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A kind of method for improving gibberellin ga4 and ga7 plate and frame yield

A gibberellin, plate and frame technology, applied in organic chemistry and other directions, can solve the problems of high environmental protection pressure, low solubility and high extraction cost, and achieve the effects of improving plate and frame yield, increasing filtration rate, and improving total yield.

Active Publication Date: 2022-08-05
江西新瑞丰生化股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method has the advantages of simple plate and frame operation and high clarity of the plate and frame filtrate after the protein structure is destroyed after the fermented mash is acidified, the protein structure is destroyed, and the filtrate of the plate and frame has the advantages of high clarity: one is that GA4 and GA7 are in acid The solubility is low under low conditions, and most of GA4 and GA7 still remain in the filter residue after acid adjustment, resulting in low plate and frame yield; the second is for the part of GA4 and GA7 remaining in the gibberellin mycelium in the fermentation mash, because Lack of enzymatic hydrolysis and wall-breaking process, so that all of it can not be released to the outside of the cell for extraction, and the yield of the plate and frame will also be low; the third is to use sulfuric acid to adjust the acid, and a large amount of sulfate ions remain in the waste liquid, which will put pressure on environmental protection. Large, if you use hydrochloric acid to adjust the pH, chloride ions will corrode the equipment, and the operation of hydrochloric acid is inconvenient, if you use other organic acids to adjust the pH, it will cause high extraction costs

Method used

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  • A kind of method for improving gibberellin ga4 and ga7 plate and frame yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) 60 cubic meters of gibberellin GA4+7 fermentation mash after fermentation is transferred to storage tank;

[0036] (2) in the storage tank, adjust the pH of the gibberellin GA4+7 fermentation mash to 7.0 with 20% sodium hydroxide solution;

[0037] (3) plate and frame filtration is carried out to the gibberellin GA4+7 fermentation mash after adjusting the pH to obtain a filtrate and a filter residue;

[0038] (4) the filter residue is washed, and when the titer of the washing solution is lower than 50 μg / ml, the washing is stopped to obtain a secondary filtrate;

[0039] (5) collect primary filtrate and secondary filtrate, detect filtrate titer, and the GA4+7 total 100 million collected in the filtrate is the total 100 million of the plate frame;

[0040] (6) Calculate the plate and frame yield.

[0041] Fermentation total 100 million (kg) The total number of plates and frames (kg) Filtrate transmittance Plate and Frame Yield 53.38 41.16 40％ ...

Embodiment 2

[0043] (1) 60 cubic meters of gibberellin GA4 fermentation broth after fermentation is transferred to storage tank;

[0044] (2) in the storage tank, adjust the pH of the gibberellin GA4 fermentation broth to 7.2 with 20% sodium hydroxide solution;

[0045] (3) Dilute the neutral protease with 300L of water and dissolve it before adding it to the storage tank. The enzyme activity of the neutral protease is 100,000 units / L, and the enzyme is hydrolyzed at 35°C for 1.5 hours;

[0046] (4) plate and frame filtration is carried out to the gibberellin GA4 fermentation broth after enzymolysis to obtain primary filtrate and filter residue;

[0047] (5) filter residue is washed to obtain secondary filtrate;

[0048] (6) collect primary filtrate and secondary filtrate, and the GA collected in the filtrate is the total 100 million of the plate and frame;

[0049] (7) Calculate the plate and frame yield.

[0050] Fermentation total 100 million (kg) The total number of plate...

Embodiment 3

[0052] (1) transfer the 63 cubic gibberellin GA4+7 fermentation broth after the fermentation to storage tank;

[0053] (2) in the storage tank, adjust the pH of the gibberellin GA4+7 fermentation broth to 7.2 with 20% sodium hydroxide solution;

[0054] (3) Dilute and dissolve the cellulase with 300L of water and then add it to the storage tank. The cellulase enzyme activity is 10,000 units / L, and the enzymatic hydrolysis is carried out at 35°C for 1.5 hours;

[0055] (4) plate and frame filtering the gibberellin GA4+7 fermentation broth after enzymolysis to obtain primary filtrate and filter residue;

[0056] (5) filter residue is washed to obtain secondary filtrate;

[0057] (6) collect primary filtrate and secondary filtrate, and the GA4+7 total 100 million collected in the filtrate is the total 100 million of the plate and frame;

[0058] (7) Calculate the plate and frame yield.

[0059] Fermentation total 100 million (kg) The total number of plates and frame...

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Abstract

The invention relates to the field of production and extraction methods for plant growth regulators, and specifically discloses a method for improving the yield of gibberellins GA4 and GA7 plates and frames. The method is suitable for gibberellin GA4 and GA4+7 fermentation systems, and the method comprises the following steps: adjusting pH: adjusting the pH of the gibberellin GA4 or GA4+7 fermentation mash with alkali to 6.5-7.5; enzymatic hydrolysis: adjusting the pH To the fermented mash after pH, a composite pretreatment enzyme composed of neutral protease, cellulase and lipase is added, and after mixing evenly, enzymatic hydrolysis is carried out for 0.5-1.5 h; flocculation: the fermented mash after enzymatic hydrolysis is added to 0.5‰-1.5 ‰ flocculant; Filtration: filter the flocculated fermented mash to obtain primary flocculation filtrate and flocculation residue; washing: wash the flocculation residue to obtain secondary flocculation filtrate; collect filtrate: combine primary flocculation filtrate and secondary flocculation filtrate The flocculation filtrate obtains the plate and frame filtrate. The application of the method of the invention can significantly improve the plate and frame yields of gibberellins GA4 and GA7 and the total extraction yield in the fermentation mash, and the process is simple to operate and has significant economic benefits.

Description

technical field [0001] The invention belongs to the field of production and extraction methods for plant growth regulators, in particular to a method for improving the yield of gibberellins GA4 and GA7 plates and frames, which is suitable for gibberellin GA4 and GA4+7 fermentation systems. Background technique [0002] Gibberellins (GAs) are a class of plant hormones belonging to diterpenoids, which are widely present in higher plants, fungi and bacteria. There are many kinds of gibberellins, and 136 kinds of gibberellins have been found so far, which are collectively referred to as gibberellins (GAs). Gibberellins have various regulatory functions as plant growth regulators. The gibberellins produced are different under different light, temperature conditions, different periods of plant growth and even different parts of the same plant. GA7 is mainly produced in shoot tips and immature seeds, while GA4 is found in roots, stems, leaves and seeds of plants. The activity of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/00
Inventor 聂志奎刘义雄宋志远周圣骄张金儿谷田聂波丰腾罗琴
Owner 江西新瑞丰生化股份有限公司