Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Engineered glucosyltransferases

A technology of glucosyltransferase and glucan, which is applied in the field of enzyme catalysis and can solve problems such as improvement in yield of glucan that rarely pays attention to

Pending Publication Date: 2019-05-03
NUTRITION & BIOSCIENCE USA 4 INC
View PDF20 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] While these and other advances have been made in the production of glucan polymers using glucosyltransferases, little attention appears to have been paid to the improvement of glucan yields from such enzymes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Engineered glucosyltransferases
  • Engineered glucosyltransferases
  • Engineered glucosyltransferases

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0137] [alpha]-glucan produced in the glucan synthesis methods herein may optionally be isolated. In certain embodiments, isolating the alpha-glucan product comprises at least the steps of centrifugation, filtration, fractionation, chromatography, dialysis, evaporation, or dilution. By way of example only, insoluble α-glucan can be separated by centrifugation or filtration, whereas soluble α-glucan can be separated by chromatography or dialysis. Isolation may optionally further comprise washing the alpha-glucan product with water or other aqueous solution one, two or more times, and / or drying the alpha-glucan product.

[0138]Any of the disclosed conditions for the synthesis of α-glucans (such as those described above or in the examples below) can be applied to the practice of the presently disclosed reaction compositions (and vice versa), and / or thus used in Characterization of the characteristics / activity of non-native glucosyltransferases.

[0139] The present disclosure ...

example

[0176] The disclosure is further illustrated in the following examples. It should be understood that these Examples, while illustrating certain preferred aspects herein, are given by way of illustration only. From the above discussion and these Examples, one skilled in the art can ascertain the essential characteristics of the disclosed embodiments, and without departing from the spirit and scope thereof, can make various changes and modifications to make the disclosed embodiments Adapt to many uses and conditions.

example 1

[0178] Analysis of amino acid sites affecting the selectivity of glucosyltransferases for α-glucan synthesis

[0179]This example describes the screening of glucosyltransferase variants with improved selectivity for alpha-glucan synthesis from sucrose. Another objective of this screen was to identify glucosyltransferase variants that exhibit reduced synthesis of by-products such as leuconostoc and glucose-oligosaccharides. Variants with either or both of these yield properties were identified.

[0180] The amino acid sequence of the glucosyltransferase used to make the amino acid substitutions in this example is SEQ ID NO: 4 (GTF 6855), which is essentially the full-length wild-type glucosyltransferase from S. salivarius SK126 (see Table 1). N-terminal truncated (signal peptide and variable region removed) form of glycosyltransferase (represented by SEQ ID NO: 62). Substitutions made in SEQ ID NO: 4 can be characterized as substituting natural amino acid residues, because ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and / or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.

Description

[0001] This application claims the benefit of US Provisional Application No. 62 / 394,354 (filed September 14, 2016), which is hereby incorporated by reference in its entirety. technical field [0002] This disclosure is in the field of enzyme catalysis. For example, the present disclosure relates to glucosyltransferases with modified amino acid sequences. Such modified enzymes have improved product yield properties. [0003] References to Sequence Listings Submitted Electronically [0004] The official copy of this Sequence Listing is submitted electronically via EFS-Web as a Sequence Listing in ASCII format with the file name CL6395USNP_SequenceListing_ST25_ExtraLinesRemoved.txt, created on September 11, 2017, and has a size of 612 kilobytes, and is at the same time as this specification submit. The Sequence Listing contained in this file in ASCII format is part of this specification and is hereby incorporated by reference in its entirety. Background technique [0005] ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/18C12P19/04C08B37/00
CPCC08B37/0009C12N9/1051C12P19/04C12P19/18
Inventor 李友根S.M.轩尼诗S.克拉尔M.S.佩恩V.阿尔坎程琼R.迪克西莫E.D.森科J.B.帕克R.R.波特
Owner NUTRITION & BIOSCIENCE USA 4 INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com