Preparation method for Sutong potato medium convenient for growth of bacillus calmette guerin floating membrane

A technology of potato medium and Sutong medium, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of difficult growth of BCG, increase of vertical distance, difficulty of subculture, etc., and achieve effective It is beneficial to growth and reproduction, reduces starch content, and is not easy to be soft and rotten.

Active Publication Date: 2019-05-07
成都可恩生物科技有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

The Sutong potato culture medium prepared according to this method causes potatoes to be easily paste after autoclaving, and the potatoes are soft and rotten, which makes it difficult to inoculate. The vertical distance increases, making it difficult for BCG to grow to the liquid level of the Sutong medium, resulting in difficulties in subculture

Method used

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  • Preparation method for Sutong potato medium convenient for growth of bacillus calmette guerin floating membrane
  • Preparation method for Sutong potato medium convenient for growth of bacillus calmette guerin floating membrane
  • Preparation method for Sutong potato medium convenient for growth of bacillus calmette guerin floating membrane

Examples

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Effect test

Embodiment 1

[0027] Take potatoes with starch content ≤ 13%, not easy to paste soup, cut into half cylinders with a diameter of 2.0cm and a length of 3.0cm with a mold. The cut-off point at 1 / 2 of the length of the half cylinder is cut into double slopes, with the upper slope forming an angle of 30° with the horizontal plane, and the lower slope forming an angle of 18° with the horizontal plane. Soak the cut potatoes in purified water for 10 minutes, then replace the purified water with boiling water for 15 minutes. Put the potato pieces into a test tube with a neck (2.5cm×20cm), add 10ml of Sutong medium into the test tube with a neck, plug in a breathable rubber stopper, and lean against the culture plate so that the upper slope of the potato pieces is horizontal, Su The medium is connected to the lower slope of the potato block, and the vertical distance below the upper slope of the potato block is 0.1 ~ 0.3 cm, and the following is obtained: figure 1 The Sutong potato culture medium s...

Embodiment 2

[0029] Take potatoes with starch content ≤ 13%, not easy to paste soup, cut into semi-cylindrical bodies with a diameter of 1.7cm and a length of 2.5cm with a mold. 2 / 5 of the length of the half cylinder is the dividing point, cut into double slopes, the upper slope and the horizontal plane present an angle of 28°, and the lower slope and the horizontal plane present an angle of 15°. Soak the cut potatoes in purified water for 8 minutes, then replace the purified water with boiling water for 10 minutes. Put the potato pieces into a test tube with a neck (2.5cm×20cm), add 8ml of Sutong medium into the test tube with a neck, plug in a breathable rubber stopper, and lean against the culture plate so that the upper slope of the potato pieces is horizontal, and the Sutong The medium is connected to the lower slope of the potato block, and the vertical distance below the upper slope of the potato block is 0.1 ~ 0.3 cm, and the following is obtained: figure 1 The Sutong potato cultu...

Embodiment 3

[0031] Take potatoes with starch content ≤ 13%, not easy to paste soup, cut into semi-cylindrical bodies with a diameter of 2.3 cm and a length of 3.5 cm with a mold. The 3 / 5 of the length of the half cylinder is the dividing point, cut into double slopes, the upper slope and the horizontal plane present an angle of 33°, and the lower slope and the horizontal plane present an angle of 20°. Soak the cut potatoes in purified water for 15 minutes, then replace the purified water with a boiling water bath for 12 minutes. Put the potato pieces into a test tube with a neck (2.5cm×20cm), add 12ml of Sutong medium into the test tube with a neck, plug in a breathable rubber stopper, and lean against the culture plate so that the upper slope of the potato pieces is horizontal, Su The medium is connected to the lower slope of the potato block, and the vertical distance below the upper slope of the potato block is 0.1 ~ 0.3 cm, and the following is obtained: figure 1 The Sutong potato cu...

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Abstract

The invention relates to a preparation method for a Sutong potato medium convenient for the growth of a bacillus calmette guerin floating membrane. The method provides novel preparation processes of aSutong potato medium which are required to be used in the subculture of bacillus calmette guerin; the method includes selecting potatoes which are not easy to burnt and with starch content being lessthan or equal to 13%, cutting the potatoes into semi-cylinders with diameters being 1.7-2.3 cm and lengths being 2.5-3.5 cm, and taking the length 2/5-3/5 of the semi-cylinders as demarcation pointsto cut into double inclined planes; performing soaking in purified water for 8-15 min, and then replacing the purified water so that effects of washing off starch to prevent the potatoes from enzymatic browning can be achieved; performing boiling water bath on the cut potatoes for 10-15 min to further prevent the potatoes from enzymatic browning; and stuffing the potatoes into a necked test tube (2.5 cm * 20 cm), adding 8-12 ml of a Sutong medium into the necked test tube, plugging a breathable rubber plug, performing placing under 116 DEG C, and performing autoclaving for 30 min. The floatingmembrane of bacillus calmette guerin cultured by the Sutong potato medium prepared by the method can be conveniently grown to a Sutong medium level, so that output can be enhanced; and the method issimple in preparation and convenient in inoculation and usage.

Description

technical field [0001] The invention belongs to the technical field of biological product production, and in particular relates to a preparation method of Sutong potato culture medium which is beneficial to the growth of BCG floating film. Background technique [0002] Bacillus Calmette-Guerin for treatment uses batches of BCG working seeds to collect bacteria after being cultured in Roche's egg medium, Sutong potato medium, Sutong medium, and Kaisu medium, and then add stabilizers and pack them into vials Freeze-dried immunotherapeutics. Studies have shown that BCG for treatment can enhance the activity of macrophages, enhance the ability of macrophages to kill tumor cells, activate T lymphocytes, and enhance the body's cellular immunity. It can be used for ①adjuvant treatment of tumors; ②prevention of tuberculosis; ③treatment of asthmatic bronchitis and prevention of colds in children. It is also used for the treatment of bladder carcinoma in situ and the prevention of r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12M1/24C12M1/00C12R1/32
Inventor 严秋萍任昭源马昭明陈山岭张雪莹吴盈盈李泳宏陈楠
Owner 成都可恩生物科技有限公司
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