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Microbial cell factory based on photoinduced dielectrophoresis technology and application thereof

A light-induced dielectrophoresis and microbial cell technology, applied in the field of fermentation, can solve problems such as complicated procedures, long parameter selection time, and difficulty in obtaining optimal parameters, so as to improve fermentation efficiency, shorten optimization cycle, and uniform excellent strains Effect

Active Publication Date: 2019-04-26
JIANGNAN UNIV
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Problems solved by technology

[0006] The present invention aims at the deficiencies such as long period, high cost and complicated process in the fermentation strain breeding process in the current microbial industry, the difficulty in obtaining optimal parameters in the colony fermentation process, long parameter selection time and other shortcomings, as well as strain selection and colony fermentation. To solve problems such as the disconnection of the two processes, provide a method and equipment for constructing microbial cell factories and realizing precise fermentation based on light-induced dielectrophoresis technology

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  • Microbial cell factory based on photoinduced dielectrophoresis technology and application thereof
  • Microbial cell factory based on photoinduced dielectrophoresis technology and application thereof
  • Microbial cell factory based on photoinduced dielectrophoresis technology and application thereof

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Embodiment 1

[0031] The principle of equipment for constructing microbial cell factories based on light-induced dielectrophoresis technology is as follows: figure 1 shown. The equipment mainly includes four parts: observation part, ITO (indium tin oxide) sandwich mechanism part, microbial cell factory part and light operation part.

[0032]The observation part is mainly composed of a high-definition CCD and related software, which is used to observe or record the process of cell factory establishment, microbiological cell screening, and cell plate rack operation. The ITO sandwich mechanism mainly consists of two layers of ITO glass and an intermediate layer. The upper surface of the lower ITO glass is coated with a layer of hydrogenated amorphous silicon with a thickness of about 0.5-1 μm. The intermediate layer is fixed by a fixing glue. Spaces such as cell factories and cell operations are isolated. ITO glass is coated with a layer of ITO film with a thickness of about 180nm on the gla...

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Abstract

The invention discloses a microbial cell factory based on a photoinduced dielectrophoresis technology and application thereof, and belongs to the technical field of fermentation. The microbial cell factory based on the photoinduced dielectrophoresis technology comprises an ITO (Indium Tin Oxide) sandwich mechanism, a cell factory mechanism, an observation mechanism and an optical operation mechanism, wherein the cell factory mechanism is arranged inside the ITO sandwich mechanism; the cell factory mechanism includes a cell warehouse, a virtual conveyor belt, a cell culture plate and a cell plate frame. The cell factory can be constructed at the micron scale level, and accurate and rapid screening of excellent strains and extraction of the optimal parameters of a fermentation process can berealized simultaneously in the same factory. Compared with the traditional fermentation process, the method greatly shortens the cycle of strain screening and fermentation parameter optimization, andthe excellent strains obtained from the cell level are more uniform and accurate. The optimized parameters of fermentation is closer to the essential characteristics of cells and are optimal, therebyimproving the fermentation efficiency and promoting the development of the microbial industry.

Description

technical field [0001] The invention relates to a microbial cell factory constructed based on light-induced dielectrophoresis technology and an application thereof, belonging to the technical field of fermentation. Background technique [0002] Biological fermentation engineering is an important part of biotechnology and an important link in the industrialization of biotechnology. It plays an increasingly important role in high value-added industries such as modern food and medicine. From a microscopic perspective, the essence of microbial fermentation engineering is the repeated physiological metabolism and production and reproduction process of the fermenting bacteria in the colony starting from spore inoculation and shedding the next spore. The products of the metabolism or reproduction process are the output of the fermentation industry. The entire fermentation process can be roughly divided into two parts: strain selection and colony fermentation. [0003] Breeding of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/36C12M1/00
CPCC12M23/04C12M23/20C12M23/48C12M35/02C12M41/48C12M47/02
Inventor 李恭新刘飞陈珺
Owner JIANGNAN UNIV
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