Instant culture device for detecting flora by most probable number method

Abstract

The invention relates to the field of flora detection, in particular to an instant culture device for detecting flora by a most probable number method. In the prior art, a reverse Durham tube is usedas a culture tube of an MPN method; the fermentation condition is judged by observing the gas generation in a small reverse tube, but the space of the culture tube is narrow and small; before use, theair in the small reverse tube is difficult to be completely eliminated. In order to solve the technical problems, the invention provides an instant fermentation culture tube for MPN. The culture tubecomprises a hard main tube and a hard gas generating tube which are connected through a bent connector; the sealed hard gas generating tube is used for replacing the built-in small reverse tube in the prior art and is not put into the fermentation tube, so that the limitation by the built-in tube diameter and the limitation by the reverse position are avoided; therefore the inner diameter of thehard gas generating collecting tube and the reverse angle selection freedom are increased; the tube line connection of the hard main tube and the hard gas generating collecting tube is more smooth; the gas exhaust is convenient; the advantages of high speed, high efficiency, time saving, labor saving and the like are realized.

Description

technical field [0001] The invention relates to the field of flora detection devices, in particular to a ready-to-use culture device and a use method for detection of coliforms, heat-resistant coliforms and Escherichia coli most probable number (MPN). Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The detection of coliforms, heat-resistant coliforms (fecal coliforms) and Escherichia coli in the hygienic microbiological indicators of public products such as water, food, cosmetics and public places includes the multi-tube fermentation most probable number (MPN) method, Membrane filtration method, plate counting method, enzyme substrate method, etc. Among them, the multi-t...

AI Technical Summary

Problems solved by technology

During the transportation of the above-mentioned ready-to-use fermentation tube containing liquid medium, the small inverted tube is easy to be filled with gas again under the action of external force, and the small inverted tube is also easy to be filled when the sample solution is added to the ready-to-use fermentation tube containing dry powder medium. Before using each fermentation tube, the experimenter needs to carefully invert the small inverted tube several times to discharge the gas. The small inverted tube has a small diameter and is inverted at an angle of 180 to the fermentation test tube. It is difficult to discharge the gas and requires skilled experimenters. Choose a suitable angle, fill the liquid slowly, and press out the gas. Each tube needs to be operated carefully, and it will take a long time

However, coliforms, heat-resistant coliforms (fecal coliforms) and Escherichia coli belong to the hygienic microbial indicators of public products such as water, food, cosmetics and public places. A sample requires 1-30 fermentation tubes, so no matter whether the experimenter prepares the medium himself or uses the ready-to-use fermentation tube provided by the medium manufacturer to discharge small inverted tube gas, it will consume a lot of human resources and time resources, and For the liquid type ready-to-use fermentation tube, the storage requirements are relatively high, and the shelf life is short, which increases the cost of use; once the dry powder-type ready-to-use fermentation tube is filled with more dry powder in the small inverted tube, it does not use the medium to mix in the sample liquid. When mixing, it is easy to generate fine bubbles in the small inverted tube, which increases the difficulty of exhausting, reduces the quality of detection and increases the cost of detection.

Therefore, the small inverted tube exhaust problem is a bottleneck technical limitation that affects the efficiency of the multi-tube fermentation most probable number (MPN) method, manpower and time costs, and the convenience of ready-to-use media.

The inventor found that based on the above-mentioned problems, it is difficult for the manufacturer to provide a ready-to-use culture tube. Due to the large number of cultures required during the experiment, researchers have to spend a lot of time on the preparation of the culture tube , providing a low-cost, ready-to-use culture tube can greatly save the time and manpower required for detection

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