35 results about "Leuconostoc sp." patented technology

Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and / or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.

The invention provides a segmented direct vat set chilli sauce composite leavening agent and a method for preparing chilli sauce by using the same, and belongs to the technical field of food fermentation. The composite leavening agent comprises a leavening agent 1 and a leavening agent 2, wherein the leavening agent 1 is prepared from freeze-dried powder of plant lactobacillus CICC 25125, freeze-dried powder of lactobacillus acidophilus CICC 6094 and freeze-dried powder of leuconostoc mesenteroides CICC 24385, and the leavening agent 2 is prepared from freeze-dried powder of saccharomyces cerevisiae, a fermentation accelerant and garlic essential oil. The composite leavening agent can effectively control the fermentation process, perform segmented intensified inoculation, increase the acid production rate, shorten the fermentation period and reduce the generation of nitrite in the fermentation process. The invention further provides a method for preparing the chilli sauce by using the composite leavening agent, and the prepared chilli sauce is low in nitrite content, presents soft and rich fruity flavor and is high in flavor.

The invention discloses a method for synthesizing alpha-arbutin through biotransformation, and belongs to the technical field of biochemistry. A gene cloning technology is utilized, sucrose phosphorylase Spase in leuconostoc mesenteroides and sucrose phosphorylase gtfa in bifidobacterium adolescentis are screened and found through a Deep Molecules and DLKcat algorithm on a Uniprot database, novopro is utilized for codon optimization, then the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa are expressed on a plasmid vector PET-28a and are converted into an escherichia coli BL21 (DE3) strain, and the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa in the bifidobacterium adolescentis are obtained. An engineering strain for heterologous expression of the gene is constructed by taking the gene as a chassis cell. According to the process, sucrose and hydroquinone are taken as co-substrates, and bioconversion is carried out under the action of sucrose phosphorylase from different sources to obtain alpha-arbutin.

The invention provides a new strain XP1 of Leuconostoc lactis, and the preservation number of the new strain XP1 of the Leuconostoc lactis is CGMCC (China General Microbiological Culture Collection Center) No.31789. The invention also provides a preparation method of the new strain XP1 of the Leuconostoc lactis. The novel leuconostoc lactis strain XP1 provided by the invention has relatively good pullulanase production activity and acid production characteristic; in a hemolysis experiment, no hemolysis phenomenon is generated, hemolysis is avoided, and safety and non-toxicity are achieved; the compound has drug resistance to rifampicin and has an inhibition effect on pathogenic bacteria such as staphylococcus aureus and escherichia coli; the strain XP1 has good oxidation resistance, the DPPH free radical scavenging rate is 85.20%, the superoxide anion free radical scavenging rate is 63.50%, the ABTS < + > free radical scavenging rate is 55.12%, and the strain XP1 has good application prospects in food development, health care product preparation and medicine preparation.

The invention relates to a preparation method of a leavening agent, in particular to a preparation method and application of a lactic acid bacteria leavening agent suitable for fermenting sweet potatoes. The preparation method of the lactic acid bacteria starter comprises the following steps: respectively and continuously activating lactobacillus plantarum, lactobacillus buchneri and leuconostoc mesenteroides for 2-3 times, compounding an activating solution according to the mass ratio of 1: 1: 1, and fermenting sweet potatoes at the most suitable fermentation temperature of 23.5 DEG C for 3 days with the inoculum size of the starter being 3.5%, thereby ensuring that the strains efficiently ferment the sweet potatoes in the most suitable environment. The preparation method of the lactic acid bacteria starter is simple, low in cost and capable of achieving industrial production, opens up a new way for sweet potato processing and utilization, adds a new variety for the sweet potato product market and promotes value increase and efficiency increase of the sweet potato industry.

Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and / or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.

The invention relates to a strain of leuconostoc lactis, which is named as leuconostoc lactis Gill 3, and is preserved in Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO.65904, the preservation address is building 5, building 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation date is February 18, 2025. The leuconostoc lactis can generate antibacterial substances with narrow-spectrum activity, the antibacterial range is more accurate, the leuconostoc lactis has a remarkable antibacterial effect on common gram-positive bacteria and partial negative pathogenic bacteria in various foods, but the leuconostoc lactis has weak resistance to other indicator bacteria or has no antibacterial activity; meanwhile, the antibacterial substance not only has an inhibition effect on bacterial planktonic cells, but also can resist the formation of bacterial biofilms. Therefore, the leuconostoc lactis disclosed by the invention can be used for prolonging the shelf life of food, and even can be developed into a probiotic.

