Microbial cell factory based on photoinduced dielectrophoresis technology and application thereof

A light-induced dielectrophoresis and microbial cell technology, applied in the field of fermentation, can solve problems such as complicated procedures, long parameter selection time, and difficulty in obtaining optimal parameters, so as to improve fermentation efficiency, shorten optimization cycle, and uniform excellent strains Effect
CN109679845AActive Publication Date: 2019-04-26JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2019-04-26

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Abstract

The invention discloses a microbial cell factory based on a photoinduced dielectrophoresis technology and application thereof, and belongs to the technical field of fermentation. The microbial cell factory based on the photoinduced dielectrophoresis technology comprises an ITO (Indium Tin Oxide) sandwich mechanism, a cell factory mechanism, an observation mechanism and an optical operation mechanism, wherein the cell factory mechanism is arranged inside the ITO sandwich mechanism; the cell factory mechanism includes a cell warehouse, a virtual conveyor belt, a cell culture plate and a cell plate frame. The cell factory can be constructed at the micron scale level, and accurate and rapid screening of excellent strains and extraction of the optimal parameters of a fermentation process can berealized simultaneously in the same factory. Compared with the traditional fermentation process, the method greatly shortens the cycle of strain screening and fermentation parameter optimization, andthe excellent strains obtained from the cell level are more uniform and accurate. The optimized parameters of fermentation is closer to the essential characteristics of cells and are optimal, therebyimproving the fermentation efficiency and promoting the development of the microbial industry.
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Description

technical field

[0001] The invention relates to a microbial cell factory constructed based on light-induced dielectrophoresis technology and an application thereof, belonging to the technical field of fermentation. Background technique

[0002] Biological fermentation engineering is an important part of biotechnology and an important link in the industrialization of biotechnology. It plays an increasingly important role in high value-added industries such as modern food and medicine. From a microscopic perspective, the essence of microbial fermentation engineering is the repeated physiological metabolism and production and reproduction process of the fermenting bacteria in the colony starting from spore inoculation and shedding the next spore. The products of the metabolism or reproduction process are the output of the fermentation industry. The entire fermentation process can be roughly divided into two parts: strain selection and colony fermentation.

[0003] Breeding of ...

Claims

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