Fluorescent molecular marker of rice waxy gene Wx and application of fluorescent molecular marker

Abstract

The invention provides a fluorescent molecular marker of a rice waxy gene Wx and application of the fluorescent molecular marker. Primers of the fluorescent molecular marker comprise an RWx-FG primer,an RWx-FT primer, an RWx-R primer, a first universal primer and a second universal primer, wherein the sequence of the RWx-FG primer is shown in SEQ ID NO:1, the sequence of the RWx-FT primer is shown in SEQ ID NO:2, the sequence of the RWx-R primer is shown in SEQ ID NO:3, the sequence of the first universal primer is shown in SEQ ID NO:4, and the sequence of the second universal primer is shownin SEQ ID NO:5. According to the technical scheme, a test sample is subjected to PCR amplification only once, no electrophoresis detection is required, and amplification data is obtained by directlyusing a fluorescence scanner on an original plate; the operation is simple, convenient and rapid, the cost is low, the result is accurate, and the real closed-tube operation is achieved.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a fluorescent molecular marker of rice waxy gene Wx and an application thereof. Background technique [0002] Rice is an important food crop. More than 100 countries in the world grow rice, and two-thirds of the world's population uses rice as a staple food. With the development of social economy and the improvement of consumption level, people have higher and higher requirements for rice quality and safety. High-quality soft rice has the characteristics of good taste, high elasticity, and is not easy to harden after cold, which is loved by most consumers. An important factor affecting the cooking quality of rice is the quality of starch deposited in the endosperm, which is divided into amylose and amylopectin, and amylose is one of the main indicators for identifying rice quality. The amylose content in rice is high, the gelatinization temperature is often...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above technical problems, the present invention discloses a fluorescent molecular marker of the rice waxy gene Wx and its application. The detection sample is only amplified by PCR once without electrophoresis detection, and the amplification is directly obtained on the original plate with a fluorescent scanner. Data, Get Rice Wx a and Wx b genotype data; easy to operate, fast, low cost, accurate results, and solve the shortcomings of the currently used methods due to the time-consuming implementation process, cumbersome process, low efficiency and the use of toxic substances

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