The present invention relates to a method for preparing a fermented guava leaf composition having antioxidant efficacy superior to that of conventional guava leaves by using lactic acid bacteria fermentation in a guava leaf extract. The present invention has an excellent effect of providing: the method for preparing a guava leaf composition fermented with lactic acid bacteria, in which lactic acid bacteria of Leuconostoc mesenteroides (MGE 3138), Lactobacillus plantarum (MGE 3143), and Leuconostoc citrium Cares-1 are inoculated into a guava leaf extract and fermented; and a food composition having improved antioxidant efficacy and palatability by using the composition.

The invention discloses a bifidobacterium culture medium and a culture and preservation method based on a symbiotic nutrition method, and belongs to the technical field of probiotics. According to the bifidobacterium culture medium provided by the invention, by optimizing the raw materials and dosage ratio of the culture medium, not only is the cost of the culture medium reduced, but also the nutritional ingredients of the culture medium are more comprehensive and balanced, the adaptive capacity of bifidobacterium to environmental factors is enhanced, and the growth stability is ensured. According to the bifidobacterium culture method based on the symbiotic nutrition method, the growth and reproduction of the bifidobacterium can be promoted by utilizing the synergistic symbiosis of the bifidobacterium, the leuconostoc mesenteroides and the pediococcus acidilactici, and the adopted culture medium can meet different nutritional requirements of the three bacteria in the symbiotic culture process; the symbiotic cooperation among the bifidobacteria and the adaptive capacity to environmental factors are enhanced, the stability of symbiotic flora and the smooth proceeding of the culture process are ensured, the survival rate of the bifidobacteria in the preservation process is remarkably improved, and the storage life is prolonged.

To provide a novel trans epidermal water loss inhibitor and a moisturizing agent using the inhibitor.SOLUTION: The characteristics of the present invention in order to solve the problem are as follows. 1. A trans epidermal water loss inhibitor has a kudzu vine-derived lactic acid bacterium (Leuconostoc mesenteroides) as an active ingredient. 2. The trans epidermal water loss inhibitor described in 1 above in which the kudzu vine-derived lactic acid bacterium (Leuconostoc mesenteroides) is a lactic acid bacterium (Leuconostoc mesenteroides) (Patent Organisms Depositary accession number: NITE P-02751) strain and / or a variant thereof. 3. A skin roughness improver has the trans epidermal water loss inhibitor described in 1 or 2 above as an active ingredient. 4. A moisturizing agent has the trans epidermal water loss inhibitor described in 1 or 2 above as an active ingredient. 5. A horny layer moisturizing and barrier protein (involucrin, filaggrin, transglutaminase 1) expression promoter has a kudzu vine-derived lactic acid bacterium (Leuconostoc mesenteroides)(Patent Organisms Depositary accession number: NITE P-02751) strain and / or a variant thereof as an active ingredient.SELECTED DRAWING: None

The application discloses a Leuconostoc intestini and Lactococcus lactis composition and application, belongs to the field of microbial pharmacy, and relates to application of a Leuconostoc intestini and Lactococcus lactis composition to preparation of a drug for treating pulmonary fibrosis diseases; wherein the quantity of the Leuconostoc intestini and the Lactococcus lactis in the composition is equal in terms of colony forming units. Experimental results show that the Leuconostoc intestini and the Lactococcus lactis can effectively improve pulmonary fibrosis when being intervened alone or in combination. It is known to those skilled in the art that excessive deposition of collagen fibers is a core feature of pulmonary fibrosis diseases, and the Leuconostoc intestini and the Lactococcus lactis have a synergistic effect of inhibiting the content of lung tissue collagen in pulmonary fibrosis. Therefore, the Leuconostoc intestini and the Lactococcus lactis composition has a prospect of being developed into a drug for treating pulmonary fibrosis diseases (including idiopathic pulmonary fibrosis and secondary pulmonary fibrosis).

