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Domestication method of wild selenium-rich shitake strain

A technology for enriching selenium and shiitake mushrooms, which is applied in the fields of botanical equipment and methods, mushroom cultivation, horticulture, etc., can solve the problems of less domestication of wild selenium-enriched shiitake mushroom strains, low absorption and utilization rate of selenium fertilizer, and poor flavor and quality of shiitake mushrooms. problems, to achieve the effect of improving stress resistance, improving quality and flavor, and reducing the rate of degradation

Inactive Publication Date: 2019-05-10
绩溪县桐坑源家庭农场
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on selenium-enriched shiitake mushrooms mainly focuses on the cultivation of selenium-enriched shiitake mushrooms, and there are few studies on the domestication of wild selenium-enriched shiitake mushrooms.
At the same time, although the shiitake mushrooms studied and produced have a high selenium content, the flavor and quality of the shiitake mushrooms are poor, and the absorption and utilization rate of selenium fertilizers is low. There are also problems that the shiitake mushroom strains in production are easy to degrade, be polluted, and have poor stress resistance. The shortcomings, did not fundamentally solve the problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A domestication method of wild selenium-enriched shiitake mushroom strains, comprising the following steps:

[0027] S1. Mushroom selection: In the winter and spring season at 6-15°C, select wild shiitake mushrooms that are associated with trees in selenium-rich soil mountainous areas in Shitai County, Anhui Province, with strong growth, thick caps, no pests and diseases, and appropriate maturity. The body is a kind of mushroom, and put it into a sterile paper bag and bring it back.

[0028] S2. Mushroom pretreatment: remove the sundries on the surface of the selected mushrooms, and use cotton balls containing 75% alcohol to disinfect the surface of the canopy and stipe of the mushroom body, and then rinse them twice with sterile water. And dry the surface moisture of the mushroom body with sterilized absorbent cotton.

[0029] S3. Tissue separation: In the aseptic inoculation room, sterilize the scalpel with the flame of an alcohol lamp. After the scalpel cools down, ...

Embodiment 2

[0036] A domestication method of wild selenium-enriched shiitake mushroom strains, comprising the following steps:

[0037] S1. Mushroom selection: In the winter and spring season at 6-15°C, select wild shiitake mushrooms that are associated with trees in selenium-rich soil mountainous areas in Shitai County, Anhui Province, with strong growth, thick caps, no pests and diseases, and appropriate maturity. The body is a kind of mushroom, and put it into a sterile paper bag and bring it back.

[0038] S2. Mushroom pretreatment: remove the sundries on the surface of the selected mushrooms, and use a cotton ball containing 75% alcohol to disinfect the surface of the canopy and stipe of the mushroom body, and then rinse it with sterile water for 4 times. And dry the surface moisture of the mushroom body with sterilized absorbent cotton.

[0039] S3. Tissue separation: In the aseptic inoculation room, sterilize the scalpel with the flame of an alcohol lamp. After the scalpel cools d...

Embodiment 3

[0046] A domestication method of wild selenium-enriched shiitake mushroom strains, comprising the following steps:

[0047] S1. Mushroom selection: In the winter and spring season at 6-15°C, select wild shiitake mushrooms that are associated with trees in selenium-rich soil mountainous areas in Shitai County, Anhui Province, with strong growth, thick caps, no pests and diseases, and appropriate maturity. The body is a kind of mushroom, and put it into a sterile paper bag and bring it back.

[0048] S2. Mushroom pretreatment: Remove the sundries on the surface of the selected mushrooms, and use cotton balls containing 75% alcohol to disinfect the surface of the canopy and stipe of the mushrooms, and then rinse them with sterile water for 3 times. And dry the surface moisture of the mushroom body with sterilized absorbent cotton.

[0049] S3. Tissue separation: In the aseptic inoculation room, sterilize the scalpel with the flame of an alcohol lamp. After the scalpel cools down...

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Abstract

The invention relates to the technical field of the breeding of edible mushroom strains and particularly relates to a domestication method of a wild selenium-rich shitake strain. The domestication method comprises the following steps: (S2) seeding mushroom selection, (S2) seeding mushroom pretreatment, (S3) tissue separation, (S4) tissue culture, (S5) rotary tube culture and (S6) oblique surface repeated culture. According to the domestication method, a wild excellent shitake body concomitant with a tree in a selenium-rich mountainous area is taken as a seeding mushroom during the seed selection, tree leaf liquid and a selenium-rich nutrient solution are added into a culture medium in the domestication culture process, and the proportions of the tree leaf liquid and the selenium-rich nutrient solution are continuously adjusted, so that the adaptability of the wild selenium-rich shitake strain to the culture mediums, namely tree flour and the selenium-rich nutrient is improved, the absorption rate of the domesticated shitake strain to the selenium-rich nutrient is increased, the degeneration speed is decreased, the stress resistance is improved, the pollution possibility is reduced,and the pertinence is strong; and meanwhile, the quality and flavor of the shitake strain are improved, and the method is high in economic benefit and has practical guiding significances and popularization and application values.

Description

technical field [0001] The invention relates to the technical field of edible fungus strain breeding, in particular to a domestication method of wild selenium-enriched shiitake mushroom strains. Background technique [0002] The output of shiitake mushrooms ranks second in the world's artificially cultivated edible fungi. With the improvement of the quality of life, people began to gradually realize the nutritional value of shiitake mushrooms. Due to its unique flavor and the effect of enhancing human immune function, it has become one of people's favorite health foods. [0003] In recent years, due to the shortage of wild shiitake mushrooms in the market, shiitake mushrooms have gradually changed from natural growth in the wild to artificial domestication cultivation, so the output of shiitake mushrooms has increased significantly. In order to better meet the growing market demand, more and more Farmers use various chemical fertilizers and pesticides in order to increase p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20A01G18/40
Inventor 章荣春程叙海程扶旗章云时
Owner 绩溪县桐坑源家庭农场
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