17 results about "Leuconostoc sp." patented technology

The invention discloses a method for synthesizing alpha-arbutin through biotransformation, and belongs to the technical field of biochemistry. A gene cloning technology is utilized, sucrose phosphorylase Spase in leuconostoc mesenteroides and sucrose phosphorylase gtfa in bifidobacterium adolescentis are screened and found through a Deep Molecules and DLKcat algorithm on a Uniprot database, novopro is utilized for codon optimization, then the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa are expressed on a plasmid vector PET-28a and are converted into an escherichia coli BL21 (DE3) strain, and the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa in the bifidobacterium adolescentis are obtained. An engineering strain for heterologous expression of the gene is constructed by taking the gene as a chassis cell. According to the process, sucrose and hydroquinone are taken as co-substrates, and bioconversion is carried out under the action of sucrose phosphorylase from different sources to obtain alpha-arbutin.

The invention relates to a preparation method of a leavening agent, in particular to a preparation method and application of a lactic acid bacteria leavening agent suitable for fermenting sweet potatoes. The preparation method of the lactic acid bacteria starter comprises the following steps: respectively and continuously activating lactobacillus plantarum, lactobacillus buchneri and leuconostoc mesenteroides for 2-3 times, compounding an activating solution according to the mass ratio of 1: 1: 1, and fermenting sweet potatoes at the most suitable fermentation temperature of 23.5 DEG C for 3 days with the inoculum size of the starter being 3.5%, thereby ensuring that the strains efficiently ferment the sweet potatoes in the most suitable environment. The preparation method of the lactic acid bacteria starter is simple, low in cost and capable of achieving industrial production, opens up a new way for sweet potato processing and utilization, adds a new variety for the sweet potato product market and promotes value increase and efficiency increase of the sweet potato industry.

The invention relates to a strain of leuconostoc lactis, which is named as leuconostoc lactis Gill 3, and is preserved in Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO.65904, the preservation address is building 5, building 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation date is February 18, 2025. The leuconostoc lactis can generate antibacterial substances with narrow-spectrum activity, the antibacterial range is more accurate, the leuconostoc lactis has a remarkable antibacterial effect on common gram-positive bacteria and partial negative pathogenic bacteria in various foods, but the leuconostoc lactis has weak resistance to other indicator bacteria or has no antibacterial activity; meanwhile, the antibacterial substance not only has an inhibition effect on bacterial planktonic cells, but also can resist the formation of bacterial biofilms. Therefore, the leuconostoc lactis disclosed by the invention can be used for prolonging the shelf life of food, and even can be developed into a probiotic.

The present invention relates to a method for preparing a fermented guava leaf composition having antioxidant efficacy superior to that of conventional guava leaves by using lactic acid bacteria fermentation in a guava leaf extract. The present invention has an excellent effect of providing: the method for preparing a guava leaf composition fermented with lactic acid bacteria, in which lactic acid bacteria of Leuconostoc mesenteroides (MGE 3138), Lactobacillus plantarum (MGE 3143), and Leuconostoc citrium Cares-1 are inoculated into a guava leaf extract and fermented; and a food composition having improved antioxidant efficacy and palatability by using the composition.

The application discloses a Leuconostoc intestini and Lactococcus lactis composition and application, belongs to the field of microbial pharmacy, and relates to application of a Leuconostoc intestini and Lactococcus lactis composition to preparation of a drug for treating pulmonary fibrosis diseases; wherein the quantity of the Leuconostoc intestini and the Lactococcus lactis in the composition is equal in terms of colony forming units. Experimental results show that the Leuconostoc intestini and the Lactococcus lactis can effectively improve pulmonary fibrosis when being intervened alone or in combination. It is known to those skilled in the art that excessive deposition of collagen fibers is a core feature of pulmonary fibrosis diseases, and the Leuconostoc intestini and the Lactococcus lactis have a synergistic effect of inhibiting the content of lung tissue collagen in pulmonary fibrosis. Therefore, the Leuconostoc intestini and the Lactococcus lactis composition has a prospect of being developed into a drug for treating pulmonary fibrosis diseases (including idiopathic pulmonary fibrosis and secondary pulmonary fibrosis).

The present application relates to the technical field of microorganism, in particular to a leuconostoc lactis with good probiotic characteristics and application thereof in preparation of drugs for preventing and treating inflammatory bowel disease, and the leuconostoc lactis (Leuconostoc lactis) LL271 provided by the present application is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 25974.The strain has good acid tolerance, bile salt tolerance, self-aggregation ability, hydrophobicity and other probiotic characteristics such as the ability to inhibit common human pathogenic microorganisms; meanwhile, the strain can improve intestinal inflammation, has prevention and treatment effects on inflammatory bowel disease, has high biological safety, can be used as probiotic preparation for relieving inflammatory bowel disease, improving intestinal mucosal injury and colon inflammation, provides a new strategy for preventing and treating inflammatory bowel disease, and has a potential clinical application prospect.

