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Cruciferae dominance apetalous gene and making method of dominance apetalous germplasm by gene

A cruciferous and petal technology, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve the problems of restricting non-petal materials, unable to prepare non-petal hybrid varieties, etc.

Active Publication Date: 2019-05-21
陕西省杂交油菜研究中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the petalless materials of cruciferous plants or crops such as rape and Arabidopsis are all from natural mutations. The petalless phenotype caused by the loss of AP3 gene function is a recessive inheritance, and it is impossible to prepare petalless hybrid varieties with this material. Thus, the application of this type of petalless material is limited

Method used

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  • Cruciferae dominance apetalous gene and making method of dominance apetalous germplasm by gene
  • Cruciferae dominance apetalous gene and making method of dominance apetalous germplasm by gene
  • Cruciferae dominance apetalous gene and making method of dominance apetalous germplasm by gene

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Embodiment 1

[0019] Example 1 Application of Rapeseed Dominant Petalless Gene in Innovation of Petalless Germplasm Resources

[0020] The inventor deleted the 6th exon (SEQ ID NO:5) of the wild-type rapeseed non-petal gene (i.e. BnAP3 (the nucleic acid sequence is SEQ ID NO:1)) of rapeseed, and obtained the mutant gene BnAP3-K (Nucleic acid sequence is SEQ ID NO: 2) Construct plant expression vector Cam-35S-BnAP3-K (Nucleic acid sequence is SEQ ID NO: 7) transform wild-type rape K407 (petal is normal) ( figure 1 B), while transforming the wild-type rape K407 with the expression vector Cam-35S-BnAP3 (the nucleic acid sequence is SEQ ID NO: 6) constructed by the wild-type rape without petal gene as a control ( figure 1 A). The results showed that the transgenic plants showed a typical petalless phenotype, and the stamens developed normally. This confirms that the cloned BnAP3-K gene is a dominant petalless gene, and can be used to create dominant petalless materials in rapeseed ( figure 1...

Embodiment 2

[0021] Example 2 Production of Arabidopsis thaliana dominant petalless gene and its application in the innovation of Arabidopsis petal variation germplasm resources

[0022] In order to further confirm that the gene obtained by deletion of the AP3 gene of petals or partial deletion of the sixth exon can be used to create dominant petalless materials of other cruciferous plants, the inventors deleted the AP3 gene of Arabidopsis thaliana through site mutation ( Namely the 6th exon (SEQ ID NO:5) of AtAP3), obtained the mutated AtAP3-K gene ( figure 2 ), and construct the plant expression vector Cam-35S-AtAP3-K (the nucleic acid sequence is SEQ ID NO: 9), the expression vector is transformed into the normal Arabidopsis Col-0 of petals, and the plant expression vector Cam is constructed with the normal AtAP3 gene simultaneously -35S-AtAP3 (the nucleic acid sequence is SEQ ID NO: 8) was transformed into wild-type Arabidopsis as a control ( image 3A). The results showed that: the...

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Abstract

The invention relates to the technical field of plant genetic engineering, in particular to the field of regulating genes of the plant petal phenotype and application of the genes. Specifically, the invention discloses a generation method of a cruciferae plant dominance apetalous mutant gene, a method for significantly reducing or losing of petals of cruciferous plants and application of the dominance apetalous gene of the cruciferous plants in generating a dominance petal-free phenotype germplasm material.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to the field of regulating genes of plant petal phenotype and its application. Specifically, the invention discloses a method for producing a dominant petalless gene and a dominant petalless mutant germplasm of a cruciferous plant, and its application in creating a dominant petalless germplasm material of a plant. Background technique [0002] The petals of cruciferous plants are an important medium for the transmission of sclerotinia. Therefore, the acquisition of petalless germplasm is of great significance for the cultivation of petalless varieties. [0003] For example, for rapeseed, existing studies have shown that the petal traits of rapeseed are controlled by 2-4 pairs of genes, including 2 pairs of major genes and 1-2 pairs of minor genes. Zhang Yanfeng et al. (2011) found that the phenotype of recessive petalless rapeseed is caused by the loss of function...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/20
Inventor 张彦锋穆建新朱彦涛王道杰董育红王晓峰陈娜乔幸安然
Owner 陕西省杂交油菜研究中心
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