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A kit and detection method for detecting foot-and-mouth disease virus O-type protective antibody

A foot-and-mouth disease virus and antibody detection technology is applied in the field of immunology to achieve the effects of simple operation, reliable and accurate detection results, and simple and convenient operation

Active Publication Date: 2020-11-24
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, at present, there is no detection method for the detection of FMD virus O-type protective antibody that is highly relevant, easy to operate, sensitive and stable.

Method used

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  • A kit and detection method for detecting foot-and-mouth disease virus O-type protective antibody
  • A kit and detection method for detecting foot-and-mouth disease virus O-type protective antibody
  • A kit and detection method for detecting foot-and-mouth disease virus O-type protective antibody

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1 Materials and methods

[0043] 1.1 Materials

[0044] Carbonate buffer (Na 2 CO 3 / NaHCO 3), BSA were purchased from SIGMA company. Foot-and-mouth disease virus type O bovine broad-spectrum neutralizing antibody capture antibody and detection antibody are prepared and stored in this laboratory (for the preparation method, please refer to the patent: A method for preparing a broad-spectrum neutralizing antibody of whole bovine source O-type foot-and-mouth disease virus, application number: 201810929067.1, Wherein the capture antibody is the whole bovine monoclonal antibody 11 recorded in the patent document, the detection antibody is the whole bovine monoclonal antibody 14), the capture antibody and the detection antibody pair FMDV O / Mya / 98 (SEA), FMDV O / ZK / 93 ( Cathay) and FMDV O / HK / 99 (ME-SA) had neutralization titers >128; LPBE titers <4. FMDV O-type 146S antigen (O / Mya / 98 strain) was prepared and preserved in our laboratory.

[0045] 1.2 Serum samples

[004...

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Abstract

The invention provides a kit and detection method for detecting a type O protective antibody of the foot and mouth disease virus, and belongs to the technical field of immunology. The kit is preparedfrom a capturing antibody, a detecting antibody, biotin, an FMDVO type 146 S antigen, Strep-HRP, a TMB substrate, an encapsulating buffer and blocking fluid; the capturing antibody and the detecting antibody are type O bovine broad-spectrum neutralizing antibodies of the foot and mouth disease virus. The detection kit and method combine the advantages of two methods of virus neutralization tests and liquid phase blocking ELISA, the detection method is simple in operation, and the detection result is reliable and accurate.

Description

technical field [0001] The invention belongs to the technical field of immunology, and in particular relates to a kit and a detection method for detecting the O-type protective antibody of foot-and-mouth disease virus. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, febrile and highly contagious infectious disease of artiodactyls caused by foot-and-mouth disease virus (FMDV). It is known that once the disease breaks out, it will cause huge economic losses and negative social and political impacts. The International Organization for Animal Health (OIE) lists the disease as the top of the legally reported animal infectious diseases, and the Chinese government also ranks it in the first category of animal infectious diseases. The first place of the disease (Xie Qingge. Foot-and-mouth disease. Beijing: China Agricultural Press, 2004:1-15.). There are seven serotypes of FMDV, namely O, A, C, Asia 1, SAT1, SAT2, and SAT3. Among them, FMDV O-type exists wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/577
CPCG01N33/56983G01N33/577G01N33/6854G01N2333/09
Inventor 曹轶梅李坤卢曾军刘在新王省付元芳周莎莎包惠芳孙普李平花白兴文张婧马雪青李冬
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI