10 results about "Leuconostoc sp." patented technology

The invention discloses a method for synthesizing alpha-arbutin through biotransformation, and belongs to the technical field of biochemistry. A gene cloning technology is utilized, sucrose phosphorylase Spase in leuconostoc mesenteroides and sucrose phosphorylase gtfa in bifidobacterium adolescentis are screened and found through a Deep Molecules and DLKcat algorithm on a Uniprot database, novopro is utilized for codon optimization, then the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa are expressed on a plasmid vector PET-28a and are converted into an escherichia coli BL21 (DE3) strain, and the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa in the bifidobacterium adolescentis are obtained. An engineering strain for heterologous expression of the gene is constructed by taking the gene as a chassis cell. According to the process, sucrose and hydroquinone are taken as co-substrates, and bioconversion is carried out under the action of sucrose phosphorylase from different sources to obtain alpha-arbutin.

The present invention relates to a method for preparing a fermented guava leaf composition having antioxidant efficacy superior to that of conventional guava leaves by using lactic acid bacteria fermentation in a guava leaf extract. The present invention has an excellent effect of providing: the method for preparing a guava leaf composition fermented with lactic acid bacteria, in which lactic acid bacteria of Leuconostoc mesenteroides (MGE 3138), Lactobacillus plantarum (MGE 3143), and Leuconostoc citrium Cares-1 are inoculated into a guava leaf extract and fermented; and a food composition having improved antioxidant efficacy and palatability by using the composition.

The application discloses a Leuconostoc intestini and Lactococcus lactis composition and application, belongs to the field of microbial pharmacy, and relates to application of a Leuconostoc intestini and Lactococcus lactis composition to preparation of a drug for treating pulmonary fibrosis diseases; wherein the quantity of the Leuconostoc intestini and the Lactococcus lactis in the composition is equal in terms of colony forming units. Experimental results show that the Leuconostoc intestini and the Lactococcus lactis can effectively improve pulmonary fibrosis when being intervened alone or in combination. It is known to those skilled in the art that excessive deposition of collagen fibers is a core feature of pulmonary fibrosis diseases, and the Leuconostoc intestini and the Lactococcus lactis have a synergistic effect of inhibiting the content of lung tissue collagen in pulmonary fibrosis. Therefore, the Leuconostoc intestini and the Lactococcus lactis composition has a prospect of being developed into a drug for treating pulmonary fibrosis diseases (including idiopathic pulmonary fibrosis and secondary pulmonary fibrosis).

The present application relates to the technical field of microorganism, in particular to a leuconostoc lactis with good probiotic characteristics and application thereof in preparation of drugs for preventing and treating inflammatory bowel disease, and the leuconostoc lactis (Leuconostoc lactis) LL271 provided by the present application is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 25974.The strain has good acid tolerance, bile salt tolerance, self-aggregation ability, hydrophobicity and other probiotic characteristics such as the ability to inhibit common human pathogenic microorganisms; meanwhile, the strain can improve intestinal inflammation, has prevention and treatment effects on inflammatory bowel disease, has high biological safety, can be used as probiotic preparation for relieving inflammatory bowel disease, improving intestinal mucosal injury and colon inflammation, provides a new strategy for preventing and treating inflammatory bowel disease, and has a potential clinical application prospect.

The application discloses a kind of ginseng fermentation mixed culture ferment and application thereof.Belongs to leisure food preparation technical field.The mixed culture ferment includes lactobacillus plantarum LNJ-HQ-01, Leuconostoc mesenteroides LNJ-L-014 and lactobacillus fermentum BNCC194390.The application also discloses a kind of preparation method of hawthorn ginseng leisure food, as follows: hawthorn is washed, cored;after ginseng powder is mixed with water, the mixed culture ferment of the application is used to ferment in dark and sealed;after hawthorn is treated, ginseng is mixed and microwave heating, beating, sieving, and fruit paste is made;fruit paste is mixed with syrup;after being pressed into sheet, microwave intermittent low-speed pulsating airflow drying is used.The beneficial effects obtained by the application are as follows:the mixed culture ferment in the application can effectively avoid bacterial contamination, improve the nutritional value of ginseng, improve the bitter taste of ginseng, and the hawthorn ginseng leisure food prepared by the mixed culture ferment has good nutritional value and palatability.

The invention discloses a probiotic composition, a compound fermented feed and application, and belongs to the technical field of livestock and poultry breeding. Comprising leuconostoc mesenteroides and lactococcus, the leuconostoc mesenteroides is named as a leuconostoc mesenteroides LM01 strain and is preserved in China General Microbiological Culture Collection Center on November 21, 2025, the preservation number is CGMCC: 36737, and the lactococcus is named as a lactococcus LC01 strain and is preserved in China General Microbiological Culture Collection Center on November 21, 2025. The strain is preserved in the China General Microbiological Culture Collection Center on November 21, 2025, and the preservation number is CGMCC: 36736. The compound fermented feed disclosed by the invention is fermented by specific compound probiotics, so that the physicochemical properties and the nutritional value of the feed are effectively improved, and the compound fermented feed shows the effects of increasing the laying rate, the daily egg weight and the feed intake in laying hen breeding, and has extremely high popularization and application values.

A method for hydrolyzing an alpha-1,5 glucosyl-fructose bond in a levansucrose, e.g., a sucrose, is disclosed. The method comprises contacting the sucrose with an alpha-glucosidase, e.g., a transglucosidase or a glucoamylase, under suitable conditions, during which the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose bond of the sucrose. The method is used, e.g., to reduce the amount of levansucrose in a filtrate isolated from a glucan synthesis reaction.

Leuconostoc lactis LEL168 is deposited at the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH under an accession number DSM 35134 in accordance with the Budapest Treaty. Provided are methods for increasing calcium absorption and inhibiting bone resorption using the Leuconostoc lactis LEL168, compositions for use in increasing calcium absorption and inhibiting bone resorption, uses of a composition in the manufacture of a medicament or a food product for increasing calcium absorption and inhibiting bone resorption in a subject, and pharmaceutical compositions for increasing calcium absorption and inhibiting bone resorption in a subject.

The invention relates to the technical field of microorganisms and particularly discloses compound bacteria for low-temperature silage as well as a preparation method and application of the compound bacteria. The invention discloses a compound bacterium for low-temperature ensiling. The compound bacterium is prepared by mixing animal lactic acid bacteria Lactobacillus plantarum strain ZS-1, plant lactic acid bacteria Lactobacillus plantarum strain RL-5 and Leuconostoc mesenteroides XS-1, and the compound bacterium is prepared by mixing the animal lactic acid bacteria Lactobacillus plantarum strain ZS-1, the plant lactic acid bacteria Lactobacillus plantarum strain RL-5, the Leuconostoc mesenteroides XS-1 and the Leuconostoc mesenteroides XS-1, the Leuconostoc mesenteroides XS-1 and the Leuconostoc mesenteroides XS-1 and the Leuconostoc mesenteroides XS-1. The compound bacteria provided by the invention are used for low-temperature silage of forage, and the prepared silage contains high-content organic acid, crude protein and nutritional ingredients; the number of lactic acid bacteria in the silage can be increased, and the number of molds and saccharomycetes can be reduced, so that the decay probability in the low-temperature silage and storage process can be effectively reduced, and the fermentation quality of the silage is improved.

Leuconostoc lactis LEL168 is deposited at the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH under an accession number DSM 35134 in accordance with the Budapest Treaty. Methods for increasing calcium absorption and inhibiting bone resorption using the Leuconostoc lactis LEL168, compositions for use in increasing calcium absorption and inhibiting bone resorption, uses of a composition in the manufacture of a medicament or a food product for increasing calcium absorption and inhibiting bone resorption in a subject, and pharmaceutical compositions for increasing calcium absorption and inhibiting bone resorption in a subject are also provided.