The present application relates to the technical field of microorganism, in particular to a leuconostoc lactis with good probiotic characteristics and application thereof in preparation of drugs for preventing and treating inflammatory bowel disease, and the leuconostoc lactis (Leuconostoc lactis) LL271 provided by the present application is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 25974.The strain has good acid tolerance, bile salt tolerance, self-aggregation ability, hydrophobicity and other probiotic characteristics such as the ability to inhibit common human pathogenic microorganisms; meanwhile, the strain can improve intestinal inflammation, has prevention and treatment effects on inflammatory bowel disease, has high biological safety, can be used as probiotic preparation for relieving inflammatory bowel disease, improving intestinal mucosal injury and colon inflammation, provides a new strategy for preventing and treating inflammatory bowel disease, and has a potential clinical application prospect.

The invention discloses leuconostoc mesenteroides capable of adjusting intestinal flora and application thereof, and belongs to the technical field of microorganisms. In order to research the influence of the leuconostoc mesenteroides on intestinal flora, a strain PM10 suspected of the leuconostoc mesenteroides is screened from bamboo shoots, and the strain is identified as the leuconostoc mesenteroides through morphological and molecular biological identification. According to the present invention, the acid resistance and bile salt resistance detection and the mouse intragastric administration experiment show that the leuconostoc mesenteroides PM10 has good acid resistance and bile salt resistance, and the relative abundance of the Bacteroides sp. Strain, the Lactobacillus sp. Strain and the Akkermansiasp. Strain in the mouse intestinal tract can be improved by using the viable bacteria and the dead bacteria of the leuconostoc mesenteroides PM10; as probiotics, the leuconostoc mesenteroides PM10 is expected to become a novel microbial preparation to be applied to production of functional foods, and has a huge application prospect.

The invention belongs to the field of micro-ecological feed additives, and discloses a leavening agent for whole corn silage. The leavening agent is composed of plant lactobacillus plantarum, lactobacillus brevis, leuconostoc mesenteroides and pediococcus acidilactici, strains of the plant lactobacillus plantarum, the lactobacillus brevis, the leuconostoc mesenteroides and the pediococcus acidilactici are fermented respectively, and then strain fermentation solutions obtained after fermentation are mixed according to the viable count ratio of (1-2): (1-2): (1-2): (1-2) to obtain the composite micro-ecological leavening agent. When the composite micro-ecological fermentation agent is used for whole-plant corn silage fermentation, the pH value can be rapidly reduced, the dry matter content can be increased, and the acid detergent fiber level can be reduced.

Disclosed herein is a method of making an antibiotic-resistant bacteria-free fermented food product to treat gut microbiota dysbiosis. Also disclosed is a fermented food product comprising a combination of unique starter strains of bacteria of Leuconostoc sp., Lactobacillus sp., Pediococcus sp., and / or other genera of lactic acid bacteria, such as Leuconostoc K6-Leu13 (WL-1) Leuconostoc mesenteroides Pediococcus K6-Pedio (WL-2), Pediococcus inopinatus, Lacticaseibacillus paracasei, Leuconostoc citreum, Lactobacillus SH16 (WL-3), Lactobacillus brevis, Pediococcus acidilactici, Latilactobacillus sakei, etc., for successful fermented products, with or without microbial succession.

The application discloses a kind of ginseng fermentation mixed culture ferment and application thereof.Belongs to leisure food preparation technical field.The mixed culture ferment includes lactobacillus plantarum LNJ-HQ-01, Leuconostoc mesenteroides LNJ-L-014 and lactobacillus fermentum BNCC194390.The application also discloses a kind of preparation method of hawthorn ginseng leisure food, as follows: hawthorn is washed, cored;after ginseng powder is mixed with water, the mixed culture ferment of the application is used to ferment in dark and sealed;after hawthorn is treated, ginseng is mixed and microwave heating, beating, sieving, and fruit paste is made;fruit paste is mixed with syrup;after being pressed into sheet, microwave intermittent low-speed pulsating airflow drying is used.The beneficial effects obtained by the application are as follows:the mixed culture ferment in the application can effectively avoid bacterial contamination, improve the nutritional value of ginseng, improve the bitter taste of ginseng, and the hawthorn ginseng leisure food prepared by the mixed culture ferment has good nutritional value and palatability.

