Primer, probe and reagent kit for detecting mutation of 2312nd-2313rd sites of EGFR (epidermal growth factor receptor) gene
A kit and gene technology, used in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of false negatives, long detection time, low sensitivity, etc., and achieve strong specificity and detection. Fast and sensitive effects
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[0020] Below in conjunction with specific embodiment, the present invention is further elaborated. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. For the experimental methods without specific conditions indicated in the examples, the conventional conditions well known to those skilled in the art, or the conditions suggested by the manufacturer were followed.
[0021] 1. Principle
[0022] After the specific primers and probes of the present invention are combined with the DNA template, Taq DNA polymerase uses deoxynucleotide (dNTP) as a substrate to amplify the mutant gene of the internal reference gene (Internal Reference, IR) and the EGFR gene in vitro. increase. Fluorescent PCR technology is used for detection, and fluorescence is released through hydrolysis of specific probes to monitor the progress of PCR reaction and determine the mutation of EGFR gene.
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