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Preparation method and a detection method for polyoxins C component

A technology of polyantimycin and a detection method, which is applied in the detection field of antibiotics and biological pesticides, can solve the problems of deterioration of the ecological environment, influence on the determination effect of polyantimycin, and quality and safety of agricultural products.

Inactive Publication Date: 2019-05-17
陕西麦可罗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Since the metabolites produced by Streptomyces aureus are multi-component, most companies currently use the double-disc bioassay method to identify the bactericidal effect of polyoxin on the indicator pathogenic bacteria. The test results are often affected by many factors such as different indicator bacteria for polyoxin potency bioassays by various companies, different components of polyoxin in different batches, and different bioassay errors in the same batch. Potency determination effect
[0007] On the other hand, in the prevention and control of diseases, the long-term use of a large number of chemical pesticides will lead to the deterioration of the ecological environment and the occurrence of quality and safety problems of agricultural products. As a biological fungicide, polyoxin has high efficiency, safety, low residue, and is environmentally friendly However, due to the fact that various enterprises adopt bioassay methods in actual production, the further promotion and use of polyoxin in the market will be affected.

Method used

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  • Preparation method and a detection method for polyoxins C component
  • Preparation method and a detection method for polyoxins C component
  • Preparation method and a detection method for polyoxins C component

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Test equipment and conditions:

[0060] Waters 1525 high performance liquid chromatograph, Waters 2487 UV detector;

[0061] Chromatographic column: C18 (250×4.6mm, 5μm) embedded with polar groups;

[0062] Mobile phase: trifluoroacetic acid: (V water +V Methanol )= 3‰(v / v), where V water Is 99.5%, V Methanol 0.5%;

[0063] Flow rate: 1.0 mL / min;

[0064] Detection wavelength: 260nm;

[0065] Column temperature: 25℃;

[0066] Injection volume: 10μL.

[0067] experiment procedure :

[0068] Weigh 0.02g polyoxin C standard product, dissolve it with pure water, make the volume constant, shake it in a 50mL volumetric flask, pass through a 0.45μm filter membrane, and prepare the test standard solution.

[0069] Weigh a sample containing 0.02g polyoxin C, dissolve it with pure water, make the volume constant, shake it in a 50mL volumetric flask, and pass it through a 0.45μm filter membrane to prepare a test sample.

[0070] Take the above test standards and sample solutions, inject the...

Embodiment 2

[0072] Test equipment and conditions:

[0073] Waters 1525 high performance liquid chromatograph, Waters 2487 UV detector;

[0074] Chromatographic column: C18 (250×4.6mm, 5μm) embedded with polar groups;

[0075] Mobile phase: trifluoroacetic acid: (V water +V Methanol )= 3‰(v / v), where V water Is 99.5%, V Methanol 0.5%;

[0076] Flow rate: 1.0 mL / min;

[0077] Detection wavelength: 260nm;

[0078] Column temperature: 25℃;

[0079] Injection volume: 10μL.

[0080] experiment procedure:

[0081] Weigh the standard substance containing 0.02g polyoxin C, dissolve it in a 50mL volumetric flask, dissolve it in pure water, dilute to volume, shake well, pass through a 0.45μm filter membrane, and prepare the test standard solution.

[0082] Weigh a sample containing 0.02g polyoxin C, dissolve it with pure water, make the volume constant, shake it in a 50mL volumetric flask, and pass it through a 0.45μm filter membrane to prepare the test sample.

[0083] Mass spectrometry conditions

[0084] In...

Embodiment 3

[0092] Test equipment and conditions:

[0093] Waters 1525 high performance liquid chromatograph, Waters 2487 UV detector;

[0094] Chromatographic column: C18 (250×4.6mm, 5μm) embedded with polar groups;

[0095] Mobile phase: trifluoroacetic acid: (V water +V Methanol )= 3‰(v / v), where V water Is 99.5%, V Methanol 0.5%;

[0096] Flow rate: 1.0 mL / min;

[0097] Detection wavelength: 260nm;

[0098] Column temperature: 25℃;

[0099] Injection volume: 10μL.

[0100] experiment procedure:

[0101] Weigh 0.02g polyoxin C standard product, dissolve it with pure water, make the volume constant, shake it in a 50mL volumetric flask, pass through a 0.45μm filter membrane, and prepare the test standard solution.

[0102] Weigh a sample containing 0.02g polyoxin C, dissolve it with pure water, dilute to volume, shake it in a 50mL volumetric flask, and pass it through a 0.45μm filter membrane to prepare a test sample.

[0103] Take the above-mentioned test standards and sample solutions, inject th...

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Abstract

The invention relates to a preparation method and a detection method for a polyoxins C component. Through component preparation and efficient detection of polyoxins C, especially optimization of detection conditions and other methods, the content of a specific efficacy component is further improved, and the quality control of polyoxins is more representative and operational.

Description

Technical field [0001] The invention relates to the detection technology of antibiotic biological pesticides, in particular to a preparation method and detection method of polyoxin C component. Background technique [0002] Polyoxin is also called polyoxin, which is derived from the cocoa streptomycin asol in Japan ( S.cacaoi var.asoensis A nucleoside antibiotic was isolated in ). In my country, it is Streptomyces achromogenes ( S.aurea chromogenes 4896) metabolites. [0003] Polyoxin is a broad-spectrum biological bactericide and has good systemic conduction. Its mechanism of action is to interfere with the biosynthesis of chitin in the cell wall of the bacterial cell, so that the cell wall of the bacterial cell cannot undergo biosynthesis and cause death, and it can also inhibit the spore production and the expansion of disease spots. This ingredient is mainly used to prevent and treat various plant diseases such as apple spot leaf fall disease, pear scab, grape gray mold, cu...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 潘忠成张楠田利明杨宏勃李蒲民
Owner 陕西麦可罗生物科技有限公司