The invention belongs to the field of micro-ecological feed additives, and discloses a leavening agent for whole corn silage. The leavening agent is composed of plant lactobacillus plantarum, lactobacillus brevis, leuconostoc mesenteroides and pediococcus acidilactici, strains of the plant lactobacillus plantarum, the lactobacillus brevis, the leuconostoc mesenteroides and the pediococcus acidilactici are fermented respectively, and then strain fermentation solutions obtained after fermentation are mixed according to the viable count ratio of (1-2): (1-2): (1-2): (1-2) to obtain the composite micro-ecological leavening agent. When the composite micro-ecological fermentation agent is used for whole-plant corn silage fermentation, the pH value can be rapidly reduced, the dry matter content can be increased, and the acid detergent fiber level can be reduced.

The application discloses a kind of ginseng fermentation mixed culture ferment and application thereof.Belongs to leisure food preparation technical field.The mixed culture ferment includes lactobacillus plantarum LNJ-HQ-01, Leuconostoc mesenteroides LNJ-L-014 and lactobacillus fermentum BNCC194390.The application also discloses a kind of preparation method of hawthorn ginseng leisure food, as follows: hawthorn is washed, cored;after ginseng powder is mixed with water, the mixed culture ferment of the application is used to ferment in dark and sealed;after hawthorn is treated, ginseng is mixed and microwave heating, beating, sieving, and fruit paste is made;fruit paste is mixed with syrup;after being pressed into sheet, microwave intermittent low-speed pulsating airflow drying is used.The beneficial effects obtained by the application are as follows:the mixed culture ferment in the application can effectively avoid bacterial contamination, improve the nutritional value of ginseng, improve the bitter taste of ginseng, and the hawthorn ginseng leisure food prepared by the mixed culture ferment has good nutritional value and palatability.

The invention discloses a probiotic composition, a compound fermented feed and application, and belongs to the technical field of livestock and poultry breeding. Comprising leuconostoc mesenteroides and lactococcus, the leuconostoc mesenteroides is named as a leuconostoc mesenteroides LM01 strain and is preserved in China General Microbiological Culture Collection Center on November 21, 2025, the preservation number is CGMCC: 36737, and the lactococcus is named as a lactococcus LC01 strain and is preserved in China General Microbiological Culture Collection Center on November 21, 2025. The strain is preserved in the China General Microbiological Culture Collection Center on November 21, 2025, and the preservation number is CGMCC: 36736. The compound fermented feed disclosed by the invention is fermented by specific compound probiotics, so that the physicochemical properties and the nutritional value of the feed are effectively improved, and the compound fermented feed shows the effects of increasing the laying rate, the daily egg weight and the feed intake in laying hen breeding, and has extremely high popularization and application values.

The invention discloses a composition of leuconostoc mesenteroides and lactococcus lactis and application thereof, belongs to the field of microbial pharmacy, and requests to protect application of the composition of leuconostoc mesenteroides and lactococcus lactis in preparation of drugs for treating pulmonary fibrosis diseases. Wherein in the composition, the number of the leuconostoc mesenteroides is equal to that of the lactococcus lactis in a colony forming unit. Experimental results show that pulmonary fibrosis can be effectively improved through independent or combined intervention of leuconostoc mesenteroides and lactococcus lactis. Technicians in the field know that excessive deposition of collagenous fibers is the core characteristic of pulmonary fibrosis lesion, and combined administration of leuconostoc mesenteroides and lactococcus lactis has the effect of synergistically inhibiting the collagen content of lung tissues in pulmonary fibrosis. Therefore, the composition of the leuconostoc mesenteroides and the lactococcus lactis has the prospect of being developed into medicines for treating pulmonary fibrosis diseases (including idiopathic pulmonary fibrosis and secondary pulmonary fibrosis).

The invention discloses a complex microbial inoculant containing bifidobacterium and application of the complex microbial inoculant. Comprising two or more of bifidobacterium lactis BL9, bifidobacterium longum subsp. Longum KS1, clostridium pratensis GZSY3214, Leuconostoc mesenteroides PM10, and Gluconacetobacter xylinus Q1, and further comprises a preparation method of the bifidobacterium lactis BL9, a preparation method of the bifidobacterium longum subsp. Longum KS1, a preparation method of the bifidobacterium longum subsp. Longum GZSY3214, a preparation method of the bifidobacterium longum, a preparation method of the bifidobacterium longum, a preparation method of the bifidobacterium longum and a preparation method of the bifidobacterium longum, and a preparation method of the bifidobacterium longum. The complex microbial inoculant can be used for preparing milk powder, weight-losing food and fruit and vegetable beverages, and is wide in application range.

