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Liquid medium of aspergillus fumigatus

A technology of liquid culture medium and Aspergillus fumigatus, which is applied in the field of biomedicine, can solve the problems of Aspergillus fumigatus being easy to form balls, low reproducibility, and lack of nutrients in bacteria balls.

Active Publication Date: 2019-05-07
鲁南新时代生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the liquid fermentation of Aspergillus fumigates, Aspergillus fumigatus is very easy to form pellets and grow on the wall (Wen Yang et al., Effects of carboxymethylcel lulose and carboxypolymethylene on morphology of Aspergillus fumigates NRRL 2346 and Fumagillin Production, 2003, 24-27)
If the bacterial cells form small and uniform bacterial spheres, the viscosity can be reduced and the oxygen mass transfer can be improved. However, if the bacterial spheres are too large and non-uniform, it often leads to poor homogeneity and low reproducibility of the culture medium.
At the same time, excessively large bacterium balls will also cause a lack of nutrients inside the bacterium balls, thereby reducing metabolic yields

Method used

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  • Liquid medium of aspergillus fumigatus
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  • Liquid medium of aspergillus fumigatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Incline medium (g / L): glucose 20, yeast extract 20, tryptone 1, agar powder 20, pH 6.7.

[0033] Liquid medium: Glucose 30g / L, corn steep liquor 25g / L, prepared with purified water, pH adjusted to 6.5. Dispense into 500mL Erlenmeyer shaker flasks, the filling volume is 100mL. In the experimental group, 1.5g (15g / L) of different additives were added to the shake flasks.

[0034] Training method:

[0035] The spores were inoculated on slant medium and cultured at 30°C for 9 days. After the spores are mature, scrape the spores on the inclined surface and make 10 spores with sterile water. 6 -10 7 Each / mL spore suspension was inoculated in shake flask liquid culture medium according to 2.5% inoculum amount, the shake flask was placed on a shaker, and cultured at a temperature of 28° C. and a rotation speed of 240 rpm for 8 days, with three parallels in each group.

[0036] The experimental results are shown in Table 1. It can be seen from the results that the insolubl...

Embodiment 2

[0041] Slant medium (g / L): glycerol 7.5, sucrose 9.4, magnesium sulfate heptahydrate 0.05, dipotassium hydrogen phosphate 0.06, sodium chloride 4, tryptone 5, agar powder 20, pH 6.7.

[0042] Liquid medium (g / L): starch 45g / L, corn steep liquor 25g / L, prepared with purified water, pH adjusted to 6.5. Dispense into 500mL Erlenmeyer shaker flasks, the filling volume is 100mL. In the experimental group, different concentrations of calcium phosphate were added to the shake flasks.

[0043] Training method:

[0044] The spores were inoculated on slant medium and cultured at 30°C for 12 days. After the spores are mature, scrape the spores on the inclined surface and make 10 spores with sterile water. 6 -10 7 Each / mL spore suspension was inoculated in shake flask liquid culture medium according to 2.5% inoculum amount, the shake flask was placed on a shaker, and cultured at a temperature of 28° C. and a rotation speed of 240 rpm for 8 days, with three parallels in each group.

...

Embodiment 3

[0050] Slant medium (g / L): glycerol 7.5, sucrose 9.4, magnesium sulfate heptahydrate 0.05, dipotassium hydrogen phosphate 0.06, sodium chloride 4, tryptone 5, agar powder 20, pH 6.7.

[0051] Liquid medium: dextrin 45g / L, corn steep liquor 25g / L, prepared with purified water, adjusted to different pH values. Dispense into 500mL Erlenmeyer shaker flasks, the filling volume is 100mL. The experimental group added 20g / L of quartz sand or calcium phosphate.

[0052] Training method:

[0053]The spores were inoculated on slant medium and cultured at 30°C for 12 days. After the spores are mature, scrape the spores on the inclined surface and make 10 spores with sterile water. 6 -10 7 Each / mL spore suspension was inoculated in shake flask liquid culture medium according to 2.5% inoculum amount, the shake flask was placed on a shaker, and cultured at a temperature of 28° C. and a rotation speed of 240 rpm for 8 days, with three parallels in each group.

[0054] The experimental re...

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PUM

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Abstract

The invention belongs to the technical field of medicine, and provides a liquid medium of aspergillus fumigatus. Insoluble strong base weak acid salt powder is added to the liquid medium of aspergillus fumigatus NRRL2436, thereby reducing the formation of the bacterium balls during the liquid culture process of the aspergillus fumigatus. The bacteria are present in the form of fine bacterium ballsor dispersed hyphae, and the formation of bacteria of different sizes is reduced. Homogenization of the liquid culture of aspergillus fumigatus is achieved, and the fermentation titer of fumagillin is significantly improved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a liquid culture medium for Aspergillus fumigatus. Background technique [0002] Fumagillin is an antibiotic produced by Aspergillus fumigatus. It is polymerized from three isopentenes and belongs to secondary metabolites related to terpenes and steroids. Fumagillin has a special effect on microsporidiosis and amoebicosis. In addition, fumagillin has been shown to inhibit endothelial cell proliferation and angiogenesis, thereby possessing anticancer activity. Many derivative analogs of fumagillin can also inhibit angiogenesis and methionyl aminopeptidase activity, and some derivatives (XMT-1107, TNP-470, CKD-732, CKD-1061, PPI-2458) are effective in anti- Cancer and weight loss have entered clinical research. Therefore, the status of fumagillin as a pharmaceutical intermediate is becoming more and more important. [0003] Fumagillin can be obtained by liquid fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/68
Inventor 张贵民朱兵峰李阳
Owner 鲁南新时代生物技术有限公司
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