve it in a round bottom flask containing 25mL of water, and adjust the pH to 7.8.

[0045] Prepare 0.01g / mL silver nitrate solution (the solvent is water), slowly drop 4mL into a round bottom flask, and collect samples after reacting at 60°C for 24 hours to obtain AgNPs@PDA nanoparticles. If the dripping method is not used, the obtained AgNPs are coral-like materials, such as figure 1 shown in a.

[0046] The TEM image of the prepared AgNPs@PDA nanoparticles is as follows figure 1 As shown in b, 1c, it can be seen from the figure that the AgNPs@PDA particles are spherical with a diameter between 85-110nm, the thickness of polydopamine is about 6.5nm, and the surface of polydopamine is attached with 3-5nm AgNPs, which can be clearly seen Lattice lines of silver.

[0047] AgNPs@PDA nanoparticles have good dispersibility in water, methanol, acetonitrile and other solvents, and can be evenl...

Embodiment 2

[0048] Example 2. Using AgNPs@PDA as the MALDI matrix to detect PA (18:0 / 18:0), the example uses AgNPs@PDA's own silver cluster peak calibration instrument.

[0049] Matrix dispersion: Weigh 2 mg of AgNPs@PDA particles prepared in Example 1 and dissolve in 1 mL of acetonitrile / water (80 / 20, v / v), and ultrasonically disperse for 20 minutes.

[0050] The preparation concentration is 10 -3 M phosphatidic acid PA (18:0 / 18:0) standard solution.

[0051] The matrix dispersion and the prepared standard solution were mixed according to the volume ratio of 1:1, and 2μL was spotted on the MALDI stainless steel target surface and dried at room temperature; sent to the mass spectrometer for analysis.

[0052] Mass spectrometry conditions are: equipped with a 9.4T superconducting magnet Bruker solariX mass spectrometer, laser wavelength 355nm, laser energy 75%, laser spot size 50μm, pulse frequency 150Hz, resolution 200m / z at 200m / z.

[0053] Test results such as figure 2 As shown, it can be seen f...

Embodiment 3

[0055] Example 3. Using AgNPs@PDA as a substrate to detect mouse brain lipid extract in positive ion mode

[0056] Refer to Example 2 for the preparation method of the matrix dispersion.

[0057] Lipid extraction: prepare mouse brain homogenate (200mg / mL), add 50μL of homogenate to 450μL of methanol, vortex for 1min, let stand for 5min, then vortex for 1min, and centrifuge for 10min in a centrifuge (1000g, 4℃) ). The supernatant was collected, dried with nitrogen, and re-dissolved in 200 μL of methanol.

[0058] The matrix solution and the re-dissolved lipid extract were mixed in a ratio of 1:1 (v / v), and then 2 μL was sampled on the MALDI stainless steel target surface with a pipette, dried at room temperature, and sent to the mass spectrometer for analysis.

[0059] Mass spectrometry conditions are: equipped with a 9.4T superconducting magnet Bruker solariX mass spectrometer, laser wavelength 355nm, laser energy 75%, laser spot size 50μm, pulse frequency 150Hz, resolution 200m / z at...

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Abstract

The invention discloses AgNPs@PDA nanoparticles and a preparation method thereof, and an application in mass spectrometry detection. The AgNPs@PDA nanoparticles are obtained through reducing AgNO3 bydopamine under an alkaline condition, and a form is a spherical type. The application range of a MALDI mass spectrum is extended and low-abundance lipids and metabolic small molecules in a biologicalsample can be detected. An AgNPs@PDA nanoparticle synthesis condition is mild and simple, non-toxic and harmless, operation in an MALDI mass spectrum test is simple and a high application value is possessed in the field of analysis and detection. The method is a simple and high-efficient method used for improving a mass spectrum detection capability and can be used for organism MALDI mass spectrumimaging analysis and the like.

Description

Technical field [0001] The invention belongs to the technical field of mass spectrometry detection, and specifically relates to an application of polydopamine modified silver nanoparticles (AgNPs@PDA) in mass spectrometry detection. Background technique [0002] Matrix-Assisted Laser Desorption / Ionization Mass Spectrometry (Matrix-Assisted Laser Desorption / Ionization Mass Spectrometry) is abbreviated as MALDI mass spectrometry, which is a new type of soft ionization biological mass spectrometry developed in recent years. Matrix-Assisted Laser Desorption / Ionization Mass Spectrometry Imaging (MALDI MSI) is a MALDI-based imaging technology, and it is also an important technology developed in recent years to study the distribution of components in biological tissues. The in-situ imaging analysis of components such as endogenous metabolic small molecules and exogenous drug molecules in the sample has the advantages of no labeling and high sensitivity. MALDI MSI has been widely used i...

Claims

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Application Information

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IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 韩超赵镇文杨慧张阳阳李娜
Owner INST OF CHEM CHINESE ACAD OF SCI
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