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The application of ang2 as an indicator molecule for the effect of sorafenib in the treatment of liver cancer

A sorafenib and liver cancer technology, applied in the field of genetic engineering, can solve the problems of limited therapeutic effect and difference in effect

Active Publication Date: 2022-04-01
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its therapeutic effect is limited. According to existing clinical trial reports, in the SHARP (Sorafenib Hepatocellular Carcinoma Assessment Randomized Protocol) trial, Sorafenib only increased the median overall survival time of liver cancer patients by 2.8 months; In a phase III clinical trial of advanced liver cancer, Sorafenib only improved the median overall survival time of patients by 2.3 months
In addition, the effect of sorafenib clinical treatment also has individual differences

Method used

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  • The application of ang2 as an indicator molecule for the effect of sorafenib in the treatment of liver cancer
  • The application of ang2 as an indicator molecule for the effect of sorafenib in the treatment of liver cancer
  • The application of ang2 as an indicator molecule for the effect of sorafenib in the treatment of liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Sarafini pair VETC + / Vetc - Analysis of therapeutic effects of patients with liver cancer

[0019] 1.1 Immunohistochemical detection of blood vessels in liver cancer

[0020] Slices of human liver cancer tissue are provided by the subscript library of the Affiliated Cancer Hospital of Sun Yat-sen University. Made of paraffin slice 4 ° C. The follow-up test step is:

[0021] (1) Preprocessing: Take out of 55 ° C baking sheet for 1 h, placed in xylene twice, 10 min each time; use 100%, 95%, 90%, 80%, 70% ethanol, each step 3min, was washed with double steamer; 0.3% of the hydrogen peroxide treatment for 10min, double steamed 3 min, 3 minutes each time; soak in the repair liquid, heat repair with a microwave oven, room temperature is half an hour; use 1 × PBS wash Three times, 3 min each time;

[0022] (2) Hybrid staining: Incubate with CD34 antibody, overnight at 4 ° C; restore room temperature, discard the antibody fluid, wash three times with 1 × PBS, 5 minutes ...

Embodiment 2

[0029] Example 2: Human organizational specimen Ang2 expression indicates blood vessel type of liver cancer

[0030] 2.1 Immunohistochemical detection

[0031]Slices of human liver cancer tissue are provided by the subscript library of the Affiliated Cancer Hospital of Sun Yat-sen University. Made of paraffin slice 4 ° C. The follow-up test step is:

[0032] (1) Preprocessing: Take out of 55 ° C baking sheet for 1 h, placed in xylene twice, 10 min each time; use 100%, 95%, 90%, 80%, 70% ethanol, each step 3min, was washed with double steamer; 0.3% of the hydrogen peroxide treatment for 10min, double steamed 3 min, 3 minutes each time; soak in the repair liquid, heat repair with a microwave oven, room temperature is half an hour; use 1 × PBS wash Three times, 3 min each time;

[0033] (2) Hybrid staining: incubation with Ang2 antibody, overnight 4 ° C; restore room temperature, discard the antibody fluid, wash three times with 1 × PBS, 5 minutes each time; add secondary antibiotic,...

Embodiment 3

[0046] A reagent for detecting VETC vascular type liver cancer is provided, which contains a specific antibody or probe of Ang2. When the reagent was used to detect the type of liver cancer, the blood of the patient was extracted, and the reagent was applied to the patient's plasma or serum, and then the expression of Ang2 in patient plasma or serum was detected.

[0047] If the expression of Ang2 is higher than a value, it can be initially judged that the patient may have VETC vascular type liver cancer. In order to diagnose, further detection can be carried out; if the expression amount of Ang2 is below a certain value, it can be initially judged, the patient The liver cancer may not be VETC vascular type liver cancer; further detection can be carried out in order to diagnose.

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Abstract

The invention relates to the application of sorafenib in the treatment of VETC blood vessel type liver cancer drugs, and the application of ang2 as an indicator molecule for the effect of sorafenib in treating liver cancer. The applicant discovered a new type of liver cancer, that is, VETC vascular liver cancer, and found that Sorafenib has a significant therapeutic effect on VETC vascular liver cancer. At the same time, the applicant found that the expression of ang2 in VETC vascular type liver cancer is high. Then, by detecting the expression of ang2 in the blood of patients with liver cancer, we can preliminarily judge whether the patient's cancer is VETC vascular type liver cancer, and also predict the therapeutic effect of sorafenib on liver cancer.

Description

Technical field [0001] The present invention belongs to the field of gene engineering, and specifically, the application of Ang2 as an indication molecule for the treatment of liver cancer effects in Sarafini. Background technique [0002] Hepatocellular Carcinoma (HCC) is one of the most common malignant tumors in the world, and its incidence and mortality are ranked in front of various malignant tumors. China is a high-risk area of ​​liver cancer, accounting for 55% of the total number of HCC patients around the world. It is well known that the main cause of cancer is difficult to cure is local recurrence and distant transfer, cancer cell metastasis is the main reason for tumor recurrence. It is generally believed that the liver rich vascular system and the active angiogenesis of liver cancer are the factors that are susceptible to blood transfer in patients with liver cancer, especially an important factor in early liver spread. However, existing studies have shown controversy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574A61K31/44A61P35/00A61P1/16
CPCA61P1/16A61P35/00G01N33/57438G01N33/57484A61K31/44G01N2333/515
Inventor 庄诗美方坚鸿郑利民
Owner SUN YAT SEN UNIV