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Application of ang2 as indicator molecule of treatment effect of sorafenib on liver cancer

A sorafenib and liver cancer technology, applied in the field of genetic engineering, can solve the problems of limited therapeutic effect and difference in effect

Active Publication Date: 2019-04-16
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its therapeutic effect is limited. According to existing clinical trial reports, in the SHARP (Sorafenib Hepatocellular Carcinoma Assessment Randomized Protocol) trial, Sorafenib only increased the median overall survival time of liver cancer patients by 2.8 months; In a phase III clinical trial of advanced liver cancer, Sorafenib only improved the median overall survival time of patients by 2.3 months
In addition, the effect of sorafenib clinical treatment also has individual differences

Method used

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  • Application of ang2 as indicator molecule of treatment effect of sorafenib on liver cancer
  • Application of ang2 as indicator molecule of treatment effect of sorafenib on liver cancer
  • Application of ang2 as indicator molecule of treatment effect of sorafenib on liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 Sorafenib to VETC + / VETC - Analysis of curative effect in patients with liver cancer

[0019] 1.1 Immunohistochemical detection of blood vessel types in liver cancer tissue

[0020] The slices of human liver cancer tissue were provided by the Specimen Bank of Cancer Hospital Affiliated to Sun Yat-sen University. Paraffin sections were made and stored at 4°C. Subsequent detection steps are:

[0021] (1) Pretreatment: Take out the 55°C baking sheet for 1 hour, put it in xylene to dewax twice, 10 minutes each time; wash it once with 100%, 95%, 90%, 80%, and 70% ethanol in turn, and wash it once with each step After 3 minutes, wash with double distilled water for 3 minutes; treat with 0.3% hydrogen peroxide for 10 minutes, wash with double distilled water for 3 times, each time for 3 minutes; soak in repair solution, heat repair with microwave oven, cool at room temperature for half an hour; wash with 1×PBS Three times, 3 minutes each time;

[0022] (2) ...

Embodiment 2

[0029] Example 2: The expression of ang2 in human tissue samples indicates the blood vessel type of liver cancer tissue

[0030] 2.1 Immunohistochemical detection

[0031]The slices of human liver cancer tissue were provided by the Specimen Bank of Cancer Hospital Affiliated to Sun Yat-sen University. Paraffin sections were made and stored at 4°C. Subsequent detection steps are:

[0032] (1) Pretreatment: Take out the 55°C baking sheet for 1 hour, put it in xylene to dewax twice, 10 minutes each time; wash it once with 100%, 95%, 90%, 80%, and 70% ethanol in turn, and wash it once with each step After 3 minutes, wash with double distilled water for 3 minutes; treat with 0.3% hydrogen peroxide for 10 minutes, wash with double distilled water for 3 times, each time for 3 minutes; soak in repair solution, heat repair with microwave oven, cool at room temperature for half an hour; wash with 1×PBS Three times, 3 minutes each time;

[0033] (2) Hybridization and staining: Incuba...

Embodiment 3

[0046] The invention provides a reagent for detecting VETC blood vessel type liver cancer, which contains the specific antibody or probe of ang2. When using the reagent to detect the type of liver cancer, the patient's blood is drawn, the reagent is applied to the patient's plasma or serum, and then the expression level of ang2 in the patient's plasma or serum is detected.

[0047] If the expression level of ang2 is higher than a certain value, it can be preliminarily judged that the patient may suffer from VETC vascular type liver cancer, and further testing can be carried out for diagnosis; The HCC may not be VETC vascular type HCC; further testing may be done to confirm the diagnosis.

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PUM

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Abstract

The invention relates to an application of sorafenib in treatment drugs for VETC vascular type liver cancer, and an application of ang2 as an indicator molecule of a treatment effect of sorafenib on liver cancer. The applicant finds a new type of liver cancer, namely the VETC vascular type liver cancer, and finds that the sorafenib has a remarkable treatment effect on the VETC vascular type livercancer. Meanwhile, the applicant finds that the expression quantity of ang2 is high in the VETC vascular type liver cancer. Through the detection of the ang2 expression quantity in blood of a patientsuffering from the liver cancer, whether the cancer suffered by the patient is the VETC vascular type liver cancer or not can be preliminarily judged, and the treatment effect of the sorafenib on theliver cancer can also be pre-judged.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and specifically relates to the application of ang2 as an indicator molecule for the effect of sorafenib in treating liver cancer. Background technique [0002] Primary hepatocellular carcinoma (hepatocellular carcinoma, HCC) is one of the most common malignant tumors in the world, and its incidence rate and mortality rate are among the highest in various malignant tumors. China is a high-incidence area for liver cancer, accounting for 55% of the total number of HCC patients in the world. As we all know, the main reasons why cancer is difficult to cure are local recurrence and distant metastasis, and cancer cell metastasis is the main reason for tumor recurrence. It is generally believed that the rich vascular system of the liver and the active angiogenesis of liver cancer are factors that are prone to hematogenous metastasis in patients with liver cancer, especially important factors for earl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574A61K31/44A61P35/00A61P1/16
CPCA61P1/16A61P35/00G01N33/57438G01N33/57484A61K31/44G01N2333/515
Inventor 庄诗美方坚鸿郑利民
Owner SUN YAT SEN UNIV