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Water-insoluble exopolysaccharide of leuconostoc mesenteroides and preparation method thereof

A Leuconostoc enterococci, water-insoluble technology, applied in the biological field, can solve the problems of single type of glycosidic bond in the connection structure, cumbersome preparation methods, narrow sources of water-insoluble polysaccharides, etc., and achieve the effect of broad application prospects and high food safety

Active Publication Date: 2016-03-30
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is that the current source of water-insoluble polysaccharides is narrow, the connection structure of the branch chain and the main chain has a single type of glycosidic bond, the preparation method is cumbersome, and the strains used for the preparation are harmful bacteria. The present invention provides a Water-insoluble exopolysaccharide of leuconostoc enterococcus and preparation method thereof

Method used

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  • Water-insoluble exopolysaccharide of leuconostoc mesenteroides and preparation method thereof
  • Water-insoluble exopolysaccharide of leuconostoc mesenteroides and preparation method thereof
  • Water-insoluble exopolysaccharide of leuconostoc mesenteroides and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Example 1 Preparation of water-insoluble extracellular polysaccharide of Leuconostoc mesenteroides

[0033] 1. Materials and methods

[0034] Preparation of seeds (fermentation strains): dissolve the lyophilized powder of Leuconostocmesenteroides BD3749 with a small amount of sterile distilled water, and use an inoculation loop to take a circle and streak it on M17 solid medium containing 5% sucrose (purchase From OXOID, UK), take out aerobic culture at 30°C for 48h, pick a single colony with an inoculation loop and put it into 10mL M17 liquid medium containing 5% sucrose (purchased from OXOID, UK), and use a vortex shaker to evenly disperse the colonies In the liquid medium, 30℃, 180rpm shaking culture for 24h, take out, inoculate 2% (v / v) inoculum into 5% sucrose-containing M17 liquid medium (purchased from OXOID, UK), 30℃, 180rpm shaking culture After 24 hours, the culture was centrifuged at 15,000 rpm for 10 minutes, the supernatant was discarded, and the cells were was...

Embodiment 2

[0040] Example 2 Preparation of water-insoluble extracellular polysaccharide of Leuconostoc mesenteroides

[0041] 1. Materials and methods

[0042] Preparation of seeds (fermentation strains): the same as in Example 1.

[0043] Synthetic medium (peptone 1%, yeast extract 0.5%, K 2 HPO 4 0.5%, CaCl 2 0.034%, sucrose 30%, the percentages are mass percentages) Preparation: peptone 10g, yeast extract 5g, sucrose 300g, K 2 HPO 4 20g and CaCl 2 0.034g mixed with 1L distilled water, after fully dissolved, use NaHCO 3 Adjust the pH to 8 and sterilize to obtain a synthetic medium with the required sucrose concentration.

[0044] 2. Preparation of water-insoluble extracellular polysaccharide of Leuconostoc mesenteroides

[0045] The Leuconostoc mesenteroides seed liquid was inoculated into a synthetic medium with a sucrose mass percentage of 30% and a pH of 8 at an inoculum of 5% (v / v). The fermentation broth was obtained by shaking fermentation at 15°C and 300 rpm for 24 hours. After the ferme...

Embodiment 3

[0048] Example 3 Preparation of water-insoluble extracellular polysaccharide of Leuconostoc mesenteroides

[0049] 1. Materials and methods

[0050] Preparation of seeds (fermentation strains): the same as in Example 1.

[0051] Synthetic medium (peptone 1%, yeast extract 0.5%, K 2 HPO 4 0.5%, CaCl 2 0.034%, sucrose 5%, the percentages are mass percentages) Preparation: peptone 10g, yeast extract 5g, sucrose 50g, K 2 HPO 4 20g and CaCl 2 0.034g is mixed with 1L distilled water, after fully dissolving, adjust the pH to 5 with NaOH, and sterilize to obtain a synthetic medium with the required sucrose concentration.

[0052] 2. Preparation of water-insoluble extracellular polysaccharide of Leuconostoc mesenteroides

[0053] The Leuconostoc mesenteroides seed liquid was inoculated into a synthetic medium with a sucrose mass percentage of 5% and a pH of 5 at an inoculum amount of 1% (v / v). The fermentation broth was obtained by shaking fermentation at 37°C and 100 rpm for 96 hours. After th...

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Abstract

The invention discloses water-insoluble exopolysaccharide of leuconostoc mesenteroides and a preparation method thereof. The water-insoluble exopolysaccharide of leuconostoc mesenteroides is glucan of which the main chain is formed through connection of alpha-(1,6) glycosidic bonds and the branched chain is formed through connection of alpha-(1,3) and alpha-(1,4) glycosidic bonds, wherein the molar ratio of the glycosidic bonds alpha-(1,6), alpha-(1,3) and alpha-(1,4) is 1:0.2-0.3:0.3-0.4. Compared with other water-insoluble polysaccharide generated by bacterial strains coming from other sources, the water-insoluble exopolysaccharide possesses a special structure, and is a water-insoluble glucan simultaneously possessing alpha-(1,3) and alpha-(1,4) glycosidic bonds. The source of the water-insoluble exopolysaccharide is safe and reliable, the preparation method is relatively simple, is relatively suitable for industrialized production and application, and possesses extremely extensive application prospect.

Description

Technical field [0001] The invention belongs to the field of biological technology, and specifically relates to a water-insoluble extracellular polysaccharide of Leuconostoc mesenteroides and a preparation method thereof. Background technique [0002] Water-insoluble glucan (Insolubleglucan, IG) is a kind of carbohydrate polymer that is insoluble in aqueous solution and polymerized with glucose as a single component. This kind of carbohydrate with special physical and chemical properties is not only from a wide range of sources, but also in plants, The metabolites of fungi and bacteria have been reported, and there are many kinds. At present, there are three kinds of IGs that are widely reported: dextran, curdlan and cellulose. From the structural characteristics, the branches of these IGs that have been reported are only composed of α-(1,2), α-(1,3) or α-(1 , 4) One of the glycosidic bonds connects the branch to the main chain, and rarely involves the branch structure of two or...

Claims

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Application Information

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IPC IPC(8): C08B37/02C12P19/08C12R1/01
CPCC08B37/0009C12P19/08
Inventor 鄢明辉吴正均韩瑨徐晓芬冯华峰
Owner BRIGHT DAIRY & FOOD
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