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Methods for suppression of ammonia formation from staphylococcus-xylosus and staphylococcus-cohnii

An odor suppressant, bacterial technology, used in poultry, cats and horses, can solve problems such as greenhouse effect

Inactive Publication Date: 2019-05-03
DOW GLOBAL TECH LLC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, emissions from animal litter can contribute to the greenhouse effect

Method used

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  • Methods for suppression of ammonia formation from staphylococcus-xylosus and staphylococcus-cohnii
  • Methods for suppression of ammonia formation from staphylococcus-xylosus and staphylococcus-cohnii
  • Methods for suppression of ammonia formation from staphylococcus-xylosus and staphylococcus-cohnii

Examples

Experimental program
Comparison scheme
Effect test

example 1、2、3 and 4

[0040] Examples 1, 2, 3 and 4. Lab experiments:

[0041] Synthetic poultry urine preparation. In these examples, for each 100 g of synthetic poultry urine required, synthetic urine was prepared as follows. The amount can be scaled up or down as needed. Add the following components to the sterile bottle: Urea (USP grade): 0.43g, Ammonia: 0.46g, K 3 PO 4 (USP grade): 6.8g, Milli-Q water: 83.52g. The bottles were capped and placed on a bottle tumbler at ambient temperature for 1 hour to 3 hours. Measure the pH of the solution to confirm that the pH is between 13 and 14. To add 7.5gr of synthetic urine to the samples (Examples 1 and 2), 0.66g USP grade uric acid and 6.84mL of the prepared solution were added to a 50mL centrifuge tube and vortexed for 15 seconds on the high setting. To add 10 gr of synthetic urine to the samples (Examples 3 and 4), 0.88 g of USP grade uric acid and 9.12 mL of the prepared solution were added to a 50 mL centrifuge tube and vortexed for 15 sec...

example 1 and 2

[0048] For Examples 1 and 2, 7.5 g of synthetic urine prepared as detailed herein was mixed with 2.28 mL of MilliQ water and 1 mL of sterile phosphate-buffered saline solution for 10 seconds using a vortex mixer on the high setting. 1 mL of overnight bacterial growth solution prepared as detailed previously was added to the mixture and vortexed for 10 sec on the high setting.

example 3 and 4

[0049] For Examples 3 and 4, 2 mL of overnight bacterial growth solution prepared as detailed herein was added to 10 gr of synthetic urine prepared as previously described and vortexed for 10 seconds on the high setting.

[0050] Immediately after preparation, a mixture of synthetic urine and bacteria was added to the samples and mixed with the straw using a sterile inoculating loop. The samples were then covered with aluminum foil to prevent vapors from escaping from the container. Ammonia levels in the headspace were measured on days 1 and 4 after inoculation.

[0051] Add processing:

[0052] After the day 4 measurement, the aluminum foil on the samples was removed and the desired treatments were added. Open the aluminum foil for the same amount of time as the untreated control sample.

[0053] The treatments of several examples are detailed below; Table 1 details which treatment was applied to each specific example.

[0054] Processing of instances 1 and 2:

[0055]...

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PUM

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Abstract

A method for suppressing the formation of ammonia comprising providing a carrier material to a container having a headspace; providing a bacteria, an acidifier and an odor inhibitor to the carrier material, the bacteria comprising Staphylococcus-xylosus or Staphylococcus-cohnii bacteria, and the odor inhibitor comprising a salt of an aminopolycarboxylic acid compound; and applying animal waste tothe carrier material; wherein there is a 5 to 98 percent improvement of ammonia content in the headspace as compared to an untreated control comprising a container containing the carrier material andthe bacteria and not contain the odor inhibitor.

Description

Background technique [0001] Animal litter is used as a trapping material for feces and urine. These biological by-products can produce strong odors due to the evolution of malodorous compounds, especially ammonia. Ammonia is produced by microbial action on urea and / or uric acid in animal manure. [0002] The smell of animal litter is distressing and can be toxic at high enough levels. Additionally, odors can be a nuisance for animal well-being, especially in closed environments such as poultry houses and stables / barns. In addition, emissions from animal litter can contribute to the greenhouse effect. Accordingly, it would be desirable to find ways to control odor in animal litter. [0003] The problem addressed by the present invention is a method for inhibiting the formation of ammonia by odor producing bacteria such as Staphylococcus xylosus and Staphylococcus couriformis. Contents of the invention [0004] A method for inhibiting the formation of ammonia, comprising:...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K1/015
CPCA01K1/0152A61L9/014B01J2220/4868B01J20/02B01J20/22
Inventor L·黑文斯M·比尔曼J·A·博娜蒂斯B·D·霍克A·伊兹米特里K·A·索伯
Owner DOW GLOBAL TECH LLC
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