Instant culture device for detecting flora by most probable number method

Abstract

The invention relates to the field of flora detection, in particular to an instant culture device for detecting flora by a most probable number method. In the prior art, a reverse Durham tube is usedas a culture tube of an MPN method; the fermentation condition is judged by observing the gas generation in a small reverse tube, but the space of the culture tube is narrow and small; before use, theair in the small reverse tube is difficult to be completely eliminated. In order to solve the technical problems, the invention provides an instant fermentation culture tube for MPN. The culture tubecomprises a hard main tube and a hard gas generating tube which are connected through a bent connector; the sealed hard gas generating tube is used for replacing the built-in small reverse tube in the prior art and is not put into the fermentation tube, so that the limitation by the built-in tube diameter and the limitation by the reverse position are avoided; therefore the inner diameter of thehard gas generating collecting tube and the reverse angle selection freedom are increased; the tube line connection of the hard main tube and the hard gas generating collecting tube is more smooth; the gas exhaust is convenient; the advantages of high speed, high efficiency, time saving, labor saving and the like are realized.

Description

technical field

[0001] The invention relates to the field of flora detection devices, in particular to a ready-to-use culture device and a use method for detection of coliforms, heat-resistant coliforms and Escherichia coli most probable number (MPN). Background technique

[0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art.

[0003] The detection of coliforms, heat-resistant coliforms (fecal coliforms) and Escherichia coli in the hygienic microbiological indicators of public products such as water, food, cosmetics and public places includes the multi-tube fermentation most probable number (MPN) method, Membrane filtration method, plate counting method, enzyme substrate method, etc. Among them, the multi-t...

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