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Application of polydopamine-modified silver nanoparticles in mass spectrometry detection

A nanoparticle and polydopamine technology, which is applied in the field of mass spectrometry detection, achieves the effects of mild and convenient synthesis conditions, enhanced desorption and ionization ability, and simple operation

Active Publication Date: 2021-03-16
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The matrix can be applied to both positive and negative ion mode detection of MALDI to solve the problem of MALDI mass spectrometry analysis of low-abundance lipids

Method used

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  • Application of polydopamine-modified silver nanoparticles in mass spectrometry detection
  • Application of polydopamine-modified silver nanoparticles in mass spectrometry detection
  • Application of polydopamine-modified silver nanoparticles in mass spectrometry detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1, preparation of AgNPs@PDA nanoparticles

[0044] Weigh 0.4 g of dopamine and dissolve it in a round bottom flask filled with 25 mL of water, and adjust the pH value to 7.8.

[0045] Prepare 0.01g / mL silver nitrate solution (solvent is water), slowly drop 4mL into a round bottom flask, react at 60°C for 24 hours and collect samples to obtain AgNPs@PDA nanoparticles. If the dropping method is not used, the obtained AgNPs are coral-like materials, such as figure 1 as shown in a.

[0046] The transmission electron microscope images of the prepared AgNPs@PDA nanoparticles are as follows: figure 1 b, as shown in 1c, it can be seen from the figure that the AgNPs@PDA particles are spherical, with a diameter of 85-110nm, and the thickness of polydopamine is about 6.5nm. AgNPs of 3-5nm are attached to the surface of polydopamine, which can be clearly seen Silver lattice lines.

[0047] AgNPs@PDA nanoparticles have good dispersibility in water, methanol, acetonitrile...

Embodiment 2

[0048] Example 2. Using AgNPs@PDA as a MALDI matrix to detect PA (18:0 / 18:0), an example of using AgNPs@PDA's own silver cluster peak to calibrate the instrument.

[0049] Matrix dispersion liquid: Weigh 2 mg of the AgNPs@PDA particles prepared in Example 1 and dissolve in 1 mL of acetonitrile / water (80 / 20, v / v), and disperse ultrasonically for 20 min.

[0050] The preparation concentration is 10 -3 Phosphatidic acid PA (18:0 / 18:0) standard solution of M.

[0051] The matrix dispersion and the prepared standard solution were mixed according to the volume ratio of 1:1, and 2 μL was spotted on the MALDI stainless steel target surface, and dried at room temperature; then sent to a mass spectrometer for analysis.

[0052] The mass spectrometer conditions are: equipped with a 9.4T superconducting magnet Bruker solariX mass spectrometer, the laser wavelength is 355nm, the laser energy is 75%, the laser spot size is 50μm, the pulse frequency is 150Hz, and the resolution is 200000 at...

Embodiment 3

[0055] Example 3. Using AgNPs@PDA as a matrix to detect mouse brain lipid extracts in positive ion mode

[0056] Refer to Example 2 for the preparation method of matrix dispersion.

[0057] Lipid extraction: Prepare mouse brain homogenate (200mg / mL), take 50μL homogenate and add 450μL methanol, vortex for 1min, let stand for 5min, vortex for 1min, and centrifuge in a centrifuge for 10min (1000g, 4℃ ). The supernatant was collected, blown dry with nitrogen gas, and redissolved in 200 μL methanol.

[0058] The matrix solution and the re-dissolved lipid extract were mixed at a ratio of 1:1 (v / v), and then 2 μL was spotted on the MALDI stainless steel target surface with a pipette, dried at room temperature, and sent to a mass spectrometer for analysis.

[0059] The mass spectrometer conditions are: equipped with a 9.4T superconducting magnet Bruker solariX mass spectrometer, the laser wavelength is 355nm, the laser energy is 75%, the laser spot size is 50μm, the pulse frequency...

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Abstract

The invention discloses AgNPs@PDA nanoparticles and a preparation method thereof, and an application in mass spectrometry detection. The AgNPs@PDA nanoparticles are obtained through reducing AgNO3 bydopamine under an alkaline condition, and a form is a spherical type. The application range of a MALDI mass spectrum is extended and low-abundance lipids and metabolic small molecules in a biologicalsample can be detected. An AgNPs@PDA nanoparticle synthesis condition is mild and simple, non-toxic and harmless, operation in an MALDI mass spectrum test is simple and a high application value is possessed in the field of analysis and detection. The method is a simple and high-efficient method used for improving a mass spectrum detection capability and can be used for organism MALDI mass spectrumimaging analysis and the like.

Description

technical field [0001] The invention belongs to the technical field of mass spectrometry detection, and in particular relates to the application of polydopamine-modified silver nanoparticles (AgNPs@PDA) in mass spectrometry detection. Background technique [0002] Matrix-Assisted Laser Desorption / Ionization Mass Spectrometry (MALDI mass spectrometry) is a new type of soft ionization mass spectrometry developed in recent years. Matrix-Assisted Laser Desorption / Ionization Mass Spectrometry Imaging (MALDI MSI for short) is an imaging technology based on MALDI, and it is also an important technology developed in recent years to study the distribution of components in biological tissues. The in situ imaging analysis of components such as endogenous metabolic small molecules and exogenous drug molecules in the sample has the advantages of no labeling and high sensitivity. MALDI MSI has been widely used in the detection and analysis of various samples, especially the detection of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 韩超赵镇文杨慧张阳阳李娜
Owner INST OF CHEM CHINESE ACAD OF SCI
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