Feed additive for strengthening immunity of tilapia
A technology of feed additive and immunity, which is applied in the field of feed additive to enhance the immunity of tilapia, and can solve the problems of loss of liver function, lack of disease resistance, and difficulty in finding treatment measures for sick fish
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Embodiment 1
[0021] The preparation method of the enzyme extract of fish scale and horsefly powder is:
[0022] 1) Mix fish scales and horsefly powder to obtain a mixture, then add water for beating to obtain a slurry; the mass ratio of fish scales to horsefly powder is 3:1.
[0023] 2) After the temperature of the slurry is raised to 50° C., the pH is adjusted to 7.2, and then the compound enzyme I of papain and neutral protease is added, and the enzymatic hydrolysis reaction is carried out for 8 hours; the amount of the compound enzyme I added is 4% of the weight of the mixture;
[0024] 3) After cooling the enzymolysis solution in step 2) to 40°C, adjust the pH to 5.2, then add the complex enzyme II of acid protease and nuclease, carry out the enzymolysis reaction for 6 hours, and then centrifuge to obtain the supernatant, supernatant It can be concentrated and dried. The compound enzyme II is added in an amount of 10% by weight of the mixture.
[0025] The pretreatment method of the ...
Embodiment 2
[0028] The preparation method of the enzyme extract of fish scale and horsefly powder is:
[0029] 1) Mix fish scales and horsefly powder to obtain a mixture, then add water for beating to obtain a slurry; the mass ratio of fish scales to horsefly powder is 5:1.
[0030] 2) After the temperature of the slurry is raised to 60° C., the pH is adjusted to 6.5, and then compound enzyme I of papain and neutral protease is added to carry out enzymatic hydrolysis reaction for 12 hours; the amount of compound enzyme I added is 6% of the weight of the mixture;
[0031] 3) After cooling the enzymolysis solution in step 2) to 46°C, adjust the pH to 4.5, then add the complex enzyme II of acid protease and nuclease, carry out the enzymolysis reaction for 10 hours, and then centrifuge to obtain the supernatant, the supernatant It can be concentrated and dried. The amount of compound enzyme II added is 5% of the weight of the mixture.
[0032] The pretreatment method of the pretreated fly a...
Embodiment 3
[0035] A feed additive for enhancing the immunity of tilapia, calculated by weight percentage, comprising the following raw materials:
[0036] 30 parts of enzyme extracts of fish scale and horsefly powder of embodiment 1, 6 parts of poria cocos, 10 parts of yam, 2 parts of pomegranate peel, 10 parts of chili oil and 12 parts of pretreated fly ash of embodiment 2.
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