The invention discloses a composite platycodon grandiflorum fermentation inoculant and a platycodon grandiflorum fermentation method thereof, and relates to a fermentation inoculant and a fermentation method thereof. The composite platycodon grandiflorum fermentation inoculant is formed by combining two or three of lactobacillus plantarum M3, lactobacillus brevis JW12 and leuconostoc mesenteroides ZS. The composite platycodon grandiflorum fermentation inoculant is used for platycodon grandiflorum fermentation, the content of platycodon grandiflorum total saponins and platycodon grandiflorum saponin D of the fermented platycodon grandiflorum decoction pieces is increased by more than one time, the mycoplasma pneumonia treatment effect of the platycodon grandiflorum decoction pieces is improved, and particularly, the composite platycodon grandiflorum fermentation inoculant has a remarkable callback effect on Treg / Th17 unbalance caused by mycoplasma pneumonia and promotes Treg / Th17 cell balance.

The invention relates to the technical field of land reclamation, and particularly discloses a land reclamation method for village style feature shaping. Comprising the following steps that 1, planting pits are positioned on the land and marked; 2, planting pits are dug according to the marks; 3) uniformly mixing the dug soil with a culture solution to obtain culture soil; step 4), standing and culturing the culture soil for 10-15 days to obtain planting soil; 5, water is injected into the planting pits, after no water is accumulated in the planting pits, part of the planting soil is backfilled, then loquat tree seedlings are planted, then the remaining planting soil is backfilled, watering maintenance is conducted for 30 days or longer, and land reclamation for village style feature shaping is completed. The culture solution is formed by compounding compound bacteria, water and nutrients; the compound bacteria are compounded from clostridium perfringens, lactobacillus acidophilus, kluyveromyces marxianus and leuconostoc mesenteroides. The method has the advantage that the survival rate of the loquat saplings on the derelict rural land is high.

The invention discloses a probiotic composition, a compound fermented feed and application, and belongs to the technical field of livestock and poultry breeding. Comprising leuconostoc mesenteroides and lactococcus, the leuconostoc mesenteroides is named as a leuconostoc mesenteroides LM01 strain and is preserved in China General Microbiological Culture Collection Center on November 21, 2025, the preservation number is CGMCC: 36737, and the lactococcus is named as a lactococcus LC01 strain and is preserved in China General Microbiological Culture Collection Center on November 21, 2025. The strain is preserved in the China General Microbiological Culture Collection Center on November 21, 2025, and the preservation number is CGMCC: 36736. The compound fermented feed disclosed by the invention is fermented by specific compound probiotics, so that the physicochemical properties and the nutritional value of the feed are effectively improved, and the compound fermented feed shows the effects of increasing the laying rate, the daily egg weight and the feed intake in laying hen breeding, and has extremely high popularization and application values.

According to the highland barley fermented beverage and the preparation method thereof, highland barley is used as a raw material, leuconostoc lactis XP1, bacillus velezensis and pichia pastoris are mixed for highland barley beverage fermentation, specific raw material ratio and enzymolysis reaction conditions are adopted, and strain inoculation and fermentation process conditions are optimized, so that the highland barley fermented beverage which is fine and smooth in mouth feel, rich in nutrition and rich in nutrition is obtained. The highland barley fermented beverage is moderate in sourness and sweetness and has grain fragrance. The obtained fermented beverage has good content indexes of total dietary fiber, resistant starch and protein, and no escherichia coli is detected. The technical scheme provided by the invention can provide theoretical basis and technical support for industrial production of the fermented highland barley beverage.