A method for hydrolyzing an alpha-1,5 glucosyl-fructose bond in a levansucrose, e.g., a sucrose, is disclosed. The method comprises contacting the sucrose with an alpha-glucosidase, e.g., a transglucosidase or a glucoamylase, under suitable conditions, during which the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose bond of the sucrose. The method is used, e.g., to reduce the amount of levansucrose in a filtrate isolated from a glucan synthesis reaction.

The invention discloses a rapid pickle fermentation method based on gradient temperature control and flora directional fermentation, and relates to the field of biological fermentation. Comprising the following steps: S100, chopping vegetables, performing cleaning, performing deastringency treatment, and performing draining for standby application; s200, lactococcus lactis, leuconostoc mesenteroides and lactobacillus plantarum are prepared into a three-layer embedding bacterial agent, and the three-layer embedding bacterial agent and the vegetables are put into saline water together; s300, nitrogen is introduced into a system in the step S200 to enable the dissolved oxygen content to be smaller than or equal to 0.5 mg / L, and gradient temperature control fermentation is performed in three stages; and S400, after fermentation is completed, adding mother water and then refrigerating. The three-layer embedding microbial agent is accurately matched with three-stage gradient temperature control fermentation, so that the fermentation period is shortened to be less than 78 hours, the residual quantity of nitrite is less than or equal to 0.5%, and the content of flavor substances is effectively increased.

Leuconostoc lactis LEL168 is deposited at the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH under an accession number DSM 35134 in accordance with the Budapest Treaty. Provided are methods for increasing calcium absorption and inhibiting bone resorption using the Leuconostoc lactis LEL168, compositions for use in increasing calcium absorption and inhibiting bone resorption, uses of a composition in the manufacture of a medicament or a food product for increasing calcium absorption and inhibiting bone resorption in a subject, and pharmaceutical compositions for increasing calcium absorption and inhibiting bone resorption in a subject.

The invention relates to the technical field of microorganisms and particularly discloses compound bacteria for low-temperature silage as well as a preparation method and application of the compound bacteria. The invention discloses a compound bacterium for low-temperature ensiling. The compound bacterium is prepared by mixing animal lactic acid bacteria Lactobacillus plantarum strain ZS-1, plant lactic acid bacteria Lactobacillus plantarum strain RL-5 and Leuconostoc mesenteroides XS-1, and the compound bacterium is prepared by mixing the animal lactic acid bacteria Lactobacillus plantarum strain ZS-1, the plant lactic acid bacteria Lactobacillus plantarum strain RL-5, the Leuconostoc mesenteroides XS-1 and the Leuconostoc mesenteroides XS-1, the Leuconostoc mesenteroides XS-1 and the Leuconostoc mesenteroides XS-1 and the Leuconostoc mesenteroides XS-1. The compound bacteria provided by the invention are used for low-temperature silage of forage, and the prepared silage contains high-content organic acid, crude protein and nutritional ingredients; the number of lactic acid bacteria in the silage can be increased, and the number of molds and saccharomycetes can be reduced, so that the decay probability in the low-temperature silage and storage process can be effectively reduced, and the fermentation quality of the silage is improved.

Leuconostoc lactis LEL168 is deposited at the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH under an accession number DSM 35134 in accordance with the Budapest Treaty. Methods for increasing calcium absorption and inhibiting bone resorption using the Leuconostoc lactis LEL168, compositions for use in increasing calcium absorption and inhibiting bone resorption, uses of a composition in the manufacture of a medicament or a food product for increasing calcium absorption and inhibiting bone resorption in a subject, and pharmaceutical compositions for increasing calcium absorption and inhibiting bone resorption in a subject are also provided.

The present invention provides a strain ZW081, a composition, a preparation method and an application thereof, belonging to the field of microbial technology, specifically a strain of Heindrixella coagulans ( Heyndrickxia coagulans ) ZW081, deposited in the China Center for Type Culture Collection on October 21, 2024, with the deposit address being Wuhan University, Wuhan, China, with the deposit number being CCTCC NO: M 20242271; a microbial composition comprising strain ZW081, Lactobacillus kefir ZJ13, Acetobacter ZJ10, Lactobacillus paracasei ZJ22, Lactobacillus rhamnosus ZJ18, Leuconostoc mesenteroides ZJ09, Lactobacillus fermentans ZJ20 and Kluyveromyces ZJ19; a method for preparing the microbial composition; and use of the strain and composition in the preparation of lipid-lowering products, wherein the strain and composition have the function of lowering lipids and losing weight.