Construction method and application of high-yield L-threonine gene engineering bacterium

A technology of genetically engineered bacteria and threonine, which is applied in the field of genetic engineering and microbial fermentation, can solve the problems of the theoretical conversion rate gap and other problems, and achieve the effects of saving fermentation costs, accelerating the growth of strains, and improving the conversion rate
CN109735478AActive Publication Date: 2019-05-10JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2019-05-10

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Abstract

The invention discloses a construction method and application of a high-yield L-threonine gene engineering bacterium and belongs to the technical field of gene engineering and microbial fermentation.By means of deletion of an arcA gene encoding a transcription regulatory factor, deletion of an iclR gene encoding transcription repression and deletion of a tdcC gene encoding a threonine transporter, the yield of L-threonine is greatly improved, the yield of L-threonine is increased to 20.1 g / L after 36 hours of fermentation, and the conversion rate reaches 0.67.
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Description

technical field

[0001] The invention relates to a construction method and application of a high-yielding L-threonine genetically engineered bacterium, belonging to the technical fields of genetic engineering and microbial fermentation. Background technique

[0002] L-threonine, formula C 4 h 9 NO 3 , relative molecular weight 119.12, white crystal or crystalline powder, is one of the essential amino acids for the human body. Threonine is a chiral compound, which has four isomers: L-threonine, D-threonine, L-allo-threonine and D-allo-threonine, which have physiological activity and natural Only L-threonine is present.

[0003] In the field of food, L-threonine is widely used as a food fortifier, which can improve the nutritional value of food, relieve fatigue, and promote growth and development. L-threonine can be used in combination with other amino acids to play an anti-oxidative effect, and it will produce a burnt aroma when heated together with glucose.

[0004] In ...

Claims

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