Primer, probe and reagent kit for detecting mutation of 2312nd-2313rd sites of EGFR (epidermal growth factor receptor) gene

Abstract

The invention discloses a primer, probe and reagent kit for detecting mutation of 2312nd-2313rd sites of an EGFR (epidermal growth factor receptor) gene. The primer is as shown in SEQID No.1-SEQ ID No.2, and the probe is as shown in SEQID No.3. Real-time fluorescence PCR is performed through the reagent kit disclosed by the invention, so that the mutation of an ACCCCA basic group inserted in the 2312nd-2313rd sites of an EGFR gene can be detected.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer, a probe and a related kit for detecting EGFR gene mutation. Background technique [0002] The incidence of malignant tumors in China has been increasing year by year in recent years, with more than 1.7 million new patients each year. Lung cancer is one of the most common malignant tumors in the world, and 80%-85% of them are non-small cell lung cancer (NSCLC). In my country, lung cancer has become the first cause of death of malignant tumors, and the 5-year survival rate is about 15%. With the development of tumor molecular biology, targeted therapy has attracted much attention. Selecting appropriate patients for targeted therapy is the key to its success. Epidermal growth factor receptor (EGFR) is an important target of tumor therapy at present. point. [0003] The EGFR gene is located on the short arm of human chromosome 7 (7p12), which is about 118kb long and consists...

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Problems solved by technology

However, the low sensitivity of this method will lead to missed detection and false negative, and the detection time is long, which cannot meet the actual needs of clinical detection

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