Natural yeast leavening agent for bread
A technology of natural yeast and starter, applied in pre-baked dough treatment, microorganisms, fungi, etc., can solve the problems of obvious differences in flora between batches, unstable bread flavor, poor fermentation ability, etc., and achieve overall acceptability Improvement, significant nutritional value, and the effect of nutritional value improvement
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Embodiment 1
[0043] Embodiment 1: the preparation of natural yeast starter
[0044] (1) Preparation of lyophilized powder of Saccharomyces cerevisiae WJ-03, Leuconostoc lactis WJ-01, and Lactobacillus plantarum WJ-02
[0045] Saccharomyces cerevisiae WJ-03 was inoculated into 50mL YPD liquid medium with 1% inoculum amount, activated at 30°C and 220rpm for 14h, and then inoculated into YPD liquid medium with 5% inoculum amount, shaken at 30°C and 220rpm After 24 hours to the late logarithmic period, centrifuge at 8000 g for 10 minutes, wash the cells with an equal volume of sterile normal saline (0.85% Nacl) for 2 to 3 times, and concentrate 5 times in 10 mL of sterile normal saline.
[0046] Leuconostoc lactis WJ-01 was inoculated into 50 mL of GM17 liquid medium with 1% inoculum amount, cultured in a 30°C incubator for 12 hours, and then inoculated into 50mL GM17 liquid medium with 5% inoculum amount, and incubated at 30°C The incubator was cultured for 16 hours to the late logarithmic p...
Embodiment 2
[0081] Example 2: Preparation of Bread Using Natural Sourdough Starter
[0082] 16.65g of natural yeast starter (in which the number ratio of Saccharomyces cerevisiae: Leuconostoc lactis: Lactobacillus plantarum is 1:1.5:1.5), mix well with the appropriate amount of dry material in Table 1, add water and stir to form a ball, add starting Slowly stir the ghee at a speed of 140rpm for 1.5min, then quickly stir it at a speed of 280rpm for 2min until the gluten network structure is formed, and then cover and let stand for 10min at room temperature.
[0083] Divide the above dough into 90g each, and shape into a round shape. Proof for 2 hours in a proofing box (38°C, relative humidity 85%). Put the proofed dough into the oven (170°C on the upper heat, 210°C on the lower heat) and bake for 20 minutes.
[0084] Common yeast (ange yeast) bread was prepared in the same manner as above.
[0085] Sensory evaluation was carried out on the prepared bread, and the evaluation standard for...
Embodiment 3
[0099] Embodiment 3: Natural yeast starter shelf-life performance verification
[0100] The natural yeast starter was vacuum-packed and stored at 4°C. For water activity and survival rate during storage, see Figure 10 , it can be seen that the natural yeast starter can better maintain its water activity at a lower value (0.20-0.35). The survival rate of the natural yeast starter decreased slowly during storage (vacuum packaging, after 3 months of storage at 4°C, the survival rate was 81.78%±4.05%).
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