The invention discloses a biological complex microbial inoculant for growth of pitaya in the technical field of pitaya planting. The biological complex microbial inoculant comprises a marine bacterium complex liquid, the marine bacterium complex liquid comprises but is not limited to neoascomycetes, curvularia armbillata, sclerochaete hirsutum, mycoderma, Candicola incanus, streptomycete, streptomyces albidoflavus, leuconostoc mesenteroides, rhodococcus purpureus, lactococcus coriolis, solanum betulini, physotheca acuminata, Diapotheca subclavata, Diapotheca cf.nobilis, streptomycete hydride, streptomycete grassland, streptomycete and penicillium jerinckii, and the marine bacterium complex liquid is prepared from the marine bacterium complex liquid, the marine bacterium complex liquid, the marine bacterium complex liquid, the marine bacterium complex liquid, the marine bacterium complex liquid and the marine bacterium complex liquid. According to the scheme, the marine bacterial strain playing a role in promoting growth of pitaya plants is obtained through high-throughput sequencing screening, and the composite bacterial agent prepared by the scheme can promote growth of pitaya and increase the yield.

The invention discloses a composition of leuconostoc mesenteroides and lactococcus lactis and application thereof, belongs to the field of microbial pharmacy, and requests to protect application of the composition of leuconostoc mesenteroides and lactococcus lactis in preparation of drugs for treating pulmonary fibrosis diseases. Wherein in the composition, the number of the leuconostoc mesenteroides is equal to that of the lactococcus lactis in a colony forming unit. Experimental results show that pulmonary fibrosis can be effectively improved through independent or combined intervention of leuconostoc mesenteroides and lactococcus lactis. Technicians in the field know that excessive deposition of collagenous fibers is the core characteristic of pulmonary fibrosis lesion, and combined administration of leuconostoc mesenteroides and lactococcus lactis has the effect of synergistically inhibiting the collagen content of lung tissues in pulmonary fibrosis. Therefore, the composition of the leuconostoc mesenteroides and the lactococcus lactis has the prospect of being developed into medicines for treating pulmonary fibrosis diseases (including idiopathic pulmonary fibrosis and secondary pulmonary fibrosis).

The invention discloses a complex microbial inoculant containing bifidobacterium and application of the complex microbial inoculant. Comprising two or more of bifidobacterium lactis BL9, bifidobacterium longum subsp. Longum KS1, clostridium pratensis GZSY3214, Leuconostoc mesenteroides PM10, and Gluconacetobacter xylinus Q1, and further comprises a preparation method of the bifidobacterium lactis BL9, a preparation method of the bifidobacterium longum subsp. Longum KS1, a preparation method of the bifidobacterium longum subsp. Longum GZSY3214, a preparation method of the bifidobacterium longum, a preparation method of the bifidobacterium longum, a preparation method of the bifidobacterium longum and a preparation method of the bifidobacterium longum, and a preparation method of the bifidobacterium longum. The complex microbial inoculant can be used for preparing milk powder, weight-losing food and fruit and vegetable beverages, and is wide in application range.

A method for hydrolyzing an alpha-1,5 glucosyl-fructose bond in a levansucrose, e.g., a sucrose, is disclosed. The method comprises contacting the sucrose with an alpha-glucosidase, e.g., a transglucosidase or a glucoamylase, under suitable conditions, during which the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose bond of the sucrose. The method is used, e.g., to reduce the amount of levansucrose in a filtrate isolated from a glucan synthesis reaction.

The invention discloses a rapid pickle fermentation method based on gradient temperature control and flora directional fermentation, and relates to the field of biological fermentation. Comprising the following steps: S100, chopping vegetables, performing cleaning, performing deastringency treatment, and performing draining for standby application; s200, lactococcus lactis, leuconostoc mesenteroides and lactobacillus plantarum are prepared into a three-layer embedding bacterial agent, and the three-layer embedding bacterial agent and the vegetables are put into saline water together; s300, nitrogen is introduced into a system in the step S200 to enable the dissolved oxygen content to be smaller than or equal to 0.5 mg / L, and gradient temperature control fermentation is performed in three stages; and S400, after fermentation is completed, adding mother water and then refrigerating. The three-layer embedding microbial agent is accurately matched with three-stage gradient temperature control fermentation, so that the fermentation period is shortened to be less than 78 hours, the residual quantity of nitrite is less than or equal to 0.5%, and the content of flavor substances is effectively increased.

Leuconostoc lactis LEL168 is deposited at the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH under an accession number DSM 35134 in accordance with the Budapest Treaty. Provided are methods for increasing calcium absorption and inhibiting bone resorption using the Leuconostoc lactis LEL168, compositions for use in increasing calcium absorption and inhibiting bone resorption, uses of a composition in the manufacture of a medicament or a food product for increasing calcium absorption and inhibiting bone resorption in a subject, and pharmaceutical compositions for increasing calcium absorption and inhibiting bone resorption in a subject.

