Multifunctional nano-drug delivery carrier targeting lung cancer genes and its preparation and application

A technology of nano-drugs and delivery carriers, which is applied in drug combinations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc. It can solve the problems of no photothermal effect and poor targeting, and achieve improved stability. And the effects of biocompatibility, uniform shape, and improved loading efficiency

Active Publication Date: 2020-09-29
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional nano-drug carrier delivery systems have poor targeting and no photothermal effect

Method used

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  • Multifunctional nano-drug delivery carrier targeting lung cancer genes and its preparation and application
  • Multifunctional nano-drug delivery carrier targeting lung cancer genes and its preparation and application
  • Multifunctional nano-drug delivery carrier targeting lung cancer genes and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] This example provides a method for preparing a multifunctional nano-drug delivery carrier targeting lung cancer genes, which consists of the following steps:

[0051] (1) Synthesis of nano-gold triangle sheet GNPs

[0052] Take 1.6ml CTAC (Cetyltrimethylammonium Chloride, 100mM) mixed with 8mL deionized water, then add 80μL HAuCl 4 (25.4mM), 75μL KI (10mM), 20.3μL NaOH (0.1M), quickly add 80μL of AA (ascorbic acid, 64mM), mix slightly, the color of the solution becomes colorless, then quickly add 20μL of NaOH (0.1M) To terminate the reduction reaction, the reaction bottle should be placed in an oven at 28 degrees Celsius for 10 minutes. Then carry out centrifugation, the centrifugation speed is 11000rpm, 30min, and the precipitate obtained is nano-gold triangle sheet GNPs, and the average diameter is 42±2.8nm.

[0053] (2) Nano-gold triangular sheet surface connection complex PSS

[0054] Prepare 20ml GNPs solution according to step (1), add 4ml PSS solution (10mg mL...

Embodiment 2

[0061] This example provides a method for preparing a multifunctional nano-drug delivery carrier targeting lung cancer genes, which consists of the following steps:

[0062] (1) Synthesis of nano-gold triangle sheets

[0063] First, mix 8mL CTAC (100mM) with 40mL deionized water, then add 400μL HAuCl4 (25.4mM), 375μL KI or NaI (10mM), 115μL NaOH (0.1M), and mix well. At this time, the color of the solution is light yellow ; Then, quickly add 400 μL of AA (96mM), mix slightly, the color of the solution becomes colorless, then quickly add 50 μL of NaOH (0.1M) to terminate the reduction reaction, and place the reaction bottle in an oven at 29 degrees Celsius for 15 minutes . Then centrifuge at a speed of 10000 rpm for 20 minutes, and the precipitate obtained is the nano-gold triangle sheet GNPs with an average diameter of 89±5.8nm.

[0064] (2) Nano-gold triangular sheet surface connection complex PSS

[0065] Prepare 20ml GNPs solution according to step (1), add 4ml PSS solut...

Embodiment 3

[0071] This example provides a method for preparing a multifunctional nano-drug delivery carrier targeting lung cancer genes, which consists of the following steps:

[0072] (1) Synthesis of nano-gold triangle sheets

[0073] First, mix 1.6ml CTAC (100mM) with 8mL deionized water, then add 80μL HAuCl 4 (25.4mM), 100μL of KI (10mM), 20.3μL of NaOH (0.1M), quickly add 80μL of AA (128mM), mix slightly, the color of the solution becomes colorless, then quickly add 10μL of NaOH (0.1M) to stop the reduction For the reaction, the reaction bottle should be placed in an oven at 28 degrees Celsius for 10 minutes. Then carry out centrifugation, the centrifugation speed is 10000rpm, 30min, and the precipitate obtained is nano-golden triangle sheet GNPs, with an average diameter of 115±7.8nm.

[0074] (2) GNPs surface junction complex PSS

[0075] Prepare 40mL GNPs solution according to step (1), add 8mL PSS solution (10mg mL -1 , using 1mM NaCl solution to prepare) solution and 4mL Na...

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Abstract

The present invention provides a multifunctional nano drug delivery carrier targeting lung cancer genes and a preparation and an application thereof. The nano drug delivery carrier comprises a nano gold triangle piece, a surface of the nano gold triangle piece is sequentially connected with a complex poly(sodium 4-styrene sulfonate) and a complex poly(diallyldimethylammonium chloride) to form a nano gold triangle piece complex; and a siRNA nano drug targeting the lung cancer genes adsorbs and is linked to the nano gold triangle piece complex. The siRNA nano drug is connected with GNPs / PSS / PDADMAC, thereby obtaining the multifunctional nano drug delivery carrier having both targeting and photothermal therapy and immunotherapy.

Description

technical field [0001] The present invention relates to the fields of biomedicine, photothermal therapy, immunotherapy and tumor therapy, in particular to the preparation and application of a nano drug delivery carrier targeting lung cancer genes. Background technique [0002] The currently recognized cancer treatment methods around the world usually include surgery, chemotherapy, radiotherapy and immunotherapy. However, due to the great diversity of tumors, these methods still face severe challenges, because they are not effective enough to target the cancer site and cannot completely eliminate the cancer. cells, especially in advanced cancer patients. Therefore, more and more studies focus on improving the targeting of tumors and performing multiple overlapping treatments. Photothermal therapy (PTT) has great promise for the treatment of various cancers. This therapy uses near-infrared laser to irradiate the tumor site, which can cause high temperature in the tumor site, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K31/7105A61K47/32A61P35/00
Inventor 崔大祥刘彬
Owner SHANGHAI JIAOTONG UNIV
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