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Polyoxin N component preparation method and detection method

A technology of polyoxin and detection method, which is applied in the field of detection of antibiotics and biopesticides, and can solve problems such as the deterioration of the ecological environment and the quality and safety of agricultural products

Inactive Publication Date: 2019-05-21
陕西麦可罗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the control of crop diseases, the long-term use of a large number of chemical pesticides will lead to the deterioration of the ecological environment and the occurrence of quality and safety problems of agricultural products. As a biological fungicide, polyoxin has the advantages of high efficiency, safety, low residue, and environmental friendliness. However, the use of bioassay methods by various enterprises in actual production affects the further promotion and use of polyoxin in the market

Method used

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  • Polyoxin N component preparation method and detection method
  • Polyoxin N component preparation method and detection method
  • Polyoxin N component preparation method and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Test equipment and conditions:

[0059] Waters 1525 high performance liquid chromatograph, Waters 2487 ultraviolet detector;

[0060] Chromatographic column: C18 embedded with polar groups (250×4.6mm, 5μm);

[0061] Mobile phase: trifluoroacetic acid: (V 水 +V 甲醇 ) = 3‰(v / v), where V 水 is 99.5%, V 甲醇 0.5%;

[0062] Flow rate: 1.0 mL / min;

[0063] Detection wavelength: 260nm;

[0064] Column temperature: 25°C;

[0065] Injection volume: 10 μL.

[0066] experiment procedure:

[0067] Weigh 0.02g of polyoxin N standard substance, dissolve in 50mL volumetric flask with pure water, dilute to volume, shake well, pass through

[0068] 0.45μm filter membrane, ready for the test standard solution.

[0069] Weigh a sample containing 0.02g of polyamycin N, dissolve it in a 50mL volumetric flask with pure water, constant volume, shake well, pass through a 0.45μm membrane filter, and prepare the test sample.

[0070] Get the above-mentioned test standard product and sample ...

Embodiment 2

[0072] Test equipment and conditions:

[0073] Waters 1525 high performance liquid chromatograph, Waters 2487 ultraviolet detector;

[0074] Chromatographic column: C18 embedded with polar groups (250×4.6mm, 5μm);

[0075] Mobile phase: trifluoroacetic acid: (V 水 +V 甲醇 ) = 3‰(v / v), where V 水 is 99.5%, V 甲醇 0.5%;

[0076] Flow rate: 1.0 mL / min;

[0077] Detection wavelength: 260nm;

[0078] Column temperature: 25°C;

[0079] Injection volume: 10 μL.

[0080] experiment procedure:

[0081] Weigh 0.02g of polyoxin N standard substance, dissolve in 50mL volumetric flask with pure water, dilute to volume, shake well, pass through

[0082] 0.45μm filter membrane, ready for the test standard solution.

[0083] Weigh a sample containing 0.02g of polyamycin N, dissolve it in a 50mL volumetric flask with pure water, constant volume, shake well, pass through a 0.45μm membrane filter, and prepare the sample for testing.

[0084] Mass Spectrometry Conditions

[0085] Instrumen...

Embodiment 3

[0093] Test equipment and conditions:

[0094] Waters 1525 high performance liquid chromatograph, Waters 2487 ultraviolet detector;

[0095] Chromatographic column: C18 embedded with polar groups (250×4.6mm, 5μm);

[0096] Mobile phase: 0.1% trifluoroacetic acid aqueous solution;

[0097] Flow rate: 1.0 mL / min;

[0098] Detection wavelength: 260nm;

[0099] Column temperature: 25°C;

[0100] Injection volume: 10 μL.

[0101] experiment procedure:

[0102] Weigh the standard product containing 0.02g polyoxin N, put it in a 50mL volumetric flask, dissolve it with pure water, dilute to volume, shake well, and pass through

[0103] 0.45μm filter membrane, ready for the test standard solution.

[0104] Weigh a sample containing 0.02g of polyamycin N, dissolve it in a 50mL volumetric flask with pure water, constant volume, shake well, pass through a 0.45μm membrane filter, and prepare the sample for testing.

[0105] Get the above-mentioned test standard product and sample s...

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Abstract

The invention relates to a polyoxin N component preparation method and a detection method. Through polyoxin N component preparation and efficiency detection, especially, through means such as detection condition optimization, the content of a component with specific efficacy is improved, and quality control on the polyoxin is more representative and operable.

Description

technical field [0001] The invention relates to the detection technology of antibiotic biological pesticides, in particular to a preparation method and detection method of polyoxin N component. Background technique [0002] Polyoxin, also known as polyoxin, is a variant of cocoa streptomycin aso in Japan ( S.cacaoi var.asoensis ) to isolate a nucleoside antibiotic. In my country, it is Streptomyces aureus ( S. aurea Chromogenes 4896) metabolites. [0003] Polyoxin is a broad-spectrum biological fungicide with good systemic conduction. Its mechanism of action is to interfere with the biosynthesis of chitin in the cell wall of the bacteria, so that the cell wall of the bacteria cannot be biosynthesized and lead to death, and it can also inhibit the sporulation of bacteria and the expansion of lesions. This ingredient is mainly used to control various plant diseases such as apple leaf spot defoliation, pear scab, grape gray mold, cucumber downy mildew, tomato late blig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 张楠潘忠成李昶志杨宏勃姬志勤李蒲民
Owner 陕西麦可罗生物科技有限公司