The invention relates to the technical field of microorganisms and particularly discloses compound bacteria for low-temperature silage as well as a preparation method and application of the compound bacteria. The invention discloses a compound bacterium for low-temperature ensiling. The compound bacterium is prepared by mixing animal lactic acid bacteria Lactobacillus plantarum strain ZS-1, plant lactic acid bacteria Lactobacillus plantarum strain RL-5 and Leuconostoc mesenteroides XS-1, and the compound bacterium is prepared by mixing the animal lactic acid bacteria Lactobacillus plantarum strain ZS-1, the plant lactic acid bacteria Lactobacillus plantarum strain RL-5, the Leuconostoc mesenteroides XS-1 and the Leuconostoc mesenteroides XS-1, the Leuconostoc mesenteroides XS-1 and the Leuconostoc mesenteroides XS-1 and the Leuconostoc mesenteroides XS-1. The compound bacteria provided by the invention are used for low-temperature silage of forage, and the prepared silage contains high-content organic acid, crude protein and nutritional ingredients; the number of lactic acid bacteria in the silage can be increased, and the number of molds and saccharomycetes can be reduced, so that the decay probability in the low-temperature silage and storage process can be effectively reduced, and the fermentation quality of the silage is improved.

Leuconostoc lactis LEL168 is deposited at the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH under an accession number DSM 35134 in accordance with the Budapest Treaty. Methods for increasing calcium absorption and inhibiting bone resorption using the Leuconostoc lactis LEL168, compositions for use in increasing calcium absorption and inhibiting bone resorption, uses of a composition in the manufacture of a medicament or a food product for increasing calcium absorption and inhibiting bone resorption in a subject, and pharmaceutical compositions for increasing calcium absorption and inhibiting bone resorption in a subject are also provided.

The invention discloses compound lactic acid bacteria for fermenting pickled potherb mustard as well as a preparation method and application of the compound lactic acid bacteria, and belongs to the technical field of food processing. In order to solve the problems of long fermentation time, limited temperature adaptability, large edible salt addition amount, high nitrite content, difficulty in anaerobic fermentation control and the like of the potherb mustard, the invention provides special efficient compound lactic acid bacteria for fermenting the potherb mustard, and the compound lactic acid bacteria comprise lactobacillus pentosus, leuconostoc mesenteroides and streptococcus thermophilus. The bacterial liquid of lactobacillus paracasei and bifidobacterium bifidum and the compound lactic acid bacteria are used for fermenting potherb mustard, so that the fermentation time can be shortened, the temperature adaptability can be improved, the dosage of edible salt can be reduced, the nitrite content of a finished product can be reduced, the fermentation flavor can be enriched, the fermentation stability can be improved, and the fermentation cost can be reduced.

The present invention belongs to the technical field of kimchi preparation, and specifically is a method for enhancing the aroma of industrial kimchi through multi-strain cooperative fermentation. The method comprises the following steps: (1) kimchi preparation; (2) lactic acid bacteria preparation, yeast preparation; (3) low-salt early fermentation; (4) high-salt late fermentation; (5) kimchi packaging. The present application adopts the cooperative regulation and control fermentation process technology of "inoculating lactic acid bacteria + yeast + environmental factors". The process adopts low salt and medium-low temperature in the early stage of fermentation, and the cooperative fermentation of enteric mesenteroides and Hansde Pasteurella candidiasis can quickly produce acid and enhance fragrance; in the middle and late stages of fermentation, high salt and medium temperature are adopted, and the cooperative fermentation of plant lactobacillus and oligosporic Kazakhstan yeast can quickly produce acid and enhance fragrance and simplify the flora, thereby stabilizing the quality. In the late stage of fermentation, the oligosporic Kazakhstan yeast consumes lactic acid to achieve acid control under high acidity, thereby achieving the purpose of preventing post-acidification. After the fermentation is completed, a rapid cooling process is adopted to inhibit microbial and enzyme activity and extend the shelf life.