Preparation method of corn straw feed leavening agent
The technology of corn stalk and starter is applied in the field of preparation of corn stalk feed starter, which can solve the problems of poor palatability and limited use range, and achieve the effects of simple operation, wide use range and wide applicability.
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[0029] Weigh 10g of casein peptone, 5g of beef extract, 4g of yeast powder, 20g of glucose, 2g of triammonium citrate, 5g of sodium acetate, 1g of Tween 80, K 2 HPO 4 2g, MgSO 4 .7H 2 O 0.2g, MnSO 4 .H 2 O 0.05g, add water to dilute to 1L, adjust the pH to 6.8, insert Lactobacillus plantarum from the slope, and culture it statically at 37°C for 19 hours to obtain the Lactobacillus plantarum culture solution. Centrifuge the Lactobacillus plantarum culture solution at 4000 rpm for 20 minutes to obtain the Lactobacillus plantarum sludge.
[0030] Weigh 50 g of soybean protein peptide, 30 g of glucose, 30 g of yeast extract, K 2 HPO 4 1g, MgSO 4 .7H 2 O 0.5g, add water to dilute to 1L, adjust the pH to 6.5, insert Leuconostoc enteritidis from the slope, and culture it statically at 30°C for 19 hours to obtain the Leuconostoc enteritidis culture solution. The Leuconostoc enterolis culture solution was centrifuged at 4000 rpm for 20 minutes to obtain the Leuconostoc enter...
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