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Leuconostoc citreum strain and application thereof

A Leuconostoc lemon strain technology, applied in the field of microorganisms, can solve the problems of pathogenicity and less microorganisms, achieve significant technical advantages, improve preparation efficiency, and have good application prospects

Active Publication Date: 2021-09-24
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few microorganisms known to produce fructan sucrase, and a few microorganisms are pathogenic. In addition, almost all fructan sucrase producing bacteria can only synthesize fructan sucrose with one molecular weight Enzyme, therefore, looking for a bacterial strain of safe source, product (fructan sucrase) diversification satisfies the market and becomes an urgent problem to be solved in this area

Method used

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  • Leuconostoc citreum strain and application thereof
  • Leuconostoc citreum strain and application thereof
  • Leuconostoc citreum strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method of determining a poly sugar sucrose encoding gene, including the following:

[0032] Genome sequencing of lemonostoc citreum CGMCC No. 6431, found 2 continuously arranged fruit poly sucrose coded genes from all genome sequences, named 1291 and 1292, respectively (see figure 1 ), Its specific sequence is as follows.

[0033] 1291 gene sequence:

[0034] MNMKETTTRKKLYKSGKVWVAAATAFAVMGVSAVTTSISADTTASAVTAPTTADKAAPAVTAPTTADKAAPAVTAPTTADKAAPAVTAPTTADKAAPAVTAPTTADKATPAVAAPTTATSAQVTELTKNGLQLVSDAKIDNFDVNKLSSRQINSLNAAADKYYQNPNKTPNSNAVTYKNFDDLIKQLQEQPKNIAIPQFNSQNIKNLPAVTTESALTGKIEDLDIWDSWMVQDAQTQKVADVQGHQVMFALAGSTKEPADTHIYMLTTPYKATTINSWQMVGPVFGYNAVPWSQEWSGSATVNKDGSIQLFYTRVTWNDTIKQNMQRLSTINIVVRPTNSNGLAIENVNNDHIIFDGDGKYYQNIEQTVNSEGDNFTLRDPHVIEVDGQRYLSFETNTGTNNPEGYENVTDLSNYGGSLHYNVTKLLELVGNDAAFRTATLANGALGLLRLTQEQNNPTVTQIYDPLVTSNMVTDEIERANIVPLNGKYYLFTDTRLEKSSLGSNWNKSTASDIAMLGYVSDTLFGDYKPLNINGVVLVANKTSNDRTATYSYYAVPVDGYSDRLLITSYMSNRGMEAGNGLNATTAPSFIIQINADGTTAVEQTITTQNDWVDPYNAVDPSMY...

Embodiment 2

[0046] Example 2 Comparison of homology of cyclasia encoded gene

[0047] The software uses mega7 1291 and 1292 genes with other known similar gene (CAD48195.1, AAO14618.1, WP010237336, AAB97111.1, AC15886.1, BAA04475, AAC36458.1, CBJ48143.1, CCM43846.1, CPR14579. 1, EHD23269.1, AAT81165.1, AAL09386.1, all sequences from NCBI web site) homology comparison, as a result image 3 Shown, 1291 and 1292 genes with homology to known <30% of the same genes, suggesting that these two genes encoding levansucrase significant uniqueness.

Embodiment 3

[0048] Example 3 Detection of Fruit Sugar Characosase in Fermentation Liquid

[0049] 1, Materials and Methods

[0050] (A) Preparation of seed (Fermentation): A citreum (Leuconostoc citreum) CGMCCNO.6431 lyophilized powder was dissolved in sterile distilled water with a small amount, a ring with an inoculating loop take scribe containing 2% (w / v) sucrose on M17 agar (Merck Co., Germany), 28 deg.] C for 24h aerobic removed with an inoculating loop single colony was picked into 20 mL M17 broth containing 2% (w / v) sucrose (Merck Co. Germany), 28 ℃ 180rpm shaking for 24h removed, the culture was centrifuged at 9,000rpm 10 minutes, and the supernatant was discarded, cells were washed with sterile distilled water twice with sterile distilled water suspension of the original culture volume, to give seed fermentation, after testing, the seed liquid bacterial concentration of 1 × 10 9 CFU / ml.

[0051] (B) Preparation of tomato juice sucrose medium: ripe tomato washing, peeling, squ...

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Abstract

The invention discloses a Leuconostoc citreum strain and an application thereof. The classification name of the strain is Leuconostoc citreum, and the preservation number of the strain is CGMCC NO.6431. Two continuously arranged and normally expressed fructan sucrase coding genes are arranged on a genome of the strain. The fructan sucrase encoding gene encodes and expresses three fructan sucrases with different molecular weights. The molecular weights of the levan sucrase are 130 kDa, 90 kDa and 80 kDa correspondingly. Three kinds of natural fructan sucrase with different molecular weights can be obtained through single fermentation of the leuconostoc citreum, and the preparation efficiency of the fructan sucrase is greatly improved.

Description

Technical field [0001] The present invention relates to the field of microbiological technology, in particular a lemmon tartricilus strain and its application thereof. Background technique [0002] Levan is a kind of fructose molar polymer from plants or microorganisms, which typically contains a large amount of β- (2, 6) fructose bonds and a small amount containing a large amount of β- (2, 1) fructose bond. Composed of branches. Studies have shown that the microbial source Levan has anti-tumor, anti-diabetic, enhanced immunity, lowering blood fat, and other functions, and Levan can also be used in nanomaterials and pharmaceutical carriers. In order to improve the purity of the product Levan, the jugsucrase will typically extract from the microorganism, and then react with the substrate to obtain higher quality Levan. However, there is less microorganisms of the ygo glycose haclycose, and a few microorganisms have pathogenicity, in addition, almost all of the jugosachase enzyme p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/10C12R1/01
CPCC12N9/1055C12Y204/0101
Inventor 吴正钧刘振民韩瑨王晓花
Owner BRIGHT DAIRY & FOOD
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