Method for overcoming diploid potato self-incompatibility
A potato and diploid technology, applied in the field of biotechnology and genetic breeding
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[0073] Embodiment one: a kind of method for overcoming diploid potato self-incompatibility provided by the invention, comprises the steps:
[0074] (1) In potato self-incompatibility determining gene S-RNase gene S p3 and S p4 A target fragment is selected in the first exon region; in the step (1), the target fragment is located on the target gene S-RNase, and one chain of the target fragment has a nucleic acid sequence structure as shown in SEQ ID No.1. One strand of the target fragment has a nucleic acid sequence structure as shown in SEQ ID No.1. For example, wherein the target fragment is located on the target gene S-RNase, one strand of the target fragment has a 5'-(N) X -NGG-3' structure, (N) X Represents a base sequence {N with number X 1 ,N 2 ...N X}, N 1 ,N 2 ...N X Each in represents any one of the bases A, G, C, and T, and N in NGG is any one of the bases A, G, C, and T.
[0075] (2) According to the nucleic acid sequence of the target fragment obtained in...
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