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Endopleura-containing in-vitro embryo culture method for determining vitality of seeds of xanthoceras sorbifolia

Active Publication Date: 2019-04-23
HEXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the special dormancy characteristics of X. sorbifolium seeds, the seed coat is hard, and the existing TTC dyeing method, red ink dyeing method, bromomusmol blue dyeing method, indigo carmine dyeing method and isolated embryo culture method cannot be used for X. sorbifolium seedlings. Viability is measured accurately and with poor reproducibility

Method used

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  • Endopleura-containing in-vitro embryo culture method for determining vitality of seeds of xanthoceras sorbifolia
  • Endopleura-containing in-vitro embryo culture method for determining vitality of seeds of xanthoceras sorbifolia
  • Endopleura-containing in-vitro embryo culture method for determining vitality of seeds of xanthoceras sorbifolia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for culturing isolated seed coat in vitro embryos of rare woody economic crops Xantho sorbifolium seed viability, the batch of seeds comes from the Xantho sorbifolium base of the Qilian Mountain Water Conservation Forest Research Institute in Zhangye City, Gansu Province, comprising the following steps:

[0033] Step 1, cleanness processing: screen with a 5mm sieve to remove impurities and other plant seeds to obtain the clean seeds of X. sorbifolium;

[0034] Step 2, get test sample: from the net seed of step 1 clarity process, get 500 test samples at random, be divided into 4 repetitions, each repeats 125 seeds;

[0035] Step 3, sample pre-wetting: put the four repeated test samples taken in step 2 into a beaker, and soak in water at 15°C-25°C for 12 hours;

[0036] Step 4, mechanically remove the exocarp: compress the pre-wetted seed sample in step 3 with forceps, mechanically remove the exocarp, leave the endocarp and embryo, and do not damage the embryo. ...

Embodiment 2

[0042] In the present embodiment, a kind of in vitro embryo culture method with in-band testa of rare woody economic crop sorbifolium sorbifolium seed viability is measured, and the sample is from Inner Mongolia, and step 1 and step 2 are identical with embodiment 1;

[0043] Step 3, sample pre-wetting: put the four repeated test samples taken in step 2 into a beaker, and soak in water at 15°C-25°C for 8 hours;

[0044] Step 4, mechanically remove the exocarp: press the pre-wetted seed sample in step 3 with tweezers, mechanically remove the exocarp, leave the endocarp and embryo, and do not damage the embryo. For shriveled, discolored, moldy or empty seeds after taking the outer testa, treat them as dead seeds, and calculate the seed mortality;

[0045] Step 5, culture sand preparation: pass the river sand through a 0.05-0.8mm sieve, wash it with clean water, and then sterilize it at 140-145°C for 2 hours, and mix the sterilized sand and distilled water according to the ratio ...

Embodiment 3

[0050] A method for culturing in vitro embryos with testa in a rare woody economic crop sorbifolium sorbifolium seed viability measurement, the seed batch is from the Northwest sorbifolium sorbifolium base in Jingtai County, Gansu Province, and step 1 and step 2 are the same as in Example 1;

[0051] Step 3, sample pre-wetting: put the four repeated test samples taken in step 2 into a beaker, and soak in water at 15°C-25°C for 10 hours;

[0052] Step 4, mechanically remove the exocarp: press the pre-wetted seed sample in step 3 with tweezers, mechanically remove the exocarp, leave the endocarp and embryo, and do not damage the embryo. For shriveled, discolored, moldy or empty seeds after taking the outer testa, treat them as dead seeds, and calculate the seed mortality;

[0053] Step 5, preparation of the culture paper bed: soak the special germination paper (Anchor paper in the United States) with distilled water in the germination tray for 2-3 hours to saturate the germinati...

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Abstract

The invention discloses an endopleura-containing in-vitro embryo culture method for determining vitality of seeds of xanthoceras sorbifolia. The endopleura-containing in-vitro embryo culture method comprises the following continuous procedures, methods and steps of (1) cleanliness treatment of to-be-detected samples; (2) separation of test samples; (3) pre-wetting of seeds; (4) removing of outer peels by machines; (5) preparation of a culture bed; (6) culture of in-vitro embryos; (7) verification of vitality. In the procedures, methods and steps, during pre-wetting of seeds, seed samples are soaked into water with a temperature of 15 to 25 DEG C for 8 to 12 h, and pressed by tongs and tweezers; seed peel is removed by the machines, a complete seed embryo reserved within the endopleura is cultured in a wet paper bed or a sand bed for 3 to 5 days under the light radiation conditions of 15 DEG C, 20 DEG C, 25 DEG C, or 15 DEG C and 25 DEG C alternately; finally, the vitality is verified.The endopleura-containing in-vitro embryo culture method has the advantages that the standards of dead seeds and vital seeds are described; the principle is reliable, the method is simple and convenient, the cost is low, and the redemonstration is quick; the endopleura-containing in-vitro embryo culture method is suitable for determining the vitality and dormant states of seed batches of differentsources and seed batches of different dormant states.

Description

technical field [0001] The invention relates to the technical field of cultivating woody economic crops, in particular to a method for culturing isolated embryos with testa in vitro for measuring the viability of Xanthos sorbifolium seeds. Background technique [0002] Xanthoceras sorbifolium Bunge is a perennial deciduous shrub or small tree of the genus Xanthoceras Bunge in the family Sapindaceae. It is native to the Loess Plateau in northern my country and is naturally distributed in northern latitudes 32°~46°, east longitude 100°~127°, that is, to the west of Liaoning and southwest of Jilin in the north, from Xiaoxian County of Anhui Province and southern Henan in the south, to Shandong in the east, and to Gansu and Ningxia in the west. It is concentrated in Inner Mongolia, Shaanxi, Shanxi, Hebei, Gansu and other places, and a small amount is distributed in Liaoning, Jilin, Henan, Shandong and other provinces. [0003] Xantho sorbifolium is resistant to drought, barrenne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王进颜霞刘贤德戴斐斌李军元谢全刚严谈松
Owner HEXI